Detection of wolfgram W1 protein, myelin basic proteins and proteolipids in cultured oligodendrocytes by the electro-immunoblotting method

Nussbaum, J.L.; Nussbaum, F.; Gensburger, C.; Monteros, M.T.Araceli Espinosa De Los; Roussel, G.; Labourdette, G.

doi:10.1016/0304-3940(83)90288-4

Cited by 13 publications

(5 citation statements)

References 24 publications

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“…As reported previously, besides PLP, the antiserum to PLP revealed DM-20 [12,14] and minor high molecular weight bands [13] which could represent stable aggregates of PLP [4], The radioactivity incorporated into PLP and PLP-related polypeptides after immuno précipitation and gel electrophoresis ( fig. 1) was 0.2% of the total TCA-precipitated ra dioactivity measured for both control and mid optic nerves.…”

Section: Resultsmentioning

confidence: 92%

Intrinsic Myelin Proteins Are Normally Synthesized in vitro in the Myelin-Deficient <i>(mld)</i>Mutant Mouse

1983

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The synthesis of myelin basic protein is severely reduced in the central and peripheral nervous system of myelin-deficient (mld) mutant mice. Using an in vitro system and immunoprecipitation, we found a normal rate of synthesis in mld mice for two intrinsic myelin proteins: proteolipid protein in central nervous system myelin and P₀ in peripheral nervous system myelin. These results indicate that protein synthesis on membrane-bound ribosomes is not affected by the mutation.

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Section: Resultsmentioning

confidence: 92%

Intrinsic Myelin Proteins Are Normally Synthesized in vitro in the Myelin-Deficient <i>(mld)</i>Mutant Mouse

1983

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“…In contrast, rat oligodendrocytes in vitro produce differentiation-associated products even in the absence of neurites Roussel et al, 1983;Bologa et al, 1983;Zurbriggen et al, 1984). Recently, Dubois-Dalcq et al (1986) have reported that myelin-associated glycoprotein (MAG), MBP, and proteolipid protein (PLP) are first detected, by immunocytochemistry, in the cytoplasm of the oligodendrocytes in culture, and with time in vitro the staining appeared in the processes or on the surface of the cells, indicating that the oligodendrocytes can go through biochemical differentiation without a continuing signaling from neurons.…”

Section: Expression Of Myelin Components In Mouse Schwa"mentioning

confidence: 99%

Expression of Myelin Components in Mouse Schwann Cells in Culture

Burroni¹,

Fv²,

Ceccarini³

et al. 1988

Journal of Neurochemistry

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Mouse Schwann cells cultured in vitro are capable of expressing basal levels of the major myelin components P1, P2, P0, and galactocerebroside. Numerical counts of immunostained cultures indicated that between 22 and 40% of the cells are positive up to 21 days for all of the components indicated. Electrophoretic analysis of Schwann cells labeled with a 14C-amino acid mixture revealed the presence of proteins with relative mobilities identical to those of P0 and P1. Positive identification of the two proteins was indicated by immunoprecipitation of P1 and immunoblotting of P0. These data show that in the absence of neurites, Schwann cells in culture can express low levels of myelin characteristic components even in the absence of myelin assembly.

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“…However, the proportion of astrocytes (GFAP+ cells) in our mouse brain cell cul tures was still very low at 5 DIV, but reached an average of 60± 18% (average ±SD) by 20 DIV, the other cells (GFAP ) were of mes enchymal origin [51] or oligodendrocytes [40,45], The oligodendrocytes were further iden tified using markers such as gaiactosylceramides (galaçtocerebrosides). The stain ing of cultures with the anti-GC serum re vealed the presence of some GC+ cells al ready in 7-day-old cultures.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Cellular Development and Myelin Production in Primary Cultures of Embryonic Mouse Brain

Fabre¹,

Langley

Bologa

et al. 1985

Dev Neurosci

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The development of cell cultures from embryonic mouse cerebral hemispheres has been followed in detail for periods up to 40 days in culture using a variety of approaches. Functionally well differentiated neurones (shown by receptor binding studies, immunocytochemistry and morphological examination) were found to be abundant early in culture and to form cell contacts with oligodendrocytes characterized both immunocytochemically and morphologically. Myelin-like membranes with the periodicity of classical myelin elaborated by oligodendrocytes were detected only after 30 days in culture when neurones were no longer present. These results are discussed with regard to possible mechanisms of initiation of myelin synthesis.

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Detection of wolfgram W1 protein, myelin basic proteins and proteolipids in cultured oligodendrocytes by the electro-immunoblotting method

Cited by 13 publications

References 24 publications

Intrinsic Myelin Proteins Are Normally Synthesized in vitro in the Myelin-Deficient <i>(mld)</i>Mutant Mouse

Intrinsic Myelin Proteins Are Normally Synthesized in vitro in the Myelin-Deficient <i>(mld)</i>Mutant Mouse

Expression of Myelin Components in Mouse Schwann Cells in Culture

Cellular Development and Myelin Production in Primary Cultures of Embryonic Mouse Brain

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