Detection of virion-associated MSRV-RNA in serum of patients with multiple sclerosis

Garson, Jeremy A.; Tuke, Philip W.; Giraud, P; Paranhos-Baccalà, Gláucia; Perron, Hervé

doi:10.1016/s0140-6736(98)24001-3

Cited by 154 publications

(116 citation statements)

References 2 publications

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“…Similar purifications have been employed prior to the detection of RT activity using the PERT assay (11,99,189,328). Nuclease treatment can also be used to degrade extraneous cellular DNA and RNA prior to amplification (164,440), because in theory, encapsidated RNA genomes should be protected.…”

Section: Vol 72 2008 Candidate Novel Retroviruses In Human Disease 167mentioning

confidence: 99%

“…Subsequently, antibodies that are reactive with HIV and HTLV antigens were identified in MS patients but were found only rarely in people with other neurological diseases or healthy controls (259,369,386,406). Although those serological data appeared to provide evidence to support the presence of a retrovirus in MS patients, it is now recognized that such antibody-based techniques can be unreliable in autoimmune diseases such as MS (164,392), and indeed, a number of groups were unable to confirm these data (199,247,291,366,520).…”

Section: Retroviruses Associated With Multiple Sclerosismentioning

confidence: 99%

See 1 more Smart Citation

Human RNA “Rumor” Viruses: the Search for Novel Human Retroviruses in Chronic Disease

Voisset

Weiss

Griffiths

2008

Microbiol Mol Biol Rev

143

156

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SUMMARY Retroviruses are an important group of pathogens that cause a variety of diseases in humans and animals. Four human retroviruses are currently known, including human immunodeficiency virus type 1, which causes AIDS, and human T-lymphotropic virus type 1, which causes cancer and inflammatory disease. For many years, there have been sporadic reports of additional human retroviral infections, particularly in cancer and other chronic diseases. Unfortunately, many of these putative viruses remain unproven and controversial, and some retrovirologists have dismissed them as merely “human rumor viruses.” Work in this field was last reviewed in depth in 1984, and since then, the molecular techniques available for identifying and characterizing retroviruses have improved enormously in sensitivity. The advent of PCR in particular has dramatically enhanced our ability to detect novel viral sequences in human tissues. However, DNA amplification techniques have also increased the potential for false-positive detection due to contamination. In addition, the presence of many families of human endogenous retroviruses (HERVs) within our DNA can obstruct attempts to identify and validate novel human retroviruses. Here, we aim to bring together the data on “novel” retroviral infections in humans by critically examining the evidence for those putative viruses that have been linked with disease and the likelihood that they represent genuine human infections. We provide a background to the field and a discussion of potential confounding factors along with some technical guidelines. In addition, some of the difficulties associated with obtaining formal proof of causation for common or ubiquitous agents such as HERVs are discussed.

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Section: Vol 72 2008 Candidate Novel Retroviruses In Human Disease 167mentioning

confidence: 99%

Section: Retroviruses Associated With Multiple Sclerosismentioning

confidence: 99%

Human RNA “Rumor” Viruses: the Search for Novel Human Retroviruses in Chronic Disease

Voisset

Weiss

Griffiths

2008

Microbiol Mol Biol Rev

143

156

View full text Add to dashboard Cite

show abstract

“…Although the etiology for slow progression in MS remains to be established, it has been speculated on a possible role e.g. for human endogenous retroviruses such as MSRV (Garson et al, 1998;Perron and Lang, 2010;Antony et al, 2010). Our knowledge about the cerebral actions of T-cell superantigens, e.g.…”

Section: Wwwintechopencommentioning

confidence: 99%

Superantigen-Mediated Encephalitis

Emmer¹,

Gerlach²,

Staege³

et al. 2011

Pathogenesis of Encephalitis

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“…MSRV is mainly known as virionassociated RNA identified by RT-PCR. [11][12][13][14] No specific MSRV locus has been identified in the human genome. The ambiguous use of various classifications of the involved proteins or RNAs as "MSRV-type" HERV-W, as "MSRV/HERV-W", or as "HERV-W pathogenic subtype MSRV" may explain why a specific locus for MSRV has yet to be identified.…”

mentioning

confidence: 99%