Detection of UV-induced cyclobutane pyrimidine dimers by near-infrared spectroscopy and aquaphotomics

Abstract

Ultraviolet (UV) radiation causes cellular DNA damage, among which cyclobutane pyrimidine dimers (CPDs) are responsible for a variety of genetic mutations. Although several approaches have been developed for detection of CPDs, conventional methods require time-consuming steps. Aquaphotomics, a new approach based on near-infrared spectroscopy (NIRS) and multivariate analysis that determines interactions between water and other components of the solution, has become an effective method for qualitative and quantitative parameters measurement in the solutions. NIR spectral patterns of UVC-irradiated and nonirradiated DNA solutions were evaluated using aquaphotomics for detection of UV-induced CPDs. Groups of UV-irradiated and nonirradiated DNA samples were classified (87.5% accuracy) by soft independent modeling of class analogy (SIMCA). A precise regression model calculated from NIR water spectral patterns based on UVC doses (r Val = 0.9457) and the concentration of cis-syn cyclobutane thymine dimers (cis-syn T<>Ts; r Val = 0.9993) was developed using partial least squares regression (PLSR), while taking advantage of water spectral patterns, particularly around 1400–1500 nm. Our results suggested that, in contrast to DNA, the formation of cis-syn T<>Ts increased the strongly hydrogen bonded water. Additionally, NIRS could qualitatively and quantitatively detect cis-syn T<>Ts in isolated DNA aqueous solutions upon UVC exposure.

Inflammation Due to Voriconazole‐induced Photosensitivity Enhanced Skin Phototumorigenesis in Xpa‐knockout Mice

“…Furthermore, CXCL1, a neutrophil attractant chemokine, is significantly upregulated in the blood serum of Xpa-knockout mice after UVB exposure and once the neutralizing antibody against CXCL1 was administered at every exposure of UVB, the number of developed skin tumors in Xpa-knockout mice actually decreased (8). Long-term use of the diuretic agent hydrochlorothiazide (HCTZ), another photosensitizing drug, was associated with an increased risk of nonmelanoma skin cancer (10,11) and we previously showed that HCTZ enhanced the formation of CPD after UVA irradiation (12). Taken together, the abnormal photosensitized skin inflammation caused by voriconazole or HCTZ may play a role in the development of nonmelanoma skin cancers.…”

Water Properties of Soft Contact Lenses: A Comparative Near-Infrared Study of Two Hydrogel Materials

“…Tsenkova has defined 12 characteristic water wavelength ranges which cover various conformations of water molecules and are very useful to present water spectral pattern [18]. The ranges of WAMACs were defined as follows: C1 (1336- [18,26]. Figure 6 shows the aquagrams of the hydrogel contact lenses representing the average of the pretreated spectra of the individual lenses across all replicas and experiments, at selected 12 characteristic water wavelengths from the WAMACs regions.…”

“…These water bands, called WAMACs [18], originate from the different water molecular conformations such as free water molecular species, water dimers, trimers, tetramers, or pentamers. Aquaphotomics approach was so far applied for various detection, qualitative descriptions, quantification, dynamic monitoring, and diagnostic purposes [18,[20][21][22][23][24][25][26].…”

Effects of photodynamic therapy on dermal fibroblasts from xeroderma pigmentosum and Gorlin-Goltz syndrome patients

“…In addition, formation of CDPs is cause of UV-induced cytotoxicity and mutagenicity, playing an important role in photocarcinogenesis [ 41 ]. In this work, CPDs evaluation confirmed that the low doses of UVA/UVB used to stimulate fibroblasts did not induce detectable damage.…”