Deteksi Ganoderma secara molekuler pada kebun kelapa sawit  yang diberi perlakuan biofungisida Ganor (Molecular detection of Ganoderma on oil palm plantation treated with Ganor biofungicide)

Minarsih, H.; Widiastuti, Happy; Santoso, Djoko

doi:10.22302/iribb.jur.mp.v1i1.289

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Thus, the optimal annealing temperature for detecting G. boninense using the conven tional method ranges from 54 °C. The PCR results are in line with the study conducted by Minarsih et al (2018), where Ganoderma in oil palm plants can be detected by molecular analysis using PCR with specific Ganoderma DNA primers (Gan 1Gan 2 and ITS 1ITS 4) with certain combinations.…”

Section: Resultssupporting

confidence: 89%

Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

Josephin,

Whulanza,

Rahman

et al. 2024

Indones. J. Biotechnol.

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Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.

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Section: Resultssupporting

confidence: 89%

Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

Josephin,

Whulanza,

Rahman

et al. 2024

Indones. J. Biotechnol.

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“…The results of testing organic fungicide (OF) on TM 15 plants that were attacked by Ganoderma sp. showed that the plants were able to recover, as indicated by root growth and began to open their spear leaves and start flowering and produce fruits (Widiastuti et al, 2018). OFs play a role in suppressing the activity of Ganoderma sp.…”

Section: P R E S Smentioning

confidence: 99%

APPLICATIONS OF ORGANIC FUNGICIDES, ENDOPHYTIC BACTERIA, BIOFUNGICIDES, AND BIOSTIMULANTS IN CURATIVE CONTROL OF Ganoderma

DEWANTARA ERIS

2024

JOPR

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Basal stem rot (BSR) caused by Ganoderma boninense is a major oil palm (OP) disease. However, the effective treatment that is practicable in the field as a recommendation remains elusive. This study aimed to assess the effectiveness of the combinations that consisted of organic fungicide (OF), biofungicide (BF), endophytic bacteria (EN), and biostimulant (BS) applied to 20-year old (TM 20) and 14-year old (TM 14) OP with a mild, moderate, and severe level of infection. Results revealed that TM 20 palm recovery response is slower for all infection levels. The effective treatments for mild infection of TM 20 were BF, EN, and BS, meanwhile, for moderate infection, the treatments included OF, BF, EN, and BS. The effective treatment for both mild and moderate infections for TM 14 was the treatment which comprises BF, EN, and BS. It seems that there is no need for BS in this mature palm for both mild and moderate Ganoderma sp. infection since it has rapid recovery. However, the most effective treatment for severe Ganoderma infection in both OP groups was a complete treatment consisting of OF, BF, EN, and BS. We found that severe BSR symptoms could be reduced by providing the complete treatment several times, both holistically and progressively.

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Deteksi Ganoderma secara molekuler pada kebun kelapa sawit yang diberi perlakuan biofungisida Ganor (Molecular detection of Ganoderma on oil palm plantation treated with Ganor biofungicide)

Abstract: Ganor organic fungicide potentially reduces

Cited by 2 publications

References 5 publications

Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

APPLICATIONS OF ORGANIC FUNGICIDES, ENDOPHYTIC BACTERIA, BIOFUNGICIDES, AND BIOSTIMULANTS IN CURATIVE CONTROL OF Ganoderma

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