Detection probability of Campylobacter

Evers, Eric G.; Post, J.; Putirulan, F.F.; Wal, F.J. van der

doi:10.1016/j.foodcont.2009.06.004

Cited by 7 publications

(4 citation statements)

References 6 publications

(4 reference statements)

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“…The difference from the system studied by Evers et al (18) is that Salmonella detection involves a preenrichment step. In our model this is accounted for by the introduction of a material-specific constant (r m ).…”

Section: Discussionmentioning

confidence: 93%

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Koyuncu

Andersson

Häggblom

2010

Appl Environ Microbiol

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The present study compared the performance of commercial PCR-based Salmonella enterica detection methods (BAX System Q7, the iQ-Check Salmonella II kit, and the TaqMan Salmonella enterica detection kit) with culturebased methods (modified semisolid Rappaport-Vassiliadis [MSRV] and NMKL71) in spiked and naturally contaminated samples of feed mill scrapings (FMS), palm kernel meal (PKM), pelleted feed (PF), rape seed meal (RSM), soybean meal (SM), and wheat grain (WG). When results from the various feeds were compared, the number of Salmonella enterica CFU/25 g required to produce a positive were as follows: PKM > FMS ‫؍‬ WG > RSM ‫؍‬ SM ‫؍‬ PF. These data are similar to those developed in earlier studies with culture-based Salmonella detection methods. PCR-based methods were performed similarly to culture-based methods, with respect to sensitivity and specificity. However, many PCR positives could not be confirmed by Salmonella isolation and for that reason the evaluated methods were found to be suitable only when rapid results were paramount. Nevertheless, PCR-based methods cannot presently replace culture-based methods when typing information is required for tracing studies or epidemiological investigations. The observed difference in detection levels is a potential problem when prevalence data are compared as well as when feed ingredients are tested for conformance with microbiological criteria. This paper also presents a statistical model that describes the detection probability when different levels (CFU) of Salmonella contamination are present in feed materials.

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Section: Discussionmentioning

confidence: 93%

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Koyuncu

Andersson

Häggblom

2010

Appl Environ Microbiol

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“…A universal definition of the LOD for microbiological purposes has not yet been established (see e.g., Duarte et al 2015, Evers et al 2010. One popular operational definition in dilution series used by microbiologists is to define the LOD as 1 colony forming unit (CFU) for bacteria or algae, or 1 plaque forming unit (PFU) for viruses (e.g., see Evers et al 2010, Magnani 2021, Sutton 2011. We show that this definition may be too simplistic because it is not associated with any measure of statistical uncertainty (e.g., a confidence level).…”

Section: Introductionmentioning

confidence: 99%

“…One popular operational definition used by microbiologists is to define the LOD in the volume plated as 𝐿 plate = 1 CFU (seeEvers et al 2010, Magnani 2021, Sutton 2011) which, as pointed out above, is different than the recommended ranges for determining the number of colonies to count on a plate (e.g.,30-300;Ben-David and Davidson 2014). Under the Poisson model, this corresponds to 𝛽 = 0.37 (Table…”

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confidence: 99%

Calculating the limit of detection for a dilution series

Sharp

Parker

Hamilton

2023

Journal of Microbiological Methods

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“…The replicates were then scored as either detectable (positive) or nondetectable (negative). In order to determine the sensitivity of the modified magnetic capture qPCR method, the oocyst-count dependent detection probability (Evers et al, 2010) was estimated by using a probit analysis in R (R Development Core Team, 2006) (supplementary material A). The probit model (Equation 2) assumes that the probability of detection is related to the log 10-transformed concentration data as the cumulative normal distribution.…”

Section: Data Analysis 2231 Detection Probability Of the Modified Magnetic Capture Qpcrmentioning

confidence: 99%

Source attribution of human toxoplasmosis

Deng¹

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Chapter 1General introduction PART I: MULTI-HOST TRANSMISSION Chapter 2 Mathematical modelling of Toxoplasma gondii transmission: A systematic review PART II: EPIDEMIOLOGICAL INSIGHTS Chapter 3 Risk factors related to Toxoplasma gondii seroprevalence in indoor-housed Dutch dairy goats Chapter 4 Seroprevalence of Toxoplasma gondii in pregnant women and livestock in the mainland of China: a systematic review and hierarchical meta-analysis PART III: QUANTITATIVE MICROBIAL RISK ASSESSMENTS Chapter 5 The effect of salting on T. gondii viability evaluated and implemented in quantitative risk assessment of meat-borne Toxoplasma gondii infection Chapter 6 Quantitative risk assessment of meat-borne Toxoplasma gondii infection in the mainland of China Chapter 7 Digging into Toxoplasma gondii infections via soil: a quantitative microbial risk assessment approach Chapter 8 General discussion ADDENDUM Summary (in English and Dutch) List of publications Acknowledgements About the author Chapter General introduction GENERAL INTRODUCTION • 13TOXOPLASMOSIS T. gondii infection has a worldwide distribution, and it is generally assumed that one third of the world's human population is infected. The prevalence of infection varies widely between countries and often within a given country . In immunocompetent individuals, most infections are asymptomatic, because the immune system usually keeps the parasites from causing illness. Occasionally, some people may experience flu-like symptoms (e.g., fever, muscle aches, etc.) and swollen lymph nodes for a few weeks or months (Halonen and Weiss, 2013). It was recognised that postnatally acquired T. gondii infection in immune-competent people can cause chorioretinitis (ocular toxoplasmosis) (Gilbert and Stanford, 2000). However, severe consequences are more common in two main risk groups: pregnant women and immuno-compromised individuals .Generally, if women acquire T. gondii infection before pregnancy, the unborn child will be protected, because the mother has developed immunity. However, if primary infection occurs during or just before pregnancy, congenital infection may take place in about 30% of cases . The probability of vertical transmission increases with gestational age, but the consequences for the offspring are more severe in early stages of pregnancy (e.g., major abnormalities in the brain and eye tissues or abortion) (Thiebaut et al., 2007). Infected children who are healthy at birth may develop chorioretinitis later in life.

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Detection probability of Campylobacter

Cited by 7 publications

References 6 publications

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

Calculating the limit of detection for a dilution series

Source attribution of human toxoplasmosis

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