Detection of β-thalassemia by a DNA piezoelectric biosensor coupled with polymerase chain reaction

Minunni, Maria; Tombelli, Sara; Scielzi, Rosalia; Mannelli, Ilaria; Mascini, Marco; Gaudiano, Carlo

doi:10.1016/s0003-2670(03)00065-5

Cited by 56 publications

(30 citation statements)

References 23 publications

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“…To resolve this problem, PCR was used to amplify target DNA. Based on amplification by PCR, DNA QCM biosensor has been successfully applied to determine ␤-thalassemia (Minunni et al, 2003) and apolipoprotein E polymorphisms (Tombelli et al, 2000). But the use of PCR can be laborious and time consuming and requires a number of manipulations.…”

Section: Discussionmentioning

confidence: 99%

Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor

Chen

Zhu

Tang

et al. 2008

Biosensors and Bioelectronics

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Section: Discussionmentioning

confidence: 99%

Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor

Chen

Zhu

Tang

et al. 2008

Biosensors and Bioelectronics

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“…DNA isolated from blood was widely used inforensics 1 ; diseases diagnosis 2,3 ; mutation and polymorphism identifications 4,5 ; neonatal methylation study 6 ; and pathogens detection in human and livestock 3,7,8 . In hematology, DNA isolated from blood was used in diagnosis of leukemia 9 , thalassemia 10 , polycythemia 11 and G6PD deficiency 12 . Blood provide high yield and high quality of DNA as compared to other sources such as saliva, hair, Guthrie cards, and buccalcells 13,14 .…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Comparison of DNA Extraction Using Fresh and Stored Bloods in Molecular Hematology Diagnostic Study

Hassan

Mohamad

Hassan

et al. 2018

Bangladesh J Med Sci

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Abstract:Objective: Blood is the main source of DNA in molecular biology. It provides a high DNA quality and quantity. In this study, we compare the quality and quantity of DNA isolated from stored blood that has been kept at -40°C for one-year to that of fresh blood. Materials and Methods: Twelve fresh and stored blood samples were randomly selected for this study. Nucleo Spin ® Blood L kit was used to isolate the DNA from the samples. The integrity and intensity of DNA were examined through 1.6% agarose gel precast with SYBR ® safe DNA stain. The DNA samples were further examined through PCR amplification and Sanger sequencing. Results: There was no significant difference in quality and quantity of isolated DNA from fresh blood and stored blood samples. The high intensity of an intact DNA band as well as the success in PCR amplification and sequencing are indicators of high DNA quality. Conclusion: Proper storage of patients'left-over whole blood sample at -40°C offers an acceptable alternative for DNA resources in molecular study.

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“…The next component is the transducer, which serves to transform the biochemical signal originating from the recognition element and the target analyte to a quantifi able output. Leading transduction methods include electrochemical, piezoelectrical (Minunni et al 2003 ;Tombelli et al 2009 ), optical (Wandemur et al 2014 ;Khan and Park 2014 ) and calorimetric (Bhand et al 2010 ;Zhang and Tadigadapa 2004 ). The last component is an amplifi cation module that allows the acquired signal to be visualized on a digital display.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotubes: Advances, Integration and Applications to Printable Electrode-Based Biosensors

Hung

Kerman

2015

Nanobiosensors and Nanobioanalyses

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The development of novel analytical techniques for biosensor application has been an area of intense research efforts in the past decade and, in a fi eld where the demand for rapid and cost-effective analyses is high, the use of screen-printed electrodes as opposed to conventional macroelectrodes can effectively satisfy these demands. With advancements in nanotechnology, the use of nanomaterials in biosensors has also added another level of customizability and sensitivity. In this chapter, we described the use of carbon nanotubes in printable electrode-based biosensors for label-free detection approaches for the detection of environmental contaminants and biologically relevant substrates. A discussion of past developments and recent advancements will highlight the broad range of experimental designs suitable for biosensing applications. With continual innovation in this fi eld, it is expected that screen-printing technology will come to the fore in biosensing technology and progress successfully to overcome fundamental challenges in the biosensor fi eld.

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Detection of β-thalassemia by a DNA piezoelectric biosensor coupled with polymerase chain reaction

Cited by 56 publications

References 23 publications

Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor

Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor

A Comprehensive Comparison of DNA Extraction Using Fresh and Stored Bloods in Molecular Hematology Diagnostic Study

Carbon Nanotubes: Advances, Integration and Applications to Printable Electrode-Based Biosensors

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