Detection of unlabeled particles in the low micrometer size range using light scattering and hydrodynamic 3D focusing in a microfluidic system

Zhuang, Guisheng; Jensen, Thomas Glasdam; Kutter, Jörg Peter

doi:10.1002/elps.201100674

Cited by 13 publications

(12 citation statements)

References 40 publications

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“…High performance multi-angle scattering approaches have been developed using cytometers with free space optics [15][16][17][18] but have not been demonstrated in an integrated cytometer. Such optofluidic technology which combines microfluidics and integrated optical waveguides [19][20][21][22] offers an ideal platform with which to develop cost effective and high performance flow cytometers dedicated for EV analysis using multi-angle scattering. For example, as will be demonstrated in this paper, an angular array of collection waveguides 23 can be used to measure the light scattered at multiple discrete angles providing a relative scattering distribution for model fitting to calculate diameter.…”

Section: Introductionmentioning

confidence: 99%

Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering

et al. 2020

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“…1. The reconfigurability of fluids in optofluidic systems promotes broad applications such as reconfigurable lenses 14 , 3D dye lasers 15 . Moreover, the diffusion between miscible liquids or heat conduction in liquids can introduce the natural gradient index (GRIN) medium (Inset (i)-(2)).…”

Section: Introductionmentioning

confidence: 99%

Optofluidics: the interaction between light and flowing liquids in integrated devices

Zhu¹,

Zhu²,

Zuo³

et al. 2019

Opto-Electronic Advances

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Optofluidics is a rising technology that combines microfluidics and optics. Its goal is to manipulate light and flowing liquids on the micro/nanoscale and exploiting their interaction in optofluidic chips. The fluid flow in the on-chip devices is reconfigurable, non-uniform and usually transports substances being analyzed, offering a new idea in the accurate manipulation of lights and biochemical samples. In this paper, we summarized the light modulation in heterogeneous media by unique fluid dynamic properties such as molecular diffusion, heat conduction, centrifugation effect, light-matter interaction and others. By understanding the novel phenomena due to the interaction of light and flowing liquids, quantities of tunable and reconfigurable optofluidic devices such as waveguides, lenses, and lasers are introduced. Those novel applications bring us firm conviction that optofluidics would provide better solutions to high-efficient and high-quality lab-on-chip systems in terms of biochemical analysis and environment monitoring.

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“…Also the 3D focusing can be achieved through centrifugal forces 6,7 exploiting simple and planar structures, but a crucial point in these cases is represented by the flow rates at which the devices are supposed to work, thus leading to less flexible devices. Finally, few examples of multi-level devices 8,9,10,11,12 proved to be quite efficient in the realization of 3D hydrodynamic focusing also tested as cytometers. Anyway these approaches generally leads to complicated fabrication processes and the devices require the assembly of external component for particle detection and counting.…”

Section: Introductionmentioning

confidence: 99%

Monolithic cell counter based on 3D hydrodynamic focusing in microfluidic channels

2014

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Hydrodynamic focusing is a powerful technique frequently used in microfluidics that presents a wide range of applications since it allows focusing the sample flowing in the device to a narrow region in the center of the microchannel. In fact thanks to the laminarity of the fluxes in microchannels it is possible to confine the sample solution with a low flow rate by using a sheath flow with a higher flow rate. This in turn allows the flowing of one sample element at a time in the detection region, thus enabling analysis on single particles. Femtosecond laser micromachining is ideally suited to fabricate device integrating full hydrodynamic focusing functionalities thanks to the intrinsic 3D nature of this technique, especially if compared to expensive and complicated lithographic multi-step fabrication processes. Furthermore, because of the possibility to fabricate optical waveguides with the same technology, it is possible to obtain compact optofluidic devices to perform optical analysis of the sample even at the single cell level, as is the case for optical cell stretchers and sorters. In this work we show the fabrication and the fluidic characterization of extremely compact devices having only two inlets for 2D (both in vertical and horizontal planes) as well as full 3D symmetric hydrodynamic focusing. In addition we prove one of the possible application of the hydrodynamic focusing module, by fabricating and validating (both with polystyrene beads and erythrocytes) a monolithic cell counter obtained by integrating optical waveguides in the 3D hydrodynamic focusing device.

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Detection of unlabeled particles in the low micrometer size range using light scattering and hydrodynamic 3D focusing in a microfluidic system

Cited by 13 publications

References 40 publications

Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering

Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering

Optofluidics: the interaction between light and flowing liquids in integrated devices

Monolithic cell counter based on 3D hydrodynamic focusing in microfluidic channels

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