Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study

Abstract

Background Separation of the epithelial lining from the underlying connective tissue wall has been a frequently observed and unique feature in odontogenic keratocysts (OKC), but not in other odontogenic cysts nor neoplasms. No study on OKC has been reported evaluating the role of type VII Collagen, the anchoring fibrils, which function in stabilising the epithelial structure. The purpose of this study was to assess the role of type VII collagen in the fragility of the epithelium leading to a high recurrence rate in OKCs. Material and Methods Immunohistochemical staining with Abcam® Monoclonal Mouse Anti-Collagen VII Antibody [LH7.2] (used at a dilution of 1:200) on 30 tissues of OKC. The chi-square test was applied to confirm the statistical significance between the control and test groups. The frequencies of the pattern of distribution for the staining characteristics of collagen VII were calculated in the OKC samples. Results Out of the 30 OKC samples 22 (73.3%) showed negative staining for type VII Collagen. Among the infected cases, 7 showed a positive basement membrane staining and one of the non-infected OKC showed positive basement membrane staining. However, none of the syndrome associated or recurrent OKCs showed any evidence of type VII collagen reactivity. Conclusions Considering the distribution of type VII collagen in OKCs it can be concluded that type VII collagen expression is altered in OKCs, leading to destabilisation of the epithelium connective tissue interface thus rendering the epithelium-connective tissue interface fragile. Key words: Type VII Collagen, Odontogenic Keratocyst, Basal Lamina, Immunohistochemistry.

In Silico Analysis of Genes Associated with the Pathogenesis of Odontogenic Keratocyst

“…In relation to collagen's expression pattern, our analysis showed the expression of fibrillar (COL5A2, COL11A1, COL12A1, COL5A1, COL1A1, COL1A2, COL3A1, and COL6A1) and basement membranes (COL4A2 and COL8A1)-related proteins. Cota et al analyzed the expression of collagen VII, a fibril restricted to the basement zone beneath stratified squamous epithelia; however, they found a discontinuous immunoexpression pattern that could be associated with the expression of other collagen subtypes and by the collagenolysis related to MMP2 and MMP13 [28]. It is possible that the potential of small satellite or daughter cysts development by the budding of the basal layer could be related to that affection of the basement zone; this is a histological feature and behavior that could favor OK recurrence.…”

Automated Detection of Collagen Bundles in Second Harmonic Generation Microscopy Images

“…As a result of that, objects can be detected in several disconnected components and due to that it may result with high number of false positive detection. Some other methods from the literature are out of focus of this paper since the tasks and/or data acquisition methods are not comparable [26], [27], [28], [29], [30], [31], [32], [33], [34], [35], [36].…”