Detection of VR-2332 Strain of Porcine Reproductive and Respiratory Syndrome Virus Type II Using an Aptamer-Based Sandwich-Type Assay

Lee, Su Jin; Kwon, Young Seop; Lee, Ji Eun; Choi, Eui Yul; Lee, Changhee; Song, Jae Young; Gu, Man Bock

doi:10.1021/ac3026866

Cited by 29 publications

(14 citation statements)

References 42 publications

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“… 3 - 5 To date, numerous high-affinity aptamers have been selected for a broad range of target molecules including metal ions, peptides, drugs, proteins, and even whole cells or viruses. 6 - 11 …”

Section: Introductionmentioning

confidence: 99%

Aptamers: Active Targeting Ligands for Cancer Diagnosis and Therapy

Wu¹,

Chen²,

Wu³

et al. 2015

Theranostics

218

137

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Aptamers, including DNA, RNA and peptide aptamers, are a group of promising recognition units that can specifically bind to target molecules and cells. Due to their excellent specificity and high affinity to targets, aptamers have attracted great attention in various fields in which selective recognition units are required. They have been used in biosensing, drug delivery, disease diagnosis and therapy (especially for cancer treatment). In this review, we summarized recent applications of DNA and RNA aptamers in cancer theranostics. The specific binding ability of aptamers to cancer-related markers and cancer cells ensured their high performance for early diagnosis of cancer. Meanwhile, the efficient targeting ability of aptamers to cancer cells and tissues provided a promising way to deliver imaging agents and drugs for cancer imaging and therapy. Furthermore, with the development of nanoscience and nanotechnology, the conjugation of aptamers with functional nanomaterials paved an exciting way for the fabrication of theranostic agents for different types of cancers, which might be a powerful tool for cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Aptamers: Active Targeting Ligands for Cancer Diagnosis and Therapy

Wu¹,

Chen²,

Wu³

et al. 2015

Theranostics

218

137

View full text Add to dashboard Cite

show abstract

“…Developped in the early 1990s [ 7 – 9 ], aptamers are three-dimensional oligonucleotides, either with a deoxy- (DNA) or a ribo-scaffold (RNA). They bind with high affinity and high specificity to a large variety of targets ranging from small organic molecules to viruses and live cells [ 10 – 12 ], by interaction optimized by shape complementarity. Aptamers are generated by an iterative process of in vitro selection and amplification called SELEX (Systematic Evolution of Ligands by EXponential enrichment) [ 13 ] and can be chemically-modified thus generating powerful tools for biomedical applications [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Ex Vivo and In Vivo Imaging and Biodistribution of Aptamers Targeting the Human Matrix MetalloProtease-9 in Melanomas

et al. 2016

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The human Matrix MetalloProtease-9 (hMMP-9) is overexpressed in tumors where it promotes the release of cancer cells thus contributing to tumor metastasis. We raised aptamers against hMMP-9, which constitutes a validated marker of malignant tumors, in order to design probes for imaging tumors in human beings. A chemically modified RNA aptamer (F3B), fully resistant to nucleases was previously described. This compound was subsequently used for the preparation of F3B-Cy5, F3B-S-acetylmercaptoacetyltriglycine (MAG) and F3B-DOTA. The binding properties of these derivatives were determined by surface plasmon resonance and electrophoretic mobility shift assay. Optical fluorescence imaging confirmed the binding to hMMP-9 in A375 melanoma bearing mice. Quantitative biodistribution studies were performed at 30 min, 1h and 2 h post injection of 99mTc-MAG-aptamer and 111In-DOTA-F3B. 99mTc radiolabeled aptamer specifically detected hMMP-9 in A375 melanoma tumors but accumulation in digestive tract was very high. Following i.v. injection of 111In-DOTA-F3B, high level of radioactivity was observed in kidneys and bladder but digestive tract uptake was very limited. Tumor uptake was significantly (student t test, p<0.05) higher for 111In-DOTA-F3B with 2.0%ID/g than for the 111In-DOTA-control oligonucleotide (0.7%ID/g) with tumor to muscle ratio of 4.0. Such difference in tumor accumulation has been confirmed by ex vivo scintigraphic images performed at 1h post injection and by autoradiography, which revealed the overexpression of hMMP-9 in sections of human melanomas. These results demonstrate that F3B aptamer is of interest for detecting hMMP-9 in melanoma tumor.

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“…Infectious diseases, such as the avian influenza virus, severe acute respiratory syndrome (SARS), hand-foot-and-mouth disease, influenza H1N1 and H7N9 are all threats to human health/life and collectively cause losses to social/economic development [2,3]. Therefore, developing methods for the rapid on-site assay of these diseases at an early stage is extremely important.…”

Section: Introductionmentioning

confidence: 99%

A Compact Immunoassay Platform Based on a Multicapillary Glass Plate

Xue

Zeng

Yang

et al. 2014

Sensors

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A highly sensitive, rapid immunoassay performed in the multi-channels of a micro-well array consisting of a multicapillary glass plate (MCP) and a polydimethylsiloxane (PDMS) slide is described. The micro-dimensions and large surface area of the MCP permitted the diffusion distance to be decreased and the reaction efficiency to be increased. To confirm the concept of the method, human immunoglobulin A (h-IgA) was measured using both the proposed immunoassay system and the traditional 96-well plate method. The proposed method resulted in a 1/5-fold decrease of immunoassay time, and a 1/56-fold cut in reagent consumption with a 0.05 ng/mL of limit of detection (LOD) for IgA. The method was also applied to saliva samples obtained from healthy volunteers. The results correlated well to those obtained by the 96-well plate method. The method has the potential for use in disease diagnostic or on-site immunoassays.

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Detection of VR-2332 Strain of Porcine Reproductive and Respiratory Syndrome Virus Type II Using an Aptamer-Based Sandwich-Type Assay

Cited by 29 publications

References 42 publications

Aptamers: Active Targeting Ligands for Cancer Diagnosis and Therapy

Aptamers: Active Targeting Ligands for Cancer Diagnosis and Therapy

Ex Vivo and In Vivo Imaging and Biodistribution of Aptamers Targeting the Human Matrix MetalloProtease-9 in Melanomas

A Compact Immunoassay Platform Based on a Multicapillary Glass Plate

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