Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea

Le, Van Phan; Kim, Jiyeon; Cho, Sung-Lim; Nam, Sun-Woo; Lim, Inseok; Lee, Hee-Joong; Kim, Ki-Jeong; Chung, Sunyang; Song, Wonkeun; Lee, Kyu Man; Rhee, Moon-Soo; Lee, Jung-Sook; Kim, Wonyong

doi:10.1002/jmv.21044

Cited by 50 publications

(61 citation statements)

References 79 publications

(119 reference statements)

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“…Rotavirus infection can cause afebrile convulsion 18,19,24,25) , but no such case was observed in our study. Nosocomial infection contributed to less than 1% of all rotavirus infected children 26) .…”

Section: ㆍ대한소아소화기영양학회지：제 14 권 제 2 호 2011contrasting

confidence: 63%

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Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis

Kang

et al. 2011

Korean J Pediatr Gastroenterol Nutr

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Purpose: We aimed to study the distribution of rotavirus genotypes (VP7 and VP4) and disease severity of rotavirus gastroenteritis prevalent in our community. Methods: Stool samples were collected from 156 children who were hospitalized with rotavirus gastroenteritis from December 2007 to June 2008. The disease severity of all patients was scored using the Vesikari scale. After extraction of ds-RNA of the rotavirus, cDNA synthesis using reverse transcription and polymerase chain reaction (RT-PCR) and multiplex PCR was performed. Following this, the final identification of genotypes was performed. Results: Of the 156 samples, VP7(G) and VP4(P) genotypes were identified in 147 (94.2%) and 140 (89.7%) samples, respectively. G1 (116 of 147 samples; 78.9%) and P[8] (137 of 140 samples; 97.9%) were the most prevalent, respectively. Of the 138 samples identified of combination types of VP7 and VP4, G1P[8] (111 samples; 80.4%) was the most prevalent. Other combination types varied with very low distribution rates. 9.4% of genotypes were not included in the new vaccines. The disease severity score was 11.8±3.3 (mean±2SD). The distribution of disease severity was mild or moderate in 37.8% and severe in 62.2% of patients. Conclusion:The most prevalent genotype combination of rotavirus was G1P[8] and genotypes not included in the vaccines represented 9.4% in our community. Disease severity distribution of hospitalized children with rotavirus gastroenteritis was higher in the severe than in the mild and moderate categories.

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Section: ㆍ대한소아소화기영양학회지：제 14 권 제 2 호 2011contrasting

confidence: 63%

“…19) report in Seoul, G1P [8] was the most common genotype (80.4%) in our study (Table 2). On the other hand, Han et al 20) reported the most frequent combination of genotypes was G9P [8] (32.1%) followed by G1P [8] (20.7%) in Seoul.…”

Section: ㆍ대한소아소화기영양학회지：제 14 권 제 2 호 2011supporting

confidence: 59%

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis

Kang

et al. 2011

Korean J Pediatr Gastroenterol Nutr

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“…According to the literature, although P[4] and P[6] are predominant in countries such as Brazil for P[4] (Soares et al, 2012) and Nigeria for P[6] (Aminu et al, 2010), P[8] is known to represent the most frequent VP4 genotype worldwide (Cunliffe et al, 2009;Esona et al, 2010;Le et al, 2008;Uchida et al, 2006).…”

Section: G/p-typingmentioning

confidence: 99%

Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Moussa

Fredj

Fodha

et al. 2016

Journal of Medical Microbiology

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Group A rotavirus (RVA) represents the most important aetiological agent of diarrhoea in children worldwide. From January 2009 to December 2014, a multi-centre study realized through 11 Tunisian cities was undertaken among children aged <5 years consulting or hospitalized for acute gastroenteritis. A total of 1127 faecal samples were collected. All samples were screened by ELISA for the presence of RVA antigen. RVA-positive samples were further analyzed by PAGE and used for G/P-genotyping by semi-nested multiplex RT-PCR. Globally, 270 specimens (24 %) were RVA-positive, with peaks observed annually between November and March. Nine different electropherotypes could be visualized by PAGE, six with a long profile (173 cases) and two with a short one (seven cases). Mixed profiles were detected in two cases. Among the 267 VP7 genotyped strains, the predominant G-genotype was G1 (39.6 %) followed by G3 (22.2 %), G4 (13 %), G9 (11.5 %), G2 (5.2 %) and G12 (5.2 %). Among the 260 VP4 genotyped strains, P[8] genotype was the predominant (74. 9 %). Uncommon G/Pgenotype combinations, mixed infections and untypeable strains were also detected. This is the first report, in Tunisia, of multiple detection of an emerging human RVA strain, G12 genotype. This study highlighted the need for maintaining active surveillance of emerging strains in Northern Africa.

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“…Non-typeable samples might be the result of a false-positive EIA, the presence of novel strains, the nonuse of specific primers for rare genotypes such as G5, G6, G11 or the failure in RT-PCR technique (WHO, 2009). Because of the appearance of unusual rotavirus strains such as G10, G12 from South Korea (Le et al, 2008), Italy (Zuccotti et al, 2010), France (De Rougemont et al, 2011) and other countries (Pun et al, 2007;Annarita et al, 2010), in this research were used from the specific primers them for the first time in Iran, but none of them were observed in this study. Overall, 79.54% of hospitalizations due to rotavirus gastroenteritis occurred in children younger than 2 years more than in the older age groups, as was found in previous investigations in various countries (Khalili et al, 2004;Pun et al, 2007;Lacroix et al, 2010;Mladenova et al, 2010).…”

Section: Discussionmentioning

confidence: 93%

“…Studies of the genotyping in different regions of the world have been indicated that G1-G4 and G9 genotypes are the most common genotypes detected in children diarrheal disease (Jiraphongsa et al, 2005;Annarita et al, 2010;Tatte et al, 2010). But in recent years, other rare or uncommon rotavirus genotypes, such as G5, G8, G10, G11 and G12, have been reported in many countries (Rahman et al, 2005;Le et al, 2008;Mast et al, 2010;Zuccotti et al, 2010). The directed rotavirus surveillance in all countries is significant to determine precisely the prevalence of rotavirus gastroenteritis, circulating genotypes and assessing the need for general vaccination of children and infants, particularly in the developing regions of the world.…”

Section: Introductionmentioning

confidence: 99%

Genotypic distribution of rotavirus strains causing severe gastroenteritis in children under 5 years old in Borazjan, Iran

Kargar¹,

Najafi²,

Zandi³

et al. 2011

Afr. J. Microbiol. Res.

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Rotaviruses are recognized as the most common causes of severe gastroenteritis and death among children worldwide. The aim of this study was to evaluate the disease burden of rotavirus gastroenteritis and the prevalence of different G genotypes of rotaviruses circulating in children aged <5 years old who were hospitalized for acute gastroenteritis in Borazjan City, Iran. This cross sectional-descriptive study was done on 316 fecal samples collected from children aged <5 years old with acute gastroenteritis. All the stool specimens were tested for rotavirus with enzyme immunoassays (EIA). Rotavirus-positive specimens were genotyped by the Nested reverse transcription polymerase chain reaction (RT-PCR) and using different type specific primers. Out of total collected samples rotavirus infection was detected in 88 (27.85%). Of the rotavirus episodes, 79.54% occurred during the first 2 years of life, with the peak prevalence of severe rotavirus disease occurring in cold seasons. Among the common genotypes, G1 was the most predominant (52.27% of strains) and other identified genotypes included non-typeable, G9 and G4, 40.90%, 4.54% and 2.27% of isolates, respectively. Because of the high frequency of rotavirus infection it is important to continue rotavirus surveillance in the other regions of Iran to determine accurately the burden of rotavirus disease and the emerging new genotypes. This will assist policy makers in decision making on rotavirus vaccine introduction.

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Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea

Cited by 50 publications

References 79 publications

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis

Distribution of rotavirus VP7 and VP4 genotypes circulating in Tunisia from 2009 to 2014: Emergence of the genotype G12

Genotypic distribution of rotavirus strains causing severe gastroenteritis in children under 5 years old in Borazjan, Iran

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