Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system

Chang, Wen Hsin; Yang, Sung Yi; Lin, Chun-Han; Wang, Chih‐Hung; Li, Ping Chen; Chen, Tzong Yueh; Jan, Fuh Jyh; Lee, Gwo‐Bin

doi:10.1016/j.nano.2013.05.016

Cited by 31 publications

(21 citation statements)

References 36 publications

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“…To date, various liquids have been identified by different methods such as liquid chromatography [ [24] , [25] ] and microfluidics [ [26] , [27] , [28] , [29] ]. First, the molecules are separated and then detected by various methods such as ultraviolet, photoluminescence, fluorescence, electrochemical, refractive index and electrical conductivity.…”

Section: Ptsi/porous Si Schottky Junctionmentioning

confidence: 99%

Ultrasensitive bio-detection using single-electron effect

Ashoori

Naderpour

Ghezelayagh

et al. 2021

Talanta

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Nanosized particles can detect minute changes in surrounding electric fields via a phenomenon known as single-electron effect. Sensitivity can reach as much as counting single electrons. This phenomenon promises the possibilty of detection of atoms, molecules, cells or pathogens passing over or suspended around the nanosized particles whose IV characteristics is being monitored. Unfortunately, practical realization of such devices has been challenging because of their small size and low operating temperature, well below freezing temperature of regular liquids. Here we introduce PtSi/porous Si Schottky junction as a room temperature large size single-electron device capable of detecting very minute changes in the electric fields and hence identifying molecules, cells, and bacteria suspended or dissolved in liquid solutions. Encouraging results for detection of healthy and cancerous human cells as well as liquids and microorganisms are provided.

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Section: Ptsi/porous Si Schottky Junctionmentioning

confidence: 99%

Ultrasensitive bio-detection using single-electron effect

Ashoori

Naderpour

Ghezelayagh

et al. 2021

Talanta

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“…In LAMP-based systems, the amplification reaction precipitates out magnesium pyrophosphate, which makes the solution turbid [ 204 ]. Microfluidic chips can detect the changes by measuring light intensity reflected from the solution [ 205 ]. Lin et al [ 206 ] used optical fibers for light delivery and collection to reduce the variability of the signal from dust, dirt and manufacturing variability of the device.…”

Section: Detectionmentioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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“…The sensitivity referred to less than 24 CFU/mL for bacteria and 8.6 fg/µL for DNA [57]. Chuang et al demonstrated a simple, low-cost surface plasmon resonance sensing cartridge based on the LAMP method for the one-site detection of hepatitis B virus [58][59][60][61][62][63][64][65][66]. The HBV template could be detected by an SPR-LAMP system in 17 minutes even at the detection limit of 2 fg/mL.…”

Section: Strategies To Miniaturize Isothermal Amplification Reactionsmentioning

confidence: 99%

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

Tröger¹,

Niemann²,

Gärtig³

et al. 2015

J Nanomed Nanotechnol

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Nucleic acid amplification technologies (NAATs) offer the most sensitive tests in the clinical laboratory. These techniques are used as a powerful tool for screening and diagnosis of infectious diseases. Isothermal methods, as an alternative to polymerase chain reaction (PCR), require no thermocycling machine and can mostly be performed with reduced time, high throughput, and accurate and reliable results.However, current molecular diagnostic approaches generally need manual analysis by qualified and experienced personal which is a highly complex, time-consuming and labor-intensive task. Thus, the demand for simpler, miniaturized systems and assays for pathogen detection is steadily increasing. Microfluidic platforms and lab-on-a-chip devices have many advantages such as small sample volume, portability and rapid detection time and enable point-of-care diagnosis.In this article, we review several isothermal amplification methods and their implementation in microsystems in relation to quantification of nucleic acids. miniaturized systems can be diverse. The majority of publications are focussed on clinical diagnostics including foodborne organisms for environmental monitoring [10][11][12]. In case of an infectious disease, cultivation and phenotypic characterization are still the standard methods of microbiologists which need days up to weeks to obtain a diagnostic result. Hence, scientists started to make use of nucleic acid amplification methods to accelerate the analysis. The most widespread and well known technique for this purpose is the polymerase chain reaction (PCR) [10,13], which exploits the activity of the DNA polymerase. The method is based on a thermal cycling process consisting of repeated cycles of heating and cooling steps allowing for DNA melting, sequence specific primer binding and enzymatic amplification of the target nucleic acid. The quantitative assessment of target copies is possible by using a real-time PCR approach, where a concentration dependent signal (e.g. fluorescence) increases over time [14]. The latter enables the user to easily assess the pathogen load of patient samples [15][16][17][18]. Since the first example of a PCR on a miniaturized system in 1994 [19,20], numerous devices were presented, which integrate not just the amplification, but also sample preparation and detection steps [9,[21][22][23][24][25][26][27][28]. Just a few of those microsystems have reached the market so far, but some have shown a fascinating potential to replace the classical laboratory-oriented state-of-the art [29][30][31][32][33]. setups has been shown to obtain a similar result [34]. A smaller heat capacity allows for rapid changes in temperature beneficial for the PCR time as well as a higher parallelism of multiple genetic samples [34].However, a major drawback of the integration of PCR to microsystems is the necessity of sophisticated instrumentation. The reaction requires a thermal cycling instrumentation, space and considerable expertise [35]. Therefore, isothermal reactions for the ta...

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Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system

Cited by 31 publications

References 36 publications

Ultrasensitive bio-detection using single-electron effect

Ultrasensitive bio-detection using single-electron effect

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Isothermal Amplification and Quantification of Nucleic Acids and its Use in Microsystems

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