Determinación de la calidad bacteriológica del aire en un laboratorio de microbiología en la Universidad Distrital Francisco José de Caldas en Bogotá, Colombia

Bohórquez, César Alberto Romero; Alvarado, Diego Fernando Castañeda; Peñaloza, Gloria Stella Acosta

doi:10.22490/24629448.1756

Cited by 6 publications

(6 citation statements)

References 13 publications

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“…Para tomar las muestras del aire interno del ambiente de los sitios o áreas de trabajo seleccionados, se aplicó la técnica de sedimentación por gravedad [18,19], utilizando las placas Compact Dry ® para bacterias aerobias [20,21]. Para poder utilizar la técnica de sedimentación por gravedad en los ambientes seleccionados, previamente se prepararon las placas de medio de cultivo Compact Dry ® para bacterias aerobias, agregando un volumen de 1 mL de agua estéril a cada una de las placas a ser utilizadas, dejando transcurrir diez minutos para su solidificación en condiciones de esterilidad.…”

Section: Caracteristicas Generalesunclassified

“…Finalizado el tiempo de incubación, se contaron las colonias crecidas mediante un contador de colonias y se expresaron los resultados como Unidades Formadoras de Colonias por centímetro cuadrado por minuto (UFC/cm 2 /minuto). Posteriormente cada valor obtenido fue transformado a las unidades UFC/m 3 , utilizando la ecuación de Omeliansky [19,22].…”

Section: Caracteristicas Generalesunclassified

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Calidad bacteriológica del aire en ambientes académicos administrativos de la Universidad Central del Ecuador

González,

Chávez,

Araque

et al. 2024

refa

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La contaminación del aire de los ambientes de trabajo es un problema resaltante a nivel mundial, constituyéndose en un fenómeno con incidencia en la salud del trabajador a través de infecciones y enfermedades crónicas, lo cual produce una reducción de la esperanza de vida de las personas, así como pérdidas económicas por el ausentismo laboral que provoca. En este sentido el objetivo de la presente investigación fue cuantificar la microbiota bacteriana del aire interior de unidades académico administrativas de la Universidad Central del Ecuador. El diseño experimental aplicado fue el de una investigación exploratoria, observacional descriptiva. Se recolectaron 304 muestras en 38 sitios seleccionados. Se utilizó como técnica de muestreo la deposición por gravedad utilizando placas Compact Dry® para bacterias aerobias, expuestas por diez minutos en cada uno de los ambientes seleccionados, incubándose posteriormente a 37°C durante un máximo de 7 días. La cuantificación de bacterias aerobias se expresó en unidades formadoras de colonias por metro cubico. Los valores promedios más altos de colonias bacterianas se obtuvieron en los ambientes de las facultades de ciencias administrativas (8,40 x 102), filosofía (8,30 x 102), comunicación social (7,40 x 102) y del hospital del día universitario (7,52 x 102). Los resultados evidencian la presencia de una microbiota bacteriana en el aire interior de la mayoría de las instalaciones de la Universidad Central del Ecuador dentro de los límites establecidos a nivel internacional, lo cual representa un riesgo bajo para la salud de los usuarios.

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Section: Caracteristicas Generalesunclassified

Calidad bacteriológica del aire en ambientes académicos administrativos de la Universidad Central del Ecuador

González,

Chávez,

Araque

et al. 2024

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“…Plate Count Agar (PCA) culture media were used, incubated at 35°C±2°C for 48 hours for the total count of bacteria CFU; and Oxytetracycline Glucose Agar (OGA), incubated at 25°C±2°C for 5 days for total fungi CFU count. (20) After standardization, volumes of 200 L, 500 L, and 1000 L of air from each health environment were sampled. Bacteria were identified by mass spectrometry (MALDITOF, Bruker) and fungi, by biochemical tests (API ID, Biomerieux) (Figure 1).…”

Section: The Studymentioning

confidence: 99%

Evaluation of an ozone generation system for the control of microorganisms in the indoor air of sanitary environments

Yali–Rivera¹,

Rosales-Rimache²

2022

Rev. Cuerpo Med. HNAAA

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Objetive: Evaluate an Ozone Generating System (OGS) through of the microorganisms count and identificated in the air of the sanitary environments of the National Institute of Health (INS) from Peru. The study: These were exposed to OGS for 1 min per m2, air samples were collected at volumes of 200L, 500L and 1000L by impaction, pre and post exposure to OGS, cultures were obtained on Plate Count Agar (PCA) and Oxytetracycline Glucose Agar (OGA) grown at 35°C±2°C for 48 hours and 25°C ± 2°C for 5 days. Findings: A decrease in the count of fungi after exposure to OGS, the difference being statistically significant for the volumes of 200L (p=0.047) and 1000 L (p<0.005); Aspergillus spp. species were identified in most cultures, in bacteria no significant difference was observed. Conclusions: The OGS is a method that inhibits the growth of most fungi in sanitary environments.

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“…The microbiological analysis of the air was conducted using the passive gravity sedimentation method, which consists of viable microorganisms present in the air being transported and deposited onto the surface of a solid culture medium (in a 90 mm diameter Petri dish) by air currents present in the area (Figure 3) [15,19]; Petri dishes were open for 1 h, located at a height of 1 m from the ground and 1 m from walls, and contained agar tryptone-yeast extract for the isolation and quantification of aerobic mesophiles [24], violet red bile lactose agar (RVBA) for coliforms [25], and potato dextrose agar for molds and yeast [26]. The Petri dishes were subsequently incubated at 35 ± 2 • C/48 h for aerobic mesophiles, 35 ± 2 • C/24 h for coliforms, and 25 ± 1 • C/5 d for molds and yeast [19,[24][25][26].…”

Section: Microbiological Analysismentioning

confidence: 99%

Microbiological Analysis of the Air in a Popular Fish Processing and Marketing Area

Quintanilla-Martínez

Aguirre-Güitrón

Espinosa-Chaurand

et al. 2022

Applied Biosciences

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Fish are marketed as a food and consumed worldwide. During the production of food, contamination by microorganisms is possible through the air, soil, water, surfaces, food handlers, etc. The air does not have a natural microbial composition, but it is a vehicle for the transmission of microorganisms of economic and health interest because they are associated with food spoilage and human diseases. The objective of this study was the microbiological analysis of the air in an area popular for the processing and marketing of fish products in the city of Tepic Nayarit. Using the passive or sedimentation method to collect microorganisms present in the air, the proportion of aerobic mesophile bacteria, coliform bacteria, fungi and yeast was determined at different locations in the fish processing and marketing area for four weeks. The results indicated that the aerobic mesophiles had the highest counts among all the microbial groups analyzed at the twelve different sampling points during the four weeks of the study; their numbers ranged from 2.44 to 2.95 log CFU/m3/h, followed by molds with counts from 1.44 to 2.75 log CFU/m3/h, yeasts with counts from 0.7 to 2.01 log CFU/m3/h and coliforms with counts that ranged from 0.7 to 1.68 log CFU/m3/h. We determined the proportion of the viable microbiological population present in the air at the different sampling points of the study area; several of these sampling points presented values above those recommended by various agencies around the world. Knowledge of the biological hazards transported through the air is important to establish and reduce the risk to the health of occupants and the contamination pathways of processed and marketed fishery products that may be associated with spoilage and foodborne diseases.

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Determinación de la calidad bacteriológica del aire en un laboratorio de microbiología en la Universidad Distrital Francisco José de Caldas en Bogotá, Colombia

Cited by 6 publications

References 13 publications

Calidad bacteriológica del aire en ambientes académicos administrativos de la Universidad Central del Ecuador

Calidad bacteriológica del aire en ambientes académicos administrativos de la Universidad Central del Ecuador

Evaluation of an ozone generation system for the control of microorganisms in the indoor air of sanitary environments

Microbiological Analysis of the Air in a Popular Fish Processing and Marketing Area

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