Detection of white spot baculovirus (WSBV) in giant freshwater prawn, Macrobrachium rosenbergii, using polymerase chain reaction

Peng, Shao-En; Lo, Chu Fang; Ho, Chun Han; Chang, Chin-I; Kou, Guang Hsiung

doi:10.1016/s0044-8486(98)00191-4

Cited by 80 publications

(47 citation statements)

References 12 publications

(13 reference statements)

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“…We tried several different primer pairs targeted on various WSSV ORFs, but only the PCR primers specific to ORF 144 gave a satisfactory detection. Our previous studies (data not shown) showed that the primers (144F/144R) corresponding to ORF 144 of WSSV exhibited a comparable sensitivity to that of Primers 146F1/146R1 and 146F2/146R2 described in a 2-step PCR (Lo et al 1996b, Peng et al 1998. It is easier to perform a 1-step PCR than a 2-step PCR.…”

Section: Discussionmentioning

confidence: 91%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

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White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes -GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.

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Section: Discussionmentioning

confidence: 91%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

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“…), palaemonidian pest shrimp, krill (Acetes sp. ), planktonic copepods and pupae of an ephydridian insect , Kanchanaphum et al 1998, Peng et al 1998, Supamattaya et al 1998, Y. C. Wang et al 1998, Q. Wang et al 2000.…”

mentioning

confidence: 99%

“…The virus can be detected by the use of polymerase chain reaction (PCR), in situ hybridisation or monoclonal antibody assays (Kanchanaphum et al 1998, Peng et al 1998, Poulos et al 2001. Thus, in recent years molecular probes have been developed as diagnostic tools and these are already being used for pathogen detection in commercial shrimp farms.…”

mentioning

confidence: 99%

Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus leniusculus

Jiravanichpaisal¹,

Bangyeekhun²,

Söderhäll³

et al. 2001

Dis. Aquat. Org.

113

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The signal freshwater crayfish Pacifastacus leniusculus was found to be susceptible to infection with white spot syndrome virus (WSSV). Histopathological observations of various tissues of virus-injected crayfish showed similar symptoms to those from WSSV-infected penaeid shrimp, but no appearance of white spots on the cuticle or reddish body colour were observed, although these are the prominent gross signs of white spot disease in shrimp. A gene probe for detecting WSSV was developed in order to detect the virus in affected cells and tissues using in situ hybridisation. Strong signals were observed in cells of virus-injected crayfish, but not in control-injected crayfish. The number of granular haemocytes in virus-injected crayfish was significantly higher than in sham-injected and non-injected crayfish from Days 5 to 8 (p ≤ 0.05) and Days 3 to 8 (p < 0.01) postinjection, respectively. The proportion of granular haemocytes in virus-injected crayfish was also significantly higher than in sham-injected controls from Days 3 to 8 (p < 0.01). These results indicate that WSSV has a significant effect on the proportion of different haemocyte types in the freshwater crayfish.

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“…P. japonicus (kuruma shrimp), and Metapenaeus ensis (sand shrimp). However, in diseased P. penicillatus (red tail shrimp) and Macrobrachium rosenbergii (the giant freshwater prawn), usually the white spots can be seen only after the carapace has been removed, while in portunid crabs such as Scylla serrata, the gross signs of WSS take the unusual form of clouded areas in the last two segments of the fifth pereiopod (Lo et al, 1996a;Peng et al, 1998). The presence of WSSV has been confirmed in wild-caught shrimp (P. monodon, P. japonicus, P .…”

Section: Diagnostic Tools For Wssmentioning

confidence: 99%

Virus-associated White Spot Syndrome of Shrimp in Taiwan: A Review.

Kou

1998

Fish Pathol.

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White spot syndrome (WSS) is a viral disease which affects most of the commercially cultivated marine shrimp species, not just in Taiwan but globally. The principal clinical sign of WSS is the presence of white spots on the proximal surface of the cuticle of the diseased shrimp. The causative agent is a dsDNA virus which consists of an enveloped, rod-shaped nucleocapsid. White spot syndrome virus (WSSV) infection is character ized by rapid disease onset and high mortality. The mortality typically reaches 90% within 2 to 7 days after the onset of the disease. Recently, WSSV was also found in the form of a latent infection in freshly caught wild shrimp and crabs. Under stressful conditions, WSSV was triggered to replicate rapidly and subsequently caused the death of these animals. Using polymerase chain reaction (PCR), we successfully detected WSSV in cultured and captured shrimp and crabs. Detection of WSSV in non-cultured arthropods collected from WSSV-affected shrimp farms revealed that copepods, the pest crab Helice tridens, small pest palaemonid prawns, and possibly the larvae of an ephydrian insect were reservoir hosts of WSSV. The tissues targeted by WSSV originate from both the ectoderm and the mesoderm. In situ hybridization and transmission electron microscopy (TEM) showed evidence of WSSV in the reproductive organs of black tiger shrimp Penaeus monodon. Since infected oocytes either undergo necrosis or fail to develop to maturity, transovarial transmission is an unlikely pathway. Transovum transmission is a very real possibility, however, and perhaps one of the most effective ways of dealing with this is simply to rinse or disinfect the nauplii.

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Detection of white spot baculovirus (WSBV) in giant freshwater prawn, Macrobrachium rosenbergii, using polymerase chain reaction

Cited by 80 publications

References 12 publications

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Experimental infection of white spot syndrome virus in freshwater crayfish Pacifastacus leniusculus

Virus-associated White Spot Syndrome of Shrimp in Taiwan: A Review.

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