Determinación de anticuerpos totales (IgG/IgM) y específicos (IgM) para el virus de la hepatitis E y detección molecular del virus en heces de humanos con o sin exposición ocupacional a porcinos en 10 municipios de Antioquia

Gutiérrez-Vergara, CC; Rodríguez, Berardo de J; Parra, Jorge Eduardo Duque; Londoño, Guillermo Antonio Correa; López-López, Lucelly; Lopéz-Herrera, Albeiro; Gutiérrez-Builes, Lina A.

doi:10.17533/udea.iatreia.v28n3a03

Cited by 6 publications

(6 citation statements)

References 22 publications

(18 reference statements)

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“…A 10‐year gap in knowledge followed, until 2011, when studies from Argentina, Bolivia, Brazil and Colombia, reported respective HEV prevalences . This wave of studies followed the global reports of chronic hepatitis E virus infection by HEV‐3 in immunosuppressed individuals, especially those with solid organ transplantation as well as the reports of extra‐hepatic manifestations of HEV.…”

Section: Detection Of Hev In Humans In South Americamentioning

confidence: 99%

Hepatitis E virus in South America: The current scenario

Pisano

Martinez‐Wassaf²,

Mirazo

et al. 2018

Liver International

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Hepatitis E virus (HEV) is one of the most frequent causes of acute viral hepatitis of enteric transmission worldwide. In South America the overall epidemiology has been little studied, and the burden of the disease remains largely unknown. A research of all scientific articles about HEV circulation in South America until November 2017 was carried out. Human seroprevalences of HEV varied according to the studied population: blood donors presented prevalence rates ranging from 1.8% to 9.8%, while reports from HIV‐infected individuals, transplant recipients and patients on hemodialysis showed higher prevalence rates. Only 2 cases of chronic hepatitis in solid‐organ transplant patients from Argentina and Brazil have been described. Detection of HEV in the swine population is widely prevalent in the region. Anti‐HEV antibodies have also been recently documented in wild boars from Uruguay. Although scarce, studies focused on environmental and food HEV detection have shown viral presence in these kind of samples, highlighting possible transmission sources of HEV in the continent. HEV genotype 3 was the most frequently detected in the region, with HEV genotype 1 detected only in Venezuela and Uruguay. HEV is widely distributed throughout South America, producing sporadic cases of acute hepatitis, but as a possible agent of chronic hepatitis. Finding the virus in humans, animals, environmental samples and food, show that it can be transmitted through many sources, alerting local governments and health systems to improve diagnosis and for the implementation of preventive measures.

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Section: Detection Of Hev In Humans In South Americamentioning

confidence: 99%

Hepatitis E virus in South America: The current scenario

Pisano

Martinez‐Wassaf²,

Mirazo

et al. 2018

Liver International

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show abstract

“…In Colombia, serological and molecular evidence of HEV infection in patients with clinical diagnosis of viral hepatitis has been demonstrated [ 29 , 30 ], as well in workers from swine farms [ 31 , 32 ]. Besides, serological and molecular evidence of HEV infection was established in fecal and liver samples of swine [ 33 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

First evidence of the Hepatitis E virus in environmental waters in Colombia

et al. 2017

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Hepatitis E virus (HEV) is one of the main causes of acute viral hepatitis of enteric transmission. HEV has been detected in environmental samples in several countries from Europe and Asia, constituting a risk factor for waterborne infection. In Colombia, HEV has been identified in samples obtained from patients as well as from swine, but no environmental studies have been carried out. To determine if HEV is present in environmental waters, samples from the main source of drinking water plant and of wastewater system of eight municipalities and two villages of Antioquia state (North West Colombia), were collected between December 2012 and April 2014. The HEV genome was detected by RT-PCR in 23.3% (7/30) of the samples from the main source of drinking water plants and in 16.7% (5/30) from sewage. Viral concentrates obtained from three positive sewage samples were used to inoculate HepG2 cell cultures that were followed for one month; however, the viral genome was not detected in any cell culture. This study demonstrates the circulation of HEV in both source of drinking water plants and wastewater in Antioquia state, Colombia. The presence of HEV in environmental waters could be a risk for waterborne transmission in this population. The findings of the present study, together with the evidence of HEV circulation in human and swine in Colombia, should be consider by public health authorities for the development of surveillance programs and the inclusion of HEV infection diagnosis in the guidelines of viral hepatitis in the country. This is the first report of HEV in environmental samples in Colombia and the second one in Latin America.

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“…En muestras de pacientes con diagnóstico clínico de hepatitis virales provenientes de 15 de los 32 departamentos del país se describió una frecuencia de 6,3% (16/253) para anti-VHE tipo IgG, mientras que en muestras de pacientes en Medellín la frecuencia de anticuerpos IgG fue de 10,9% (10/91) (11). Por otro lado, en trabajadores de fincas porcícolas de Caldas, San Pedro y Guarne (Antioquia), Betancur y colaboradores reportaron una frecuencia de anticuerpos IgG del 11,2% (11/98) ( 13), similar a la frecuencia de 15,7% (25/159) IgG/IgM descrita por Gutiérrez y colaboradores en un estudio realizado en 10 municipios del departamento de Antioquia (Barbosa, Bello, Caldas, Medellín, Entrerríos, Don Matías, San Pedro, Santa Rosa de Osos, Jericó y Rionegro) en personas con exposición ocupacional a porcinos (14). Estas personas constituyen una población de riesgo para la infección por VHE; sin embargo, en todos los casos la seropositividad fue menor a la encontrada en este estudio.…”

Section: Yarumalunclassified

“…Gutiérrez y colaboradores reportan, además, una seropositividad anti-VHE de 5,9% (2/34) en individuos convivientes con personas con exposición laboral a cerdos, y de 7,2% (71/983) en población general de la zona urbana de dichos municipios (14). Un reporte reciente evaluó la coinfección por el virus de la Hepatitis E y otros agentes etiológicos de hepatitis viral en muestras de suero de pacientes de diferentes regiones del país, y encontró una frecuencia de anticuerpos anti-VHE tipo IgG del 25,3% (342/1097), tipo IgM del 5,6% (126/1097), y, de ambos marcadores, del 5,8% (64/1097) (23).…”

Section: Yarumalunclassified

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Frecuencia de anticuerpos contra el virus de la hepatitis E en donantes de sangre del municipio de Yarumal, Antioquia

Duque

Restrepo

Rojas

et al. 2017

Rev. Colomb. Gastroenterol.

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Introducción: el virus de la Hepatitis E (VHE), transmitido por la ruta fecal-oral, causa enfermedad hepática aguda. En Colombia se han realizado algunos estudios en pacientes con diagnóstico de hepatitis viral, en trabajadores de fincas porcícolas, en población porcina y en muestras ambientales. Objetivo: evaluar la presencia de anticuerpos anti-VHE en muestras de donantes de sangre del municipio de Yarumal, departa- mento de Antioquia. Metodología: se obtuvieron muestras de suero de donantes de sangre colectadas por la Cruz Roja Colombiana en una campaña de donación voluntaria en el municipio de Yarumal. En las muestras se determinó la presencia de anticuerpos anti-VHE tipo IgM e IgG mediante estuche comercial de ELISA. Resultados: se analizaron 42 muestras de suero, 19 de las cuales (45,2%) fueron positivas para anticuerpos anti-VHE IgG. Ninguna de las muestras fue positiva para anticuerpos anti-VHE tipo IgM. Conclusiones: este es el primer reporte de anticuerpos anti-VHE en donantes de sangre en Colombia. La frecuencia de anti-VHE (45,2%) es mayor a lo reportado previamente en otros estudios realizados en el país y a lo reportado en do- nantes de sangre en otros países de América Latina. Esta frecuencia podría estar relacionada con el contacto con cerdos infectados, así como con la exposición a agua contaminada con el virus. Sin embargo, estudios adicionales deben ser realizados en otras poblaciones similares en el país para confirmar este hallazgo.

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Determinación de anticuerpos totales (IgG/IgM) y específicos (IgM) para el virus de la hepatitis E y detección molecular del virus en heces de humanos con o sin exposición ocupacional a porcinos en 10 municipios de Antioquia

Cited by 6 publications

References 22 publications

Hepatitis E virus in South America: The current scenario

Hepatitis E virus in South America: The current scenario

First evidence of the Hepatitis E virus in environmental waters in Colombia

Frecuencia de anticuerpos contra el virus de la hepatitis E en donantes de sangre del municipio de Yarumal, Antioquia

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