Deteksi Cemaran DNA Babi Dengan RT-PCR Pada Sosis Tanpa Logo Halal MUI  Dari Empat Kecamatan di Kabupaten Pandeglang Banten

Maulani, Tuti Rostianti; Hadi, Sutarto; Indriati, Marlinda; Suhaemi, Ahmad

doi:10.32662/gatj.v3i2.1171

Cited by 7 publications

(7 citation statements)

References 4 publications

(5 reference statements)

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“…Amplification stages of the qPCR process using Go Tag qPCR Master Mix (Promega), PCR amplification usually uses 35–40 cycles ( Maulani et al ., 2020 ), but in this study, 40 cycles were used. This is because in cycle 40 there is amplification of the DNA sequence.…”

Section: Discussionmentioning

confidence: 99%

Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia

Kholik,

Sukri,

Riwu

et al. 2024

Open Vet J

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Background: Bacterial identification can be done using various testing techniques. Molecular techniques are often used to research dangerous diseases, an approach using genetic information on the pathogenic agent. The enterohemorrhagic invasive species Escherichia coli 0157:H7 was identified from the feces of working horses on the island of Sumbawa. Another advance in molecular technology is genome amplification with qPCR which is the gold standard for detecting E. coli. Aim: This study aims to detect and identify the invasive species Escherichia coli 0157:H7 using the gene encoding chuA with the qPCR method sourced from horse feces. Methods: Fresh fecal samples from horses on Sumbawa Island were isolated and identified, then continued with molecular examination using the gene encoding chuA using the qPCR method. Results: qPCR testing in this study showed that 6 sample isolates that were positive for Escherichia coli 0157:H7 were detected for the presence of the chuA gene, which is a gene coding for an invasive species of E. coli bacteria. The highest to lowest Cq values and Tm from the qPCR results of the sample isolates were 15.98 (4KJ), 14.90 (19KG), 14.6 (3KJ), 13.77 (20KG), 12.56 (5KGB), 12.20 (6KJ). Tm values are 86.7 (4KJ), 86.69 (3KJ), 86.56 (5KGB), 85.88 (20KGB), 85.81 (19KG), 85.74 (6KJ). Conclusion: Validation, standardization of the development, and modification of qPCR technology must be carried out to harmonize testing throughout to avoid wrong interpretation of the test results so that the determination of actions to eradicate and control diseases originating from animals in the field does not occur.

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Section: Discussionmentioning

confidence: 99%

Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia

Kholik,

Sukri,

Riwu

et al. 2024

Open Vet J

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“…Selain dilakukan menggunakan metode PCR konvensional, deteksi gen cyt b babi dapat dilakukan juga menggunakan metode real-time Polymerase Chain Reaction (real-time PCR), primer CYTB-Full ini dikombinasikan dengan qPCR Master Mix. Salah satu keunggulan metode real-time PCR, DNA yang teramplifikasi dapat diamati secara langsung tanpa perlu dilakukan analisis menggunakan gel elektroforesis [13]. Terdapat beberapa penelitian yang menggunakan metode real-time PCR untuk analisis kandungan babi dalam sampel produk industri ( [13]; [14]; [15]; dan [16]).…”

Section: Pendahuluanunclassified

“…Salah satu keunggulan metode real-time PCR, DNA yang teramplifikasi dapat diamati secara langsung tanpa perlu dilakukan analisis menggunakan gel elektroforesis [13]. Terdapat beberapa penelitian yang menggunakan metode real-time PCR untuk analisis kandungan babi dalam sampel produk industri ( [13]; [14]; [15]; dan [16]). Dalam penelitian [16] metode real-time PCR spesifik terhadap gen cyt b babi, serta memiliki batas deteksi absolut sebesar 0,05 ng/µL.…”

Section: Pendahuluanunclassified

Penentuan Sensitivitas dan Spesifisitas Kit PRIME-CYTO untuk Deteksi Kandungan Babi dengan Metode Polymerase Chain Reaction

Iskandar¹,

Safitri

Setyaningrum³

et al. 2023

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Indonesia merupakan negara dengan mayoritas penduduk beragama Islam. Dalam syariat Islam, terdapat larangan mengkonsumsi produk non-halal, di antaranya produk yang mengandung babi. Gen cytochrome b pada babi terpilih sebagai gen pengkode spesies babi dengan bantuan metode analisis Polymerase Chain Reaction (PCR). Penelitian ini dilakukan untuk mengetahui sensitivitas dan spesifisitas kit PRIME-CYTO (Gabungan antara Green Master Mix dan primer CYTB-Full untuk PCR konvensional; gabungan qPCR Master Mix dan primer CYTB-Full untuk real-time PCR) untuk deteksi kandungan gen cytochrome b (cyt b) babi. Hasil penelitian menunjukkan kit PRIME-CYTO sensitif terhadap gen cyt b babi pada metode PCR konvensional dan real-time PCR. Persentase sensitivitas dan spesifisitas kit dengan metode PCR konvensional berturut-turut adalah 100% dan 100%; pada real-time PCR adalah 85% dan 10%. PCR konvensional dapat mendeteksi isolat DNA daging babi hingga kadar minimal 3 ng/μL; pada isolat DNA sosis babi hingga kadar minimal 10 -7 ng/μL. Penggunaan Master Mix dengan probe disarankan untuk meningkatkan spesifisitas pada metode real-time PCR.

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“…Pencampuran daging babi dalam produk Pangan Asal Hewan merupakan hal yang bertentangan dengan agama Islam karena menjadikan produk tersebut haram untuk dikonsumsi [4]. Hal ini merupakan permasalahan yang penting karena Indonesia adalah negara terbesar dengan penduduk mayoritas muslim di dunia [5].…”

Section: Pendahuluanunclassified

Aplikasi Pembeda Daging Sapi dan Babi dengan Metode Color Moment dan Local Binary Pattern Histogram

Edi,

Pribadi

2023

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Meat is one of the main food ingredients consumed by humans because it contains a lot of high protein, so it can increase intelligence and increase the stamina that humans need to carry out activities of daily life. Due to the very high level of meat consumption, these meats are often found in the market. The price of pork is cheaper than beef. The price difference between the two meats has led to the emergence of fraudulent practices in the beef trade. To solve this problem, an application to distinguish beef and pork can be designed to help socialize to the public about how to distinguish beef and pork. In this study, color characteristics will be used to distinguish pork and beef, because from previous studies the color characteristics have a higher accuracy. The method that can be used to extract features from meat images is the color moment method. Color feature extraction consists of Mean, Standard Deviation, and Skewness features. Meanwhile, to carry out the process of detecting the type of meat, the Local Binary Pattern Histogram (LBPH) method will be used. LBPH is a technique of the Local Binary Pattern (LBP) method to change the performance of object recognition results. The result of this research is an application that can be used to distinguish beef and pork. The process of recognizing the type of meat using the Color Moment and LBPH methods has a high success rate so that the Color Moment and LBPH methods can be applied to detect the type of meat, with a success rate of 99.33%.

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Deteksi Cemaran DNA Babi Dengan RT-PCR Pada Sosis Tanpa Logo Halal MUI Dari Empat Kecamatan di Kabupaten Pandeglang Banten

Cited by 7 publications

References 4 publications

Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia

Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia

Penentuan Sensitivitas dan Spesifisitas Kit PRIME-CYTO untuk Deteksi Kandungan Babi dengan Metode Polymerase Chain Reaction

Aplikasi Pembeda Daging Sapi dan Babi dengan Metode Color Moment dan Local Binary Pattern Histogram

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