Detection of vaccinia virus DNA by quartz crystal microbalance

Kleo, Karsten; Kapp, Andreas; Ascher, L. Michael; Lisdat, Fred

doi:10.1016/j.ab.2011.07.016

Cited by 39 publications

(32 citation statements)

References 34 publications

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“…Hereby, some of the most promising results of DNA-QCM biosensors for different viruses are discussed. For instance, DNA-QCM biosensors have been successfully tested for the detection of hepatitis B virus (HepBV) [105][106][107], hepatitis C virus (HepCV) [117], vaccinia virus [108], viral haemorrhagic septicaemia virus (VHSV) [109], human papilloma virus (HPV) [110], dengue virus [118], and orchid viruses such as cymbidium mosaic virus (CymMV) and odontoglossum ringspot virus (ORSV) [111].…”

Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

“…These range from ions [122,123] or small molecules [124] to a wide range of proteins [125] or even entire cells [126,127]. As a result, aptamers Kleo et al [108] fabricated a DNA-QCM biosensor for indirect determination of vaccinia virus DNA using polymerase chain reaction (PCR) amplification. The biotinylated-capture-DNA was immobilized via affinity binding to NeutrAvidin, while NeutrAvidin was anchored to the QCM electrode by thiol moieties.…”

Section: Aptamersmentioning

confidence: 99%

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Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…However, these are still expensive due to the need for specialist equipment and staff [93][94]. PCR based methods also do not always correctly amplify viral nucleic acids when the viral concentration in samples is at a critically low level [95].…”

Section: Virus Detection and Viral Biosensing Systemsmentioning

confidence: 99%

Biosensors for waterborne viruses: Detection and removal

et al. 2015

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“…If the analytical signal is too weak to detect target materials, the detection sensitivity can be increased by introducing the signalenhancing step (Kurosawa et al, 2006). QCMI shows high detection sensitivity for biological materials even viruses (Bachelder et al, 2005;Eun et al, 2002;Kleo et al, 2011;Lee and Chang, 2005;Owen et al, 2007;Su et al, 2003;Susmel et al, 2000;Uttenthaler et al, 2001). Because a detection instrument for QCM is potable and QCM coated with virus-specific antibodies to detect plant viruses has long life span, it can be used for on site detection of plant viruses (Becker and Cooper, 2011;Eun et al, 2002).…”

Section: Quartz Crystal Microbalance Immunosensors (Qcmi)mentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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Detection of vaccinia virus DNA by quartz crystal microbalance

Cited by 39 publications

References 34 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Biosensors for waterborne viruses: Detection and removal

A Review of Detection Methods for the Plant Viruses

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