Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi

Yang, Feng; Li, Xiao Shan; Liang, Xian Wei; Zhang, Xiu Fang; Qin, Guang Sheng; Yang, Bing Zhuang

doi:10.1007/s11250-012-0143-z

Cited by 15 publications

(18 citation statements)

References 46 publications

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“…A total of 103 S. aureus isolates from 1021 clinical mastitis samples collected from 2013 to 2016 on 19 dairy farms in 9 provinces of China were used in this study (Table 1 ). Isolates were identified as S. aureus according to conventional methods, including Gram staining, colony morphology, hemolysis, catalase and tube coagulase test, as well as 16S rRNA, coa and nuc gene sequence analysis, as described [ 6 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

“…Allen et al [ 5 ] proposed that a combination of anti-virulence compounds targeting various virulence factors would be a more effective solution than conventional treatments. Therefore, characterization of virulence gene profiles and clonal diversity among S. aureus populations are very important in development of anti-virulence therapies [ 6 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

et al. 2018

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BackgroundStaphylococcus aureus, a common cause of bovine mastitis, is known for its ability to acquire to antimicrobial resistance and to secrete numerous virulence factors that can exacerbate inflammation. In addition, alpha-hemolysin has an important role in S. aureus infections, diversity of the hla gene (that produces alpha-hmolysin) in S. aureus isolated from bovine mastitis has not been well characterized. The objective was, therefore, to determine diversity of virulence genes, hla gene sequences, and clonal profiles of S. aureus from bovine mastitis in Chinese dairy herds, and to evaluate inter-relationships.ResultsThe antimicrobials resistance varies from as low as 1.9% (2/103) for CTX to as high as 76.7% (79/103) for penicilin in the 103 isolates and 46 (44.7%) S. aureus were determined as multi-resistant isolates with diverse resistance patterns. Thirty-eight virulence gene patterns (with variable frequencies) were identified in the 103 isolates and correlated with MLST types, indicating a great diversity. Although the hla gene also had great diversity (14 genotypes), Hla peptides were relatively more conserved. With 7 clonal complexes identified from 24 spa types and 7 MLST types. Regarding the letter, ST 97 was the dominant type in S. aureus from bovine mastitis in China. Furthermore, based on phylogenetic analysis, there was a distinct evolutionary relationship between the hla gene and MLST.ConclusionMulti-resistant S. aureus occurred in bovine mastitis with diverse resistance patterns. The diversity of virulence gene profiles, especially the hla gene and, their relationship with molecular types were reported for the first time in S. aureus from bovine mastitis, which will be useful for future studies on immunogenicity and vaccine development. In addition, based on the distinct evolutionary relationship between the hla gene and MLST types, we inferred that the hla gene has potential role for molecular typing of S. aureus.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1374-7) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

et al. 2018

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“…In this study, phenotypic identification revealed the incidence of 61% Staphylococcus aureus isolates 61% (61/100) was isolated from subclinical mastitis; this result was nearly consistent with Gianneechini et al, (2002) and Marija, et al, (2014) with ratio 62.8% and 56%, respectively. On the other hand, this prevalence rate is higher than that recorded in Egypt (Islam et al, 2014;Abdel-Kareem, 2015;Elhaig and Selim, 2015) (11.64%, 17.3%, 38.3 %, respectively); 8.0% in Colombia by Ramírez et al, (2014); 16.6% in Ugandan by Kasozi et al, (2014); 16.9% in China by Yang et al, (2015). Meanwhile this prevalence rate was somewhat lower than that recorded in Egypt by Abd El Tawab et al, (2015) (69%) and recorded in Brazil by Santos et al, (2014) (71.2%).…”

Section: Discussionmentioning

confidence: 65%

Prevalence and antimicrobial susceptibility pattern of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL-Sharkia Governorate

Tawab

Hofy

Ammar

et al. 2016

Benha Veterinary Medical Journal

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Subclinical mastitis is a disease of major economic importance to dairy industry causing reduced milk quality and loss in its production. Therefore, the present study was carried out for isolation and identification the main pathogen responsible for this disease and detection the drug of choice. A total of 475 milk samples of lactating dairy cows were collected from different localities in El-Sharkia Governorate, and subjected to physical, chemical tests (California mastitis test (CMT) as well as Somatic cell count (SCC), bacteriological examination and antimicrobial susceptibility pattern. The prevalence of subclinical mastitis at cow level was 21% (100/475). Accurately 80 Staphylococcal isolates were identified with prevalence of 61% S. aureus through beta hemolysis and coagulase positive. The obtained S. aureus isolates were highly sensitive to vancomycin, gentamicin, ciprofloxacin and chloramphenicol with percentages of 93.4%, 83.6%, 82% and 80.3%, respectively. Meanwhile, high frequency of resistance was observed to oxacillin, erythromycin and tetracycline, with percentages of 59%, 55.7%, and 47.5%, respectively. Therefore, the main choice drugs for subclinical mastitis were vancomycin and gentamicin.

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“…Também são reportadas altas frequências em pesquisas internacionais. Na Bélgica, as frequências foram de 98,7% (hla) e 99,1% (hlb) (Ote et al 2011); na Indonésia, as frequências foram de 84% (hla) e 16% (hlb) (Salasia et al 2011) e na China foram encontradas frequências de 85% para a hla e 82% para a hlb (Yang et al 2012). Observou-se que a toxina Hla do S. aureus provoca a morte de um número significativo de eosinófilos in vitro (Prince et al 2012).…”

Section: Discussionunclassified

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

et al. 2017

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RESUMO.-A capacidade de produção de toxinas pelo Staphylococcus aureus no leite e produtos derivados está relacionado com surtos de intoxicação alimentar. Objetivou-se nesta pesquisa, estudar a ocorrência de genes que codificam para enterotoxinas estafilocócicas (sea, seb, sed, seg, seh e sei) e toxinas α e β hemolítica (hla e hlb) em S. aureus isolados de 53 amostras de leite de tanques expansão comunitários no Estado de Alagoas, Brasil. Foram identificados 27 isolados (50,94%) como S. aureus pela amplificação do gene nuc. 13/27 isolados (48,1%) foram positivos para pelo menos um gene das enterotoxinas estudadas, sendo as frequências dos genes sea 33,3%, seh 18,5%, sei 11,1% e sed 7,4%; não entanto não foram identificados os genes seb e seg nestas bactérias. Para as toxinas hemolíticas, 51,9% dos isolados portavam ambos genes (hla e hlb), sendo a frequência para o gene hla de 81,5% e para o gene hlb de 51,9%. A frequência de genes das toxinas avaliadas é alta o que constitui um risco potencial para a saúde pública em especial, as enterotoxinas por serem termoestáveis e estarem asssociados com surtos de intoxicação alimentar. The capacity of toxin production by Staphylococcus aureus in milk and dairy products is associated with food poisoning outbreaks. The objective of this research was to study the frequency of genes encoding staphylococcal enterotoxin (sea, seb, sed, seg, seh and sei) and α and β hemolytic toxins (hla and hlb) in S. aureus isolates from 53 milk samples from community tanks in the State of Alagoas, Brazil. Twenty-seven isolates (50.94%) were identified as S. aureus by nuc gene amplification; 13/27 isolates (48.1%) were positive for at least one gene of the studied enterotoxins and the frequency of genes sea was 33.3%, seh 18.5%, sei 11.1% and sed 7.4%; the seb and sec genes have not been identified in the bacteria. For the hemolytic toxins, 51.9% of isolates harbored both genes (hla and hlb), the frequency of hla gene was 81.5% and 51.9% for the hlb gene. The evaluated toxin-encoding gene frequency is high and constitutes a potential risk for public health, especially staphylococcal enterotoxin genes; because they are heat-stable enterotoxins and have been associated with food poisoning.

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Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi

Cited by 15 publications

References 46 publications

Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

Prevalence and antimicrobial susceptibility pattern of Staphylococcus aureus isolated from dairy cattle's subclinical mastitis in EL-Sharkia Governorate

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

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