Determinants of groundnut rosette virus disease occurrence in Uganda

Mugisa, Immaculate; Karungi, J.; Akello, B.; Ochwo-Ssemakula, Mildred; Biruma, M.; Okello, David Kalule; Otim, Godfrey A.

doi:10.1016/j.cropro.2015.10.019

Cited by 9 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be attributed to the high vector pressure during the short rains as compared to the long rains season when the aphid pressure is low as a result of heavy rains that wash the insects away. A study by [12], found that periods of long rains negatively affected GRD progression as aphid vector pressure was low. [19], reported a positive correlation between potato disease incidence and aphid numbers.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Incidence of Groundnut Rosette Disease (GRD) and Genetic Diversity of Groundnut Rosette Assistor Virus (GRAV) in Western Kenya

Mukoye¹,

Ndong’a²,

Wéré³

2019

IJGG

View full text Add to dashboard Cite

This study determined the incidence of groundnut rosette disease (GRD) and genetic diversity of groundnut rosette assistor virus (GRAV, genus Luteovirus) in western Kenya. The diseases is a major constraint of groundnuts in Sub-Saharan Africa (SSA) causing up to 100% yield losses in severe cases. Among the GRD associated viruses, GRAV plays a crucial role in vector transmission of the other viruses. Therefore understanding the genetics of GRAV across SSA could enhance development of resistance to the disease. In Kenya, groundnuts are mainly grown in western region, however, the yields are poor mainly due to GRD. Information on occurrence and distribution of GRD in western Kenya was not documented and little was known about the characteristics of associated viruses. Two diagnostic surveys were conducted in six counties; Bungoma, Busia, Homabay, Kakamega, Siaya and Vihiga. Symptomatic and asymptomatic groundnut were collected in RNAlater® solution for laboratory analysis. Total RNA was extracted from the leaf samples using RNeasy Mini Kit (Qiagen) according to the manufacturers' protocol and used for double stranded cDNA synthesis using the SuperScript II kit. The cDNA was column-purified with the DNA Clean & ConcentratorTM-5-DNA kit. The samples were then processed with the transposon-based chemistry library preparation kit (Nextera XT, Illumina) following manufacturer's instructions. The fragment sizes structure of the DNA libraries was assessed using the Agilent 2100 Bioanalyzer. The indexed denatured DNA libraries were sequenced (200-bp paired-end sequencing) on the Illumina MiSeq platform (Illumina). Reads quality check was done using FastQC. Trimmed reads were used for de novo assembly and contigs aligned to the viral genomes database using CLC Genomics Workbench 10.1.2. The assembled contigs were subjected to a BLASTn search against the GenBank database. Phylogenetic analyses and comparisons were performed using the MEGA X. Average incidence was 53% and 41% in the short and long rain seasons, respectively. Chlorotic rosette was the dominant symptom followed by Green rosette and Mosaic. The GRAV coat protein (GRAV-CP) gene sequences revealed 97-100% identity with GeneBank isolates showing very slight variations across SSA. The study concludes that GRD incidence is high in western Kenya and that GRAV is highly conserved across SSA. The study recommends an urgent need to curb GRD, possibly through the exploitation of pathogen derived resistance (PDR) with GRAV as the suitable candidate.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Both chlorotic and green rosette symptoms occur throughout the SSA, and sometimes occur in the same field [12]. A less common third symptom variant, called mosaic rosette, resulting from mixed infection by the Sat-RNA causing chlorotic and green mottled variant, has been reported from East Africa [11,6].…”

Section: Introductionmentioning

confidence: 99%

Incidence of Groundnut Rosette Disease (GRD) and Genetic Diversity of Groundnut Rosette Assistor Virus (GRAV) in Western Kenya

Mukoye¹,

Ndong’a²,

Wéré³

2019

IJGG

View full text Add to dashboard Cite

show abstract

“…High significant differences (P < 0.01) was recorded by the locations except LLS and GRD. These differences indicated the presence of high genetic variability in the genotypes (Wambi, et al, 2014 andMugisa et al, 2016) and locations for these traits. The highly significant (P < 0.001) difference of genotype-by-location interactions for all traits except LLS and GRD and significant (P < 0.01) variance due to genotype-by-treatment for RWC and pod weight this confirmed existence of wide variation among genotypes, locations and treatments.…”

Section: Phenotypic Variabilitymentioning

confidence: 99%

Yield Penalty Associated With Stacking Resistance to Late Leaf Spot, Rosette Diseases and Drought Stress in Groundnut (Arachis Hypogaea L.)

Mohammed¹

2019

IJAR

View full text Add to dashboard Cite

“…Rosette disease manifest in two major symptoms; chlorotic (yellow) and green rosette which occur throughout the SSA, and sometimes occur in the same field [26]. A less common third symptom variant, called mosaic rosette, resulting from mixed infection of the groundnut cultivars by the Sat-RNA causing chlorotic and green mottled variant, has been reported from East Africa [16,1].…”

Section: Symptoms Of Grdmentioning

confidence: 99%

Genetic Diversity of Groundnut Rosette Disease Causal Agents Towards Its Management: A Review

Mukoye¹

2019

IJGG

View full text Add to dashboard Cite

In this review, the genetic diversity of the three causal agents of Groundnut Rosette Disease (GRD) in Sub-Saharan Africa (SSA) are discussed. Epidemics of GRD viruses in SSA, often reduce groundnut productivity. The etiology of GRD is a complex, involving three agents; Groundnut rosette assistor luteovirus (GRAV), Groundnut rosette umbravirus (GRV) and a Satellite-RNA (Sat-RNA) of GRV. The complex etiology and lack of sensitive and specific diagnostic tools, are major limitations in understanding the epidemiology of GRD viruses, and developing appropriate management strategies for the disease. Nucleotide identity of 97 to 100% among GRAV isolates from different regions in Kenya have been reported. Sat-RNA sequences from Kenya shared nucleotide identity of 95% with Malawian isolate (M24S) and 89% with Nigerian isolate (NG3a). GRAV CP gene was highly conserved (97-99%) regardless of the geographical distance. However, for GRV and Sat-RNA diversity increased with increase in geographical distance. In addition, phylogenetic analysis showed that isolates of GRV (ORF3 and 4) and Sat-RNA clustered together depending on the country of origin. Recent study has unveiled a chlorotic variant of Sat-RNA in Kenya with 97% sequence identity to the Malawian chlorotic isolate (M24S). Pathogen derived resistance (PDR) suitable for each diverse regions where the disease occurs is a promising management strategy which mainly depends in studies to deeply understand the genetic diversity of the three GRD causal agents. Currently, GRAV-CP is the best candidate for PDR.

show abstract

Determinants of groundnut rosette virus disease occurrence in Uganda

Cited by 9 publications

References 9 publications

Incidence of Groundnut Rosette Disease (GRD) and Genetic Diversity of Groundnut Rosette Assistor Virus (GRAV) in Western Kenya

Incidence of Groundnut Rosette Disease (GRD) and Genetic Diversity of Groundnut Rosette Assistor Virus (GRAV) in Western Kenya

Yield Penalty Associated With Stacking Resistance to Late Leaf Spot, Rosette Diseases and Drought Stress in Groundnut (Arachis Hypogaea L.)

Genetic Diversity of Groundnut Rosette Disease Causal Agents Towards Its Management: A Review

Contact Info

Product

Resources

About