DETERMINANTS OF INTRACELLULAR pH IN THE ERYTHROCYTE

Warth, James A.; Desforges, Jane F.

doi:10.1111/j.1365-2141.1975.tb01833.x

Cited by 9 publications

(14 citation statements)

References 24 publications

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“…The intracellular hydrogen ion concentration of red blood cells is of paramount importance for it influences both glycolysis, necessary for erythrocyte function, and oxygen delivery, the main in uiuo function of the erythrocyte. Factors influencing intracellular pH (pH,) have recently been summarized (Warth & Desforges, 1975). The influence of pH, on sickle cell anaemia (SS) erythrocytes is particularly significant in view of its effect on the solubility of deoxygenated molecules of sickle haemoglobin (Hb S) (Magdoff-Fairchild et al, 1976), and the consequent sickling of SS erythrocytes (May & Huehns, 1976).…”

mentioning

confidence: 99%

Variability of Intracellular pH within Individual Populations of SS and AA Erythrocytes

1979

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Individual populations of AA and SS erythrocytes were fractionated according to cell density by centrifugation, and the fractions analysed for intracellular pH (PHi), the mole ratio of 2,3-diphosphoglycerate to haemoglobin (DPG:Hb), and cell concentration of haemoglobin (MCHC). The pHi of SS erythrocytes was consistently lower than that of AA erythrocytes throughout the density range, and the lowest pHi of both cell types (AA and SS) was found in cells with the highest density. As the highest density AA and SS erythrocytes are characterized by the lowest DPG:Hb values, their relatively low pHi cannot be ascribed to intracellular organic phosphate. Instead we propose that a redistribution of hydrogen ions across the membrane of both AA and SS erythrocytes is the ultimate result of progressive alterations in these membranes in vivo.

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confidence: 99%

Variability of Intracellular pH within Individual Populations of SS and AA Erythrocytes

1979

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“…Cardiovascular risk assessment has great importance for exercise prescription with the goal of improving clinical outcomes [43]. Table 1 outlines the primary indications for considering EST as an evaluative tool [43,44].…”

Section: Indication Of Exercise Stress Test For Diagnosis or Prognosismentioning

confidence: 99%

“…Absolute indications are clear, where relative indications may be superseded by the supervisor of EST. Table 3 outlines the indications of terminating EST [44,50].…”

Section: Termination Of Exercise Stress Testmentioning

confidence: 99%

The diagnostic value of exercise stress testing for cardiovascular disease is more than just st segment changes: A review

Sharif¹,

Alway²

2016

J Integr Cardiol

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Purpose: The objectives of this review were to: (1) identify the acute cardiovascular responses to aerobic exercise used in an exercise stress test (EST), (2) identify when and how to best conduct an EST including the stopping point, (3) identify conditions that would preclude an EST, (4) define the limiting signs and symptoms associated with an EST (5), discuss what data are needed as part of an EST and how to interpret EST data. Methods:We identify the cardiovascular responses to an EST and when an EST should or should not be used. Finally we analyze the components of an EST and potential sensitivities, significances and predictability of the changes in the outcome data that will provide diagnosis overt cardiovascular disease, and for purposes of exercise prescription and evaluation of rehabilitation effects in cardiac patients.Results: Although most of the focus for performing an EST traditionally has been on the diagnostic value to identify ST-segment abnormalities, an EST provides other valuable diagnostic and prognostic data including maximal oxygen consumption (predicted or measured), exercise capacity and fatigability, blood pressure and heart rate responses to exercise and recovery. Conclusions:This review identifies the most recent standards, and guidelines of EST, which include the acute physiological response of the cardiovascular system to aerobic exercise, the use of EST in evaluation of coronary artery disease, an updated diagnostic and prognostic variables of EST, and reporting of EST.

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“…In this regard, several reports have shown that CSCs contain several classical mechanisms to escape the cell death from cytotoxic insults [98,99]. Elevated apoptosis resistance, drug-efflux pumps, enhanced efficiency of DNA repair, detoxification enzyme expression and relative dormancy/slow cell cycle kinetics, all include mechanisms known to be used by the CSCs in GI-cancers [98–101]. For example, CSCs demonstrate higher apoptotic threshold and elevated numbers of the ATP-binding cassette (ABC) transporter family proteins, also known as drug resistance pump [101].…”

Section: Why Chemo And/or Radiation-therapies Fail To Kill Cancer Stementioning

confidence: 99%

“…Both, acute and chronic hypoxia increase radio-resistance among GI-cancer cells by evading cell cycle arrest [103]. In accordance, a key outcome of the CSCs resistance to radiation and chemotherapy is selection of more resistant CSCs clonal subpopulation within a heterogeneous CSCs population [98,104,105]. In this regard, CD133 + CSCs were preferably enriched in chemotherapy-resistant liver, pancreatic, colorectal and gastric cancers [106–108].…”

Section: Why Chemo And/or Radiation-therapies Fail To Kill Cancer Stementioning

confidence: 99%

Cancer Stem Cell and Gastrointestinal Cancer: Current Status, Targeted Therapy and Future Implications

Ahmad¹,

Dhawan²

2015

Biochem Pharmacol (Los Angel)

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The cancer stem cells (CSCs) are biologically distinct subset of rare cancer cells with inherent ability of self-renewal, de-differentiation, and capacity to initiate and maintain malignant tumor growth. Studies have further reported that CSCs prime cancer recurrence and therapy resistance. Therefore, targeting CSCs to inhibit cancer progression has become an attractive anti-cancer therapeutical strategy. Recent technical advances have provided a greater appreciation of the multistep nature of the oncogenesis and also clarified that CSC concept is not universally applicable. Irrespective, the role of the CSCs in gastrointestinal (GI) cancers, responsible for the most cancer-associated death, has been widely accepted and appreciated. However, despite the tremendous progress made in the last decade in developing markers to identify CSCs, and assays to assess tumorigenic function of CSCs, it remains an area of active investigation. In current article, we review findings related to the role and identification of CSCs in GI-cancers and discuss the crucial pathways involved in regulating CSCs populations’ development and drug resistance, and use of the tumoroid culture to test novel CSCs-targeted cancer therapies.

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DETERMINANTS OF INTRACELLULAR pH IN THE ERYTHROCYTE

Cited by 9 publications

References 24 publications

Variability of Intracellular pH within Individual Populations of SS and AA Erythrocytes

Variability of Intracellular pH within Individual Populations of SS and AA Erythrocytes

The diagnostic value of exercise stress testing for cardiovascular disease is more than just st segment changes: A review

Cancer Stem Cell and Gastrointestinal Cancer: Current Status, Targeted Therapy and Future Implications

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