Detection Times for Urinary Ethyl Glucuronide and Ethyl Sulfate in Heavy Drinkers during Alcohol Detoxification

Helander, Anders; Böttcher, Michael; Fehr, Christoph; Dahmen, Norbert; Beck, Olof

doi:10.1093/alcalc/agn084

Cited by 195 publications

(169 citation statements)

References 39 publications

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“…As far as we know, there are no further studies testing tartrate excretion beyond the day after its consumption. In any case, the values of the combined model of tartrateEtG of consumers≥24h and <72h are in keeping with the kinetics results of studies presented above (Helander et al, 2009;Stalmach et al, 2013;Weinmann et al, 2004;Wurst et al, 2006).…”

Section: Detection Times Of Biomarkers From the Modelsupporting

confidence: 84%

“…Weinmann et al confirmed by mass spectrometry that after 9 g of ethanol intake (0.16g/kg), EtG reached a maximum concentration at 8-10 h and is detectable over 30 h (Weinmann et al, 2004), this fact is in line with our results. Similarly other studies reported detection up to 48h (~0.5 g/kg) (Wojcik and Hawthorne, 2007)and up to 130h (~5days) in extreme cases of alcohol intoxication (Helander et al, 2009). Tartrate is reported excreted over 24h after acute intake of grape juice (Stalmach et al, 2013).…”

Section: Detection Times Of Biomarkers From the Modelmentioning

confidence: 51%

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An NMR metabolomics approach reveals a combined-biomarkers model in a wine interventional trial with validation in free-living individuals of the PREDIMED study

et al. 2014

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The development of robust biomarkers of consumption would improve the classification of participants with regard to their dietary exposure. In addition, validation of them in free-living individuals remains an important challenge. The aim of this study is to assess wine intake biomarkers using an NMR metabolomic approach to measure the utility of these biomarkers in a wine interventional study (WIS, n=56) and also to evaluate them in a free-living individuals (PREDIMED study, n=91). Nine metabolites showed a significantly higher presence in urinary excretion in the WIS after wine intake: five food metabolome metabolites (tartrate, ethyl glucuronide [EtG], 2,3-butanediol, mannitol, and ethanol); one related to the endogenous response to wine exposure (3-methyl-2-oxovalerate) and three unidentified compounds. Receiver operating characteristic (ROC) curve for each single metabolite were evaluated and exhibited areas under the curves (AUC) between 67.4% -86.3% when they were evaluated individually. Then, a logistic regression model was fitted to generate a combined-biomarkers model using these metabolites. The model generated which included tartrate-EtG, showed an AUC of 90.7% in WIS. Similarly, the AUC in the PREDIMED study, the biomarker model was 92.4%. Results showed that a model combining tartrate-EtG is more useful for evaluating exposure to wine than single biomarkers, both in interventional studies and epidemiological data. To our knowledge, this is the first time that a combined-biomarker model using an NMR platform in wine biomarkers' research has been generated and reproduced in a free-living population.2

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Section: Detection Times Of Biomarkers From the Modelsupporting

confidence: 84%

Section: Detection Times Of Biomarkers From the Modelmentioning

confidence: 51%

An NMR metabolomics approach reveals a combined-biomarkers model in a wine interventional trial with validation in free-living individuals of the PREDIMED study

et al. 2014

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“…For the analysis of EtG in urine and serum, methods based on GC-MS [14 -16], LC-MS [1, 2, 5, 12, 15 -19] and ELISA [20,21] have been developed. Furthermore, CZE [22,23] and ITP-CZE [24] were applied to the determination of EtG in serum, and a commercial enzyme immunoassay (EIA) for urinary EtG became recently available and was found to be suitable for rapid urine screening [3,25,26]. The literature suggests that the calibration range for EtG in serum has to be in the low mg/L range.…”

Section: Introductionmentioning

confidence: 99%

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Jung

Caslavska

Thormann

2009

J of Separation Science

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Ethyl glucuronide (EtG) is a marker of recent alcohol consumption. For the optimization of the analysis of EtG by CZE with indirect absorbance detection, the use of capillaries with permanent and dynamic wall coatings, the composition of the BGE, and various sample preparation procedures, including dilution with water, ultrafiltration, protein precipitation, and SPE, were investigated. Two validated screening assays for the determination of EtG in human serum, a CZE-based approach and an enzyme immunoassay (EIA), are described. The CZE assay uses a coated capillary, 2,4-dimethylglutaric acid as an internal standard, and a pH 4.65 BGE comprising 9 mM nicotinic acid, epsilon-aminocaproic acid and 10% v/v ACN. Proteins are removed via precipitation with ACN prior to analysis and the LOQ is 0.50 mg/L. The EIA is based upon commercial reagents which are promoted for the determination of urinary EtG. Krebs-Ringer solution containing 5% BSA is used as a calibration matrix. All samples are ultrafiltered prior to analysis of the ultrafiltrate on a Mira Plus analyzer. Assay calibration ranged between 0 and 2 mg/L and the upper reference limit was determined to be 0.05 mg/L. Both assays proved to be suitable for the analysis of samples from different individuals. For EtG levels above 0.50 mg/L, good agreement was observed for the comparison of the results of the two methods.

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“…The conjugated minor ethanol metabolites ethyl glucuronide (2 ) and ethyl sulfate (3 ) and fatty acid ethyl esters (4 ) are examples of such tests. Ethyl glucuronide and ethyl sulfate are excreted in urine for a much longer time than ethanol and hence have gained popularity as diagnostically sensitive and specific tests to spot recent alcohol consumption (5)(6)(7). For detection and follow-up of long-term risky or heavy drinking, measurement of the alcohol-related change in the serum transferrin glycoform profile known as carbohydrate-deficient transferrin (CDT) 2 (8, 9 ) has become a standard method, often used in combination with liver function tests (e.g., ␥-glutamyltransferase) (1 ).…”

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confidence: 99%

Molecular Species of the Alcohol Biomarker Phosphatidylethanol in Human Blood Measured by LC-MS

2009

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BACKGROUND:The alcohol biomarker phosphatidylethanol (PEth) comprises a group of ethanolderived phospholipids formed from phosphatidylcholine by phospholipase D. The PEth molecular species have a common phosphoethanol head group onto which 2 fatty acid moieties are attached. We developed an electrospray ionization (ESI) LC-MS method for qualitative and quantitative measurement of different PEth species in human blood.

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Detection Times for Urinary Ethyl Glucuronide and Ethyl Sulfate in Heavy Drinkers during Alcohol Detoxification

Abstract: During alcohol detoxification, EtG and EtS remained detectable in urine for several days. The detection times showed wide inter-individual variations, also after adjusting values for urine dilution and to the estimated times for a completed ethanol elimination.

Cited by 195 publications

References 39 publications

An NMR metabolomics approach reveals a combined-biomarkers model in a wine interventional trial with validation in free-living individuals of the PREDIMED study

An NMR metabolomics approach reveals a combined-biomarkers model in a wine interventional trial with validation in free-living individuals of the PREDIMED study

Determination of ethyl glucuronide in human serum by capillary zone electrophoresis and an immunoassay

Molecular Species of the Alcohol Biomarker Phosphatidylethanol in Human Blood Measured by LC-MS

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