Detection of unusual Cryptosporidium parvum subtype in patients with gastrointestinal cancer in Egypt

Ali, Mona Ibrahim; Wahab, Wegdan M. Abd El; Hassan, Ahmed; Ryan, Una; Xiao, Lihua; Arafa, Waleed M.; Hamdy, Doaa A.

doi:10.1007/s00436-022-07761-8

Cited by 3 publications

(2 citation statements)

References 69 publications

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“…The mechanisms by which these parasites in uence the gastrointestinal tract of their host is unclear. Gastrointestinal malignancies mortality, accounting for nearly one-third of all cancer incidences worldwide [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

The efficacy of modified Ziehl-Neelsen (MZN) and antigen detection enzyme linked immunosorbent assay (ELISA) for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients.

Abdullah,

Taha,

Taher

et al. 2024

Preprint

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Background: Cryptosporidiosis diagnosis based on demonstration of the parasite microscopically in the stool through modified Ziehl-Neelsen (MZN) or auramine phenol staining techniques. The primary goal of this study was to compare between the efficacy of cryptospridia antigen detection potential in the fecal specimens using ELISA and Oocyst detection by MZN staining methods for the diagnosis of human cryptosporidiosis among some Sudanese patients with gastrointestinal cancer. One hundred and eighty-one patients suspected of having gasterointestinal cancer based on their clinical presentation were enrolled in this study. Cryptosporidiosis was diagnosed using polymerase chain reaction (PCR). MZN staining was conducted for fresh stool samples to detect Cryptosporidium Oocyst. Results: Of 181 cancer suspected patients, 55 (30.5%) were confirmed as having gastrointestinal cancer. Of the 55 cancer patients 41(74.5%) were confirmed as having colorectal cancer, 7 (12.7%) small intestine cancer, 4(7.3%) liver cancer, 3(5.5%) esophageal cancer. Of the confirmed cancer patients MZN identified 24 (43.6%) and ELISA identified 31(56.4%) as having cryptospridia Oocyst and antigens in their fecal samples, respectively. Based on PCR outcome, the infection rates was significantly higher in colorectal cancer patients than in other cancer patients (P = 0.02). Depending on the criteria of ‘true positive’ samples, sensitivity of MZN and ELISA was 81.06% and 94.25% respectively. Conclusions: Fecal antigen ELISA detection test has the advantage of accurate diagnosis of Cryptosporidiosis over MZN staining technique especially in immunocompromised patients.

show abstract

Section: Introductionmentioning

confidence: 99%

The efficacy of modified Ziehl-Neelsen (MZN) and antigen detection enzyme linked immunosorbent assay (ELISA) for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients.

Abdullah,

Taha,

Taher

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Despite being well known as a self-limiting illness in immunocompetent people, cryptosporidiosis is regarded as a public health issue because it can cause a life-threatening disease in immunosuppressed patients and young children, with significant high mortality and morbidity rates (Ghomashlooyan et al, 2015). Furthermore, Cryptosporidium was recently found in gastrointestinal cancer patients, indicating a possible link (Ali et al, 2023;Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Hamdy,

Ismail,

El‐Askary

et al. 2024

Microscopy Res & Technique

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Cryptosporidiosis is a global health problem threats life of immunocompromised patients. Allium sativum (A. sativum) is one of the therapeutic options for cryptosporidiosis. This study develops green synthesized ZnO‐NPs based on A. sativum extract, and assesses its therapeutic application in treating experimental cryptosporidiosis in immunosuppressed mice. FTIR, scanning electron microscopy, and zeta analyzer were used for characterization of bio ZnO‐NPs. The morphology of prepared materials appeared as sponge with many pores on the whole surface that allows the feasibility of bio ZnO‐NPs for different biological activities. Its structural analysis was highly stabilized with negative charge surface which indicated for well distribution into the parasite matrix. Twenty‐five immunosuppressed Cryptosporidium parvum infected mice, classified into 5 groups were sacrificed at 21th day after infection with evaluation of parasitological, histopathological, oxidative, and proinflammatory biomarkers. Treated mice groups with 50 and 100 mg/kg of AS/ZnO‐NPs showed a highly significant decline (79.9% and 83.23%, respectively) in the total number of expelled oocysts. Both doses revealed actual amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. They also significantly produced an increase in GSH values and improved the changes in NO and MDA levels, and showed high anti‐inflammatory properties. This study is the first to report green synthesis of ZnO/A. sativum nano‐composite as an effective therapy in treating cryptosporidiosis which gave better results than using A. sativum alone. It provides an economical and environment‐friendly approach towards novel delivery synthesis for antiparasitic applications.Research Highlights Green synthesis of ZnO‐NPs was developed using A. sativum extract. The morphology of prepared ZnO‐NPs appeared as sponge with many pores on SEM The study evaluates its therapeutic efficacy against murine cryptosporidiosis The green synthesized ZnO‐NPs significantly reduced percent of oocyst shedding, improved the pathological changes, and showed high antioxidant and anti‐inflammatory potentials.

show abstract

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

Abdullah,

Taha,

Taher

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Cryptosporidiosis diagnosis based on demonstration of the parasite microscopically in the stool through modified Ziehl-Neelsen (MZN) or auramine phenol staining techniques. The primary goal of this study was to compare between the efficacy of cryptospridia antigen detection potential in the fecal specimens using ELISA and Oocyst detection by MZN staining methods and molecular techniques for the diagnosis of human cryptosporidiosis among some Sudanese patients with gastrointestinal cancer. One hundred and eighty-one patients suspected of having gasterointestinal cancer based on their clinical presentation were enrolled in this study. Cryptosporidiosis was diagnosed using polymerase chain reaction (PCR). MZN staining was conducted for fresh stool samples to detect Cryptosporidium Oocyst. Results: Of 181 cancer suspected patients, 55 (30.5%) were confirmed as having gastrointestinal cancer. Of the 55 cancer patients 41(74.5%) were confirmed as having colorectal cancer, 7 (12.7%) small intestine cancer, 4(7.3%) liver cancer, 3(5.5%) esophageal cancer. Of the confirmed cancer patients MZN identified 24 (43.6%) and ELISA identified 31(56.4%) as having cryptospridia Oocyst and antigens in their fecal samples, respectively. Based on PCR outcome, the infection rates was significantly higher in colorectal cancer patients than in other cancer patients (P = 0.02). Depending on the criteria of ‘true positive’ samples, sensitivity of MZN and ELISA was 81.06% and 94.25% respectively. Conclusions: Fecal antigen ELISA detection test has the advantage of accurate diagnosis of Cryptosporidiosis over MZN staining technique especially in immunocompromised patients.

show abstract

Detection of unusual Cryptosporidium parvum subtype in patients with gastrointestinal cancer in Egypt

Cited by 3 publications

References 69 publications

The efficacy of modified Ziehl-Neelsen (MZN) and antigen detection enzyme linked immunosorbent assay (ELISA) for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients.

The efficacy of modified Ziehl-Neelsen (MZN) and antigen detection enzyme linked immunosorbent assay (ELISA) for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients.

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

The efficacy of modified Ziehl-Neelsen (MZN) and enzyme linked immunosorbent assay (ELISA) and molecular techniques for detection of cryptosporidia in the stool of gastrointestinal cancer Sudanese patients

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