Detection of unprecedented level of antibiotic resistance and identification of antibiotic resistance factors, including QRDR mutations in<i>Escherichia coli</i>isolated from commercial chickens from North India

Rawat, Nitish; Anjali, .; Jamwal, Rohit; Devi, Pukhrambam Pushpa; Yadav, Karuna; Rajagopal, Raman

doi:10.1111/jam.15209

Cited by 8 publications

(7 citation statements)

References 41 publications

(65 reference statements)

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“…The ARGs in the integron can be arranged in different combinations [ 34 , 35 ]. It has been reported that the dfr ( dfr A16, dfr A17) + aad ( aad A1, aad A2, aad A5) gene family in the ARG boxes is the most common combination, encoding dihydrofolate reductase and aminoglyco adenosyltransferase, respectively [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in E. Coli of animal origin and discovery of a novel ICE mobile element in Northeast China

Zhu,

Ding,

et al. 2023

BMC Vet Res

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Background Multidrug resistance in Enterobacteriaceae including resistance to quinolones is rising worldwide. The development of resistance may lead to the emergence of new transmission mechanisms. In this study, the collection of different E. coli was performed from animals and subjected to subsequent procedures including pulsed-field gel electrophoresis, micro-broth dilution method, polymerase chain reaction. Whole genome sequencing of E. coli C3 was performed to detect the affinity, antimicrobial resistance and major carriers of the isolates. Results A total of 66 E. coli were isolated and their antibiotic resistance genes, frequency of horizontal transfer and genetic environment of E. coli C3 were determined. The results showed there were both different and same types in PFGE typing, indicating clonal transmission of E. coli among different animals. The detection of antimicrobial resistance and major antibiotic resistance genes and the plasmid transfer results showed that strains from different sources had high levels of resistance to commonly used clinical antibiotics and could be spread horizontally. Whole-genome sequencing discovered a novel ICE mobile element. Conclusion In summary, the antimicrobial resistance of E. coli in northeast China is a serious issue and there is a risk of antimicrobial resistance transmission. Meanwhile, a novel ICE mobile element appeared in the process of antimicrobial resistance formation.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in E. Coli of animal origin and discovery of a novel ICE mobile element in Northeast China

Zhu,

Ding,

et al. 2023

BMC Vet Res

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“…Its association with CRRs on ColV virulence plasmids, such as pCERC3 [ 37 ] in commensal E. coli from healthy humans [ 38 ], is concerning. sul3 , first described in E. coli from swine [ 39 ], has since been found to be globally distributed in swine [ 40 ], humans [ 34 ], poultry [ 41 , 42 ] and wild birds [ 43 ]. The gene forms part of a sul3 -encoding conserved segment ( sul3 -CS) that replaced the sul1 -containing 3′-CS that is typical of class 1 integrons [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis of an I1 Plasmid Hosting a sul3-Class 1 Integron and blaSHV-12 within an Unusual Escherichia coli ST297 from Urban Wildlife

2022

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Wild birds, particularly silver gulls (Chroicocephalus novaehollandiae) that nest near anthropogenic sites, often harbour bacteria resistant to multiple antibiotics, including those considered of clinical importance. Here, we describe the whole genome sequence of Escherichia coli isolate CE1867 from a silver gull chick sampled in 2012 that hosted an I1 pST25 plasmid with blaSHV-12, a β-lactamase gene that encodes the ability to hydrolyze oxyimino β-lactams, and other antibiotic resistance genes. Isolate CE1867 is an ST297 isolate, a phylogroup B1 lineage, and clustered with a large ST297 O130:H11 clade, which carry Shiga toxin genes. The I1 plasmid belongs to plasmid sequence type 25 and is notable for its carriage of an atypical sul3-class 1 integron with mefB∆260, a structure most frequently reported in Australia from swine. This integron is a typical example of a Tn21-derived element that captured sul3 in place of the standard sul1 structure. Interestingly, the mercury resistance (mer) module of Tn21 is missing and has been replaced with Tn2-blaTEM-1 and a blaSHV-12 encoding module flanked by direct copies of IS26. Comparisons to similar plasmids, however, demonstrate a closely related family of ARG-carrying plasmids that all host variants of the sul3-associated integron with conserved Tn21 insertion points and a variable presence of both mer and mefB truncations, but predominantly mefB∆260.

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“…In this study, all three isolates displayed mutations in the QRDR region of gyrA and parC , which has also been reported in many investigations. 30 …”

Section: Discussionmentioning

confidence: 99%

“…In this study, all three isolates displayed mutations in the QRDR region of gyrA and parC, which has also been reported in many investigations. 30 The IncFII plasmids are highly mobile and carry several bla NDM variants in Enterobacteriaceae. 44 A recent study has shown the ability of bla NDM bearing IncFII plasmid transfer to different members of Enterobacteriaceae along with the other ARGs.…”

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confidence: 99%

Characterization of NDM-5 Carbapenemase-Encoding Gene (blaNDM-5) – Positive Multidrug Resistant Commensal Escherichia coli from Diarrheal Patients

Chowdhury¹,

Ramamurthy²,

Das³

et al. 2022

IDR

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Purpose The multidrug resistance Enterobacteriaceae cause many serious infections resulting in prolonged hospitalization, increased treatment charges and mortality rate. In this study, we characterized bla NDM-5 -positive multidrug resistance commensal Escherichia coli (CE) isolated from diarrheal patients in Kolkata, India. Methods Three CE strains were isolated from diarrheal stools, which were negative for different pathogroups of diarrheagenic E. coli (DEC). The presence of carbapenemases encoding genes and other antimicrobial resistance genes (ARGs) was detected using PCR. The genetic arrangement adjoining bla NDM-5 was investigated by plasmid genome sequencing. The genetic relatedness of the strains was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) methods. Results In addition to colistin, the bla NDM-5 -positive CE strains showed resistance to most of the antibiotics. Higher MICs were detected for ciprofloxacin (>32 mg/L) and imipenem (8 mg/L). Molecular typing revealed that three CE strains belonged to two different STs (ST 101 and ST 648) but they were 95% similar in the PFGE analysis. Screening for ARGs revealed that CE strains harbored Int-1, bla TEM , bla C TX-M3 , bla OXA-1 , bla OXA-7 , bla OXA-9 , tetA, strA, aadA1, aadB, sul2, floR, mph(A) , and aac(6´)-Ib-cr . In conjugation experiment, transfer frequencies ranged from 2.5×10 −3 to 8.4x10 −5 . The bla NDM-5 gene was located on a 94-kb pNDM-TC-CE-89 type plasmid, which is highly similar to the IncFII plasmid harboring an IS26-IS30- bla NDM-5 - ble MBL - trpF-dsbd -IS 91-dhps structure. Conclusion To the best of our knowledge, this is the first report on carbapenem resistance involving the bla NDM-5 gene in CE from diarrheal patients. The circulation of bla NDM-5 gene in CE is worrisome, since it has the potential to transfer bla NDM-5 gene to other enteric pathogens.

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Detection of unprecedented level of antibiotic resistance and identification of antibiotic resistance factors, including QRDR mutations inEscherichia coliisolated from commercial chickens from North India

Cited by 8 publications

References 41 publications

Antimicrobial resistance in E. Coli of animal origin and discovery of a novel ICE mobile element in Northeast China

Antimicrobial resistance in E. Coli of animal origin and discovery of a novel ICE mobile element in Northeast China

Genomic Analysis of an I1 Plasmid Hosting a sul3-Class 1 Integron and blaSHV-12 within an Unusual Escherichia coli ST297 from Urban Wildlife

Characterization of NDM-5 Carbapenemase-Encoding Gene (blaNDM-5) – Positive Multidrug Resistant Commensal Escherichia coli from Diarrheal Patients

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