Determinants of immunity to murine salmonellosis: studies involving immunization with lipopolysaccharide-lipid A-associated protein complexes in C3H/HeJ mice

Killion, James W.; Morrison, David C.

doi:10.1111/j.1574-6968.1988.tb02489.x

Cited by 9 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunization of mice with LPS was carried out twice by subcutaneous injection of 1.0–2.5 μg emulsified in Freund’s incomplete adjuvant (Difco, Franklin Lakes, NJ, USA) [ 25 , 26 ]. The immune serum and control serum were used 4 weeks after immunization of mice with LPS, or with adjuvant only.…”

Section: Methodsmentioning

confidence: 99%

Specific Monoclonal Antibody Overcomes the Salmonella enterica Serovar Typhimurium’s Adaptive Mechanisms of Intramacrophage Survival and Replication

et al. 2016

View full text Add to dashboard Cite

Salmonella-specific antibodies play an important role in host immunity; however, the mechanisms of Salmonella clearance by pathogen-specific antibodies remain to be completely elucidated since previous studies on antibody-mediated protection have yielded inconsistent results. These inconsistencies are at least partially attributable to the use of polyclonal antibodies against Salmonella antigens. Here, we developed a new monoclonal antibody (mAb)-449 and identified its related immunogen that protected BALB/c mice from infection with Salmonella enterica serovar Typhimurium. In addition, these data indicate that the mAb-449 immunogen is likely a major protective antigen. Using in vitro infection studies, we also analyzed the mechanism by which mAb-449 conferred host protection. Notably, macrophages infected with mAb-449-treated S. Typhimurium showed enhanced pathogen uptake compared to counterparts infected with control IgG-treated bacteria. Moreover, these macrophages produced elevated levels of pro-inflammatory cytokine TNFα and nitric oxide, indicating that mAb-449 enhanced macrophage activation. Finally, the number of intracellular bacteria in mAb-449-activated macrophages decreased considerably, while the opposite was found in IgG-treated controls. Based on these findings, we suggest that, although S. Typhimurium has the potential to survive and replicate within macrophages, host production of a specific antibody can effectively mediate macrophage activation for clearance of intracellular bacteria.

show abstract

Section: Methodsmentioning

confidence: 99%

Specific Monoclonal Antibody Overcomes the Salmonella enterica Serovar Typhimurium’s Adaptive Mechanisms of Intramacrophage Survival and Replication

et al. 2016

View full text Add to dashboard Cite

show abstract

“…For example, it is well established that isolation of LPS from gramnegative bacteria is often associated with coisolation of microbial outer membrane proteins, usually tightly bound to the LPS molecular complex (21). Further, the demonstrated biological activity of such proteins in stimulating cells of the immuneinflammatory system is well documented (12,18,22). Evidence from animal models of infection with gram-negative bacteria indicates that antibiotics which release greater quantities of LPS may result in higher mortality (5).…”

Section: Discussionmentioning

confidence: 99%

Comparable Endotoxic Properties of Lipopolysaccharides Are Manifest in Diverse Clinical Isolates of Gram-Negative Bacteria

Luchi

Morrison²

2000

Infect Immun

Self Cite

View full text Add to dashboard Cite

In general there is a poor correlation between serum lipopolysaccharide (LPS; the biologically active constituent of endotoxin) levels and mortality in septic patients. The objective of this study was to determine if chemical, structural, or biological differences among LPS from different clinical isolates of gram-negative bacteria might explain this discrepancy. LPS preparations were made using the hot phenol-water extraction method from eight clinical isolates of gram-negative bacteria. As a percentage of the total weight of the LPS, the phosphate content ranged from 3.0 to 13.8% (average, 6.7 ؎ 3.6%), and the 2-keto-3-deoxyoctonate content ranged from 1.9 to 27.4% (average, 8.9 ؎ 8.5%). These values were not dissimilar to those obtained for a reference endotoxin. In a standard measure of LPS activity, the Limulus amoebocyte lysate assay, there was approximately a twofold difference between the least and most active preparations. The two preparations with the greatest difference in their ability to elicit the secretion of tumor necrosis factor alpha from a mouse peritoneal macrophage cell line were similar in lethality when administered to mice sensitized to the effects of LPS by D(؉)-galactosamine. These relatively minor differences in LPS activity seem unlikely to explain the generally observed discrepancy between serum endotoxin levels and mortality in patients with gram-negative sepsis.

show abstract

“…Intensive CMI is induced by live avirulent Salmonella strains (25), whereas humoral immunity is elicited by killed virulent cells or lipopolysaccharides (1,10,21). With respect to CMI, a little understood aspect is the mechanism by which live bacteria induce stronger CMI than do killed bacteria.…”

mentioning

confidence: 99%

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity

et al. 1992

View full text Add to dashboard Cite

The ability of the H202-induced catalase of Salmonella typhimurium to induce cell-mediated immunity against S. typhimurium infection in mice was examined. When exponentially growing cells of S. typhimurium were treated with 20 ,uM H202, the cells resisted killing by 1 mM H202 and showed the induction of a new species of catalase in addition to the constitutively produced one. Two molecules of catalases in S. typhimurium were isolated from mutant strains: H202-induced catalase (catalase II, 320 kDa), from a regulatory gene-deficient oxyRI mutant, and constitutive catalase (catalase I, 350 kDa), from a katG gene-deleted mutant. When mice were inoculated with a sublethal dose of live cells, an intensive protective immunity (100%b survival at 3 weeks) after challenge with a virulent strain associated with the delayed-type footpad hypersensitivity (DTH) reactions to both catalase I and catalase II was induced. Conversely, mice immunized with formalin-killed virulent S. typhimurium did not elicit protective immunity or DTH to either catalase. When mice were immunized with catalase I or catalase II, an enhanced protection (to a certain extent: 50% survival at 3 weeks) was induced in mice immunized with catalase II associated with DTH which did not cross-react with catalase I but not in those given catalase I. These results suggest that H202-induced stress proteins, including catalase II, are the dominant antigens for cell-mediated immunity in live cells of S. typhimurium and that a burst of such stress proteins in live salmonellae in phagocytes is responsible for the induction of cell-mediated immunity that is largely involved in the protection of susceptible mice against Salmonella infection.

show abstract

Determinants of immunity to murine salmonellosis: studies involving immunization with lipopolysaccharide-lipid A-associated protein complexes in C3H/HeJ mice

Cited by 9 publications

References 33 publications

Specific Monoclonal Antibody Overcomes the Salmonella enterica Serovar Typhimurium’s Adaptive Mechanisms of Intramacrophage Survival and Replication

Specific Monoclonal Antibody Overcomes the Salmonella enterica Serovar Typhimurium’s Adaptive Mechanisms of Intramacrophage Survival and Replication

Comparable Endotoxic Properties of Lipopolysaccharides Are Manifest in Diverse Clinical Isolates of Gram-Negative Bacteria

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity

Contact Info

Product

Resources

About