Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum

Elsayed, Abdelrahman H.; Enan, Gamal; Al-Mohammadi, Abdul-Raouf; Moustafa, A. H.; El‐Gazzar, Nashwa

doi:10.3390/molecules25204822

Cited by 13 publications

(12 citation statements)

References 49 publications

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“…All the experiments were conducted in triplicate and the results were expressed using one-way ANOVA analysis for estimating means and standard deviations (±SD) [64]. The test was followed by the least significant difference (LSD) test with statistical WASP software version 2.0; LSD, at significant level (p<0.05).Sample symbols (a.a): Mean nonsignificant difference; (a.b): Mean significant difference [61,65].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Peptides Produced by Alcalase from Cowpea Seed Proteins

et al. 2021

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Cowpea seed protein hydrolysates (CPH) were output from cowpea seeds applying alcalase® from Bacillus licheniformis. CPH with an elevated level of hydrolysis was fractionated by size exclusion chromatography (SEC). Both CPH and SEC-portions showed to contain antimicrobial peptides (AMPs) as they inhibited both Gram-positive bacteria, such as Listeria monocytogenes LMG10470 (L. monocytogenes), Listeria innocua. LMG11387 (L. innocua), Staphylococcus aureus ATCC25923 (S.aureus), and Streptococcus pyogenes ATCC19615 (St.pyogenes), and Gram-negative bacteria, such as Klebsiella pnemoniae ATCC43816 (K. pnemoniae), Pseudomonas aeroginosa ATCC26853 (P. aeroginosa), Escherichia coli ATCC25468) (E.coli) and Salmonella typhimurium ATCC14028 (S. typhimurium).The data exhibited that both CPH and size exclusion chromatography-fraction 1 (SEC-F1) showed high antibacterial efficiency versus almost all the assessed bacteria. The MIC of the AMPs within SEC-F1 and CPHs were (25 µg/mL) against P. aeruginosa, E.coli and St. pyogenes. However, higher MICsof approximately 100–150 µg/mL showed for both CPHs and SEC-F1 against both S. aureus and L. innocua; it was 50 µg/mL of CPH against S.aureus. The Electro-spray-ionization-mass-spectrometry (ESI-MS) of fraction (1) revealed 10 dipeptides with a molecular masses arranged from 184 Da to 364 Da and one Penta peptide with a molecular mass of approximately 659 Da inthe case of positive ions. While the negative ions showed 4 dipeptides with the molecular masses that arranged from 330 Da to 373 Da. Transmission electron microscope (TEM) demonstrated that the SEC-F1 induced changes in the bacterial cells affected. Thus, the results suggested that the hydrolysis of cowpea seed proteins by Alcalase is an uncomplicated appliance to intensify its antibacterial efficiency.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Peptides Produced by Alcalase from Cowpea Seed Proteins

et al. 2021

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“…The ion source temperature was set at 200 °C. Compounds were identified by comparison of their retention times and mass spectra with those of WILEY 09 and the National Institute of Standards and Technology 2011 mass spectral library (NIST 11) [ 59 , 60 ].…”

Section: Methodsmentioning

confidence: 99%

Chemical Constitution and Antimicrobial Activity of Kombucha Fermented Beverage

Al-Mohammadi¹,

Ismaiel

Ibrahim

et al. 2021

Molecules

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Kombucha is a traditional beverage of sweetened black tea fermented with a symbiotic association of acetic acid bacteria and yeasts. In this study, kombucha fermented beverage (KFB) appeared to include nine chemical groups (alcohols, acids, lactones, condensed heterocyclic compounds, antibiotics, esters, aldehydes, fatty acids, and alkaloids) of many bioactive metabolites, as elucidated by gas chromatography–mass spectrometry (GC-MS) and IR spectra. The fermented metabolic components of KFB seem collectively to act in a synergistic action giving rise to the antimicrobial activity. Four types of kombucha preparations (fermented, neutralized, heat-treated and unfermented) were demonstrated with respect to their antimicrobial activity against some pathogenic bacterial and fungal strains using agar well diffusion assay. KFB exerted the strongest antimicrobial activities when compared with neutralized and heat-treated kombucha beverages (NKB and HKB). Staphylococcus aureus ATCC6538 (S. aureus) and Escherichia coli ATCC11229 (E. coli) were the organisms most susceptible to the antimicrobial activity of kombucha beverage preparations. Finally, the KFB preparation showed remarkable inhibitory activity against S. aureus and E. coli bacteria in a brain heart infusion broth and in some Egyptian fruit juices (apple, guava, strawberry, and tomato). These data reveal that kombucha is not only a prophylactic agent, but also appears to be promising as a safe alternative biopreservative, offering protection against pathogenic bacteria and fungi.

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“…The characterization of functional groups on the surface of nanoparticles was performed by FTIR (Thermo Nicolet model 6700 spectrum Located at Micro-Analytical Center, Cairo University, Giza, Egypt) by employing KBr pellet technique and the spectra were scanned in the 400–4000 cm −1 range at a resolution of 4 cm −1 ( Enan et al, 2020 , El-Sayed et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Assessment the using of silica nanoparticles (SiO2NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)

El‐Gazzar

Almanaa

Reda

et al. 2021

Saudi Journal of Biological Sciences

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Rice husks (RHs) was used as a substrate for biosynthesis of high-value Silica nanoparticles (SiO 2 NPs). An isolate of Trichoderma harzianum MF780864 ( T. harzianum ) was isolated and identified based on the Internal Transcribed Spacers (ITS) sequences; it showed the potentiality to induce SiO 2 NPs in the process of RHs biotransformation. SiO 2 NPs were produced extracellularly and their size was of about 89 nm. SiO 2 NPs characterized by oval, rod and cubical particles by using Transmission Electron Microscope (TEM).The Fourier transform infrared spectroscopy (FTIR) confirmed the presence of various functional groups of biomolecules and capping protein, encapsulating SiO 2 NPs. Water and fish samples were collected from private fish farms in El-Sharkia Governorate, Egypt. Lead (Pb) was detected from water and fish samples at its highest concentration at about 0.088 mg/L. The adsorption capacity of Pb by SiO 2 NPs was evaluated by testing different concentrations of SiO 2 NPs viz. 1, 2, and 3 mg/L, wherein 1 mg/L revealed the highest Pb adsorption efficiency. Within laboratory trials, the results indicated that highest Pb adsorption efficiency revealed through the increasing of SiO 2 NPs concentrations until 120 h. In vivo trial that lasted for 8 weeks, Nile tilapia ( Oreochromis niloticus) (29.78 ± 0.36 g body weight) supplemented with 0.088 mg/L Pb was divided into four experimental groups having three replicates (15 fish/replicate; 45 fish/group). The results showed that SiO 2 NPs supplementation through water revealed significant increase in growth and hematological parameters of O. niloticus . Moreover, enhancement of antioxidant capacity (TAC), and immune related gene expression of IL-1 β were increased in the presence of SiO 2 NPs compared with the groups of Pb exposure. Moreover, Pb residue level in fish muscles was noticeably decreased in the SiO 2 NPs treated groups. Thus, this research opens up other possibilities in the field of using SiO 2 NPs as a lead adsorbent for water bioremediation.

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Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum

Cited by 13 publications

References 49 publications

Antibacterial Peptides Produced by Alcalase from Cowpea Seed Proteins

Antibacterial Peptides Produced by Alcalase from Cowpea Seed Proteins

Chemical Constitution and Antimicrobial Activity of Kombucha Fermented Beverage

Assessment the using of silica nanoparticles (SiO2NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)

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