DETEKSI KONTAMINAN FRAGMEN DNA PENGKODE cyt b BABI  PADA SAMPEL SOFTGELLCANDY TAK BERLABEL HALAL

Rachmawati, Yuanita; Rokhim, Saiku; Munir, Misbakhul; Agustina, Eva

doi:10.14710/halal.v1i1.3115

Cited by 8 publications

(12 citation statements)

References 2 publications

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“…One of the detection methods often used to test pig components and their derivatives in a product is a DNA-based detection method, namely the PCR (Polymerase Chain Reaction) method (Adzakiyyi et al, 2020). Detect DNA using PCR (Polymerase Chain Reaction) has several types of methods, namely conventional PCR, Multiplex PCR, and RT-PCR (Polymerase Chain Reaction) (Rachmawati et al, 2018). The PCR (Polymerase Chain Reaction) method is a DNA amplification-based method that has higher specifications and sensitivity than other methods (Fadhlurrahman et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

study of pig dna analysis methods in cosmetics: a review

2022

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Collagen is one of the components used for cosmetics. Functionally, collagen is derived from pig fat, which functions as a skin conditioning agent (Emollient), emulsion stabilizer (Emulsifier), and as ingredient to increase the viscosity of cosmetic preparations. The method that can detect pig content is the PCR method, which can confirm the presence of DNA content in pigs. In this study, an analysis of cosmetic products containing porcine DNA was carried out using the PCR method. This research was divided into two stages: the initial stage was DNA extraction, and the second stage was PCR analysis. This research is a study through literature study using the narrative review technique. This study aimed to collect information and examine the analysis method of identifying pig DNA contained in cosmetics to obtain the right and accurate way through a literature approach. From the literature study results, it can be concluded that the best extraction method for the isolation of porcine DNA in cosmetic preparations is the extraction method with the Boom Method, while the DNA analysis used to identify porcine DNA in cosmetics is RT-PCR (Real-time Polymerase Chain Reaction). Conventional PCR is because both methods have their respective advantages. The RT-PCR (Real-time Polymerase Chain Reaction) method can amplify and quantify the number of target DNA molecules. The Conventional PCR Method can form DNA bands based on the amplification value.

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Section: Introductionmentioning

confidence: 99%

study of pig dna analysis methods in cosmetics: a review

2022

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“…To be able to print the circuit in PCR technique, dNTPs are also needed which include dATP (nucleotide based on Adenine), dCTP (cytosine), dGTP (guanine), and dTTP (Thymine) (Muladno, 2010). Rachmawati, Rokhim, Munir, & Agustina (2018) explained that this technology is very possible to ascertain whether a food sample contains pig contaminants, even in small amounts. Yusuf (2010) states that there are three important stages in the PCR process that always repeat in 30-40 cycles and take place quickly, namely Denaturation, Annealing, and Extension.…”

Section: Introductionmentioning

confidence: 99%

Sus sp. DNA Encoding cyt b Gene Detection Test on Meat Grinding Samples Using Conventional PCR

Adzakiyyi

Susilowati

Rokhim

et al. 2020

Indonesian Journal of Halal Research

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Micro-entrepreneurs with basic ingredients of processed meat such as meatball who do not have a meat grinder, generally using meat grinder at the public market. The problem that occurs is that there is no clear regulation from the Government regarding the guarantee of the halal meat grinding in the Regional Company. This needs to be enhanced as a study, considering that the grinding material does not only come from Halal substances. The purpose of this study was to test pig DNA in meat grinding samples at PD Pasar Surya Surabaya City by using the conventional Polymerase Chain Reaction (PCR) method. DNA was isolated from 11 PD Pasar Surya meat grinding samples, then spectrophotometry was performed. Spectrophotometry results showed that all samples have high DNA concentrations. The primer used is the cyt b pig gene encoder. Predenaturation is performed at a temperature of 95°C-5 minutes, denaturation of 95°C-45 seconds, annealing 60°C-30 seconds, extension 72°C-40 seconds, and post extension 72°C-5 minutes. The results of PCR analysis were determined by the emergence of DNA bands of ± 149 bp as markers of pig DNA. The results showed negative on sample or no pig contamination in 11 samples tested. While the pig sample as positive control showed a band of ± 149 bp. These results prove that at 11 points of the location of meat grinding there is no contamination of pig DNA.

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“…Goods and/or services related to food, beverages, drugs, cosmetics, chemical products, biological products, genetic engineering products, and goods that are used or utilized by the community, especially Muslims, who enter, circulate, and trade in Indonesia must be labeled Halal [1]. This is guaranteed by the Republic of Indonesia Law No.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular technology can be used as an accurate alternative solution to authenticate/ ascertain whether a food sample contains pork contaminants, judging from the DNA content [2]. This technology is very possible to ascertain whether a food sample contains porcine contaminants, although in small amounts [1]. This is because pork or wild boar are usually sold with mixed beef or other halal meat, as processed foods such as meatballs, sausages, nuggets using milling machines on the market.…”

Section: Introductionmentioning

confidence: 99%

“…Tanabe, S. et al [6], [7] used PCR technology to amplify pig DNA using specific primers of pig DNA, in the testing of processed food allergen contaminants in Japan. Recent molecular technology to detect the presence of contaminants of pig/wild boar DNA in meat and processed meat products is using Real Time PCR technology [1], [8], [9], [10].…”

Section: Introductionmentioning

confidence: 99%

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Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples

Baihaqi¹,

Rachmawati²,

Rokhim³

et al. 2019

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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Meat mills in Indonesia are not halal certified. Small traders who do not have a food processor facility will choose the public market. This can be done both by traders who sell halal processed food and processed pork. It is necessary to study the content of meat mills in the public market so that it becomes critical evidence of an institution authorized to issue a policy immediately. This study aims to examine the content of porcine DNA in meat mills in Pasar Surya Kota Surabaya using the Real Time PCR (qPCR) method. QPCR amplification was performed using a porcine probe primer with annealing temperature optimization of 60 o C. The amplification curve shows an increase in the curve in the positive control (EPC) with a value of Cq 34.00, while the negative control (NTC) is not amplified. The results of this study show that 2 out of 10 samples experienced an increase in the amplification curve on the FAM indicator, meaning that in the meat grinding sample there was a content of porcine DNA. The two samples are 8 and 9 with Cq values of 38.02 and 39.04, respectively. Market Halal Certification must be done immediately.

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DETEKSI KONTAMINAN FRAGMEN DNA PENGKODE cyt b BABI PADA SAMPEL SOFTGELLCANDY TAK BERLABEL HALAL

Cited by 8 publications

References 2 publications

study of pig dna analysis methods in cosmetics: a review

study of pig dna analysis methods in cosmetics: a review

Sus sp. DNA Encoding cyt b Gene Detection Test on Meat Grinding Samples Using Conventional PCR

Real Time PCR Assays for Detection and Quantification of Porcine DNA in Meat Milling Samples

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