Detection of viruses in avian faeces by direct electron microscopy

McNulty, M. S.; Curran, W. L.; Todd, D.; McFerran, J.B.

doi:10.1080/03079457908418349

Cited by 56 publications

(38 citation statements)

References 23 publications

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“…Faeces from control and infected birds were examined for viruses by direct electron microscopy using method C of McNulty et al (1979b). Faeces samples in which 1 to 5 rotavirus particles were observed per grid square were scored 1+ in Tables 1 and 2, those with 6 to 25 were scored 2+ and those with more than 25 were scored 3+.…”

Section: Methodsmentioning

confidence: 99%

Experimental infection of chickens with rotaviruses: Clinical and virological findings

McNulty

Allan²,

McCracken³

1983

Avian Pathology

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SUMMARYSpecific-pathogen-free chickens were infected orally with two different isolates (Ch 1 and 132) of chicken rotavirus. Chickens aged 1, 14 and 28 days were equally susceptible to infection. Following infection with either virus, the clinical signs were mild. Coincident with the peak of virus excretion and for a period of 1 to 2 days, infected birds passed increased quantities of caecal droppings. At necropsy, the caeca were abnormally distended with gas and fluid. Immunofluorescence showed that for both viruses the principal site of replication was the mature villous epithelial cell of the small intestine. However, Ch 1 rotavirus grew best in the duodenum while 132 virus favoured the jejunum and ileum. Ch 1 rotavirus and 132 virus were detected by direct electron microscopy in the faeces from 2 to 5 days and 1 to 7 days respectively after infection.

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Section: Methodsmentioning

confidence: 99%

Experimental infection of chickens with rotaviruses: Clinical and virological findings

McNulty

Allan²,

McCracken³

1983

Avian Pathology

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“…While, serological evidence of rotavirus infection in ducks has been recognised by McNulty et al (1979b), rotavirus isolation has not been reported from these species. As far as we know, this is the first report of the isolation of rotavirus from ducks.…”

Section: Text-fig 2 Neutralising Antibody Titres Of Field Ducks (60mentioning

confidence: 99%

“…turkeys, chickens and pheasants, by electron microscopy (McNulty et al, , 1979bJones et al, 1979;Gough et al, 1985). Also, serological evidence of rotavirus infection in chickens (McNulty et al, 1979b;Bartz et al, 1980;Sato et al, 1981;Pearson et al, 1982), turkeys and ducks (McNulty et al, 1979a) and pigeons (Vindevogel et al, 1981) has been reported. In addition, rotaviruses from chickens, turkeys and pheasants have been isolated in chicken kidney cell (CKC) cultures or chicken embryo liver cell (CEL) cultures (McNulty et ah, 1979a(McNulty et ah, , 1980(McNulty et ah, , 1981Yason and Schat, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…DISCUSSION In avian species, rotaviruses have been isolated from chickens, turkeys and pheasants (McNulty et al, 1979a(McNulty et al, , 1979b(McNulty et al, , 1980(McNulty et al, , 1981Yason and Schat, 1985). While, serological evidence of rotavirus infection in ducks has been recognised by McNulty et al (1979b), rotavirus isolation has not been reported from these species.…”

Section: Text-fig 2 Neutralising Antibody Titres Of Field Ducks (60mentioning

confidence: 99%

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Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures

et al. 1986

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“…Later, McNulty et al (1978) in Northern Ireland detected rotavirus in faeces from scouring turkey poults, and Jones et al (1979) demonstrated rotavirus in the faeces of chickens with diarrhoea in England. Since then, McNulty et al (1979b) have demonstrated rotavirus particles in the faeces and intestinal contents of turkeys with diarrhoea and in asymptomatic chickens, and have isolated and adapted turkey and chicken rotaviruses to serial replication in primary chick cell cultures (McNulty et aL, 1979a). Serological evidence of rotavirus infection has been demonstrated in chickens in the United States (Bartz et al, 1980) and in pigeons in Belgium (Vindevogel et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Two Avian Rotaviruses to Mammalian Cells and Characterization by Haemagglutination and RNA Electrophoresis

Hancock

William

Palmer

1983

Journal of General Virology

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SUMMARYTurkey and chicken rotaviruses were successfully adapted to replicate in a rhesus monkey embryo kidney (MA104) cell line. Trypsin treatment of virus and cells was essential for serial passage of the viruses. Polyacrylamide gel electrophoresis separated the viral RNA into 11 segments with clear resolution of segments 10 and I 1. Segment 5 migrated with size class I, and this characteristic appeared to be a unique feature of the avian rotavirus genome. The ability of the avian rotaviruses to haemagglutinate a variety of erythrocytes was demonstrated. A type-specific antigen was detected by haemagglutination-inhibition assays and a group or subgroup antigen by enzyme immunoassays. Treatment of serum with heparin-MnC12 was shown to be the best method for removing non-specific inhibitors of haemagglutination.

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Detection of viruses in avian faeces by direct electron microscopy

Cited by 56 publications

References 23 publications

Experimental infection of chickens with rotaviruses: Clinical and virological findings

Experimental infection of chickens with rotaviruses: Clinical and virological findings

Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures

Adaptation of Two Avian Rotaviruses to Mammalian Cells and Characterization by Haemagglutination and RNA Electrophoresis

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