Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays

Watson, Mark; Lopatin, Dennis E.; Bretz, Walter A.; Ertel, Inta J.; Loesche, Walter J.

doi:10.1177/00220345910700070701

Cited by 30 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e.g., a plaque sample can be established by the hydrolysis of a synthetic trypsin substrate, N-benzoyl-~~-arginine-2-naphthylReceiwd 24 Sept. 1992; accepted 18 Nov. 1992.…”

Section: Fiwsjrhus T Ifenticola and A Capnocj*tophagu Sp'o-'~mentioning

confidence: 99%

Prevalence of Treponema denticola and Porphyromonas gingivalis in plaque from periodontally-healthy and periodontally-diseased sites

Wilson¹,

Lopatin²,

Osborne³

et al. 1993

Journal of Medical Microbiology

View full text Add to dashboard Cite

Summary. Intracrevicular plaque from periodontally-healthy individuals who had refrained from oral hygiene measures for 24 h prior to sampling, and subgingival plaque from diseased sites of patients with chronic periodontitis were screened by ELISA for the presence of Porph~~omonns gingirdis and Treponema denticola. The samples were also subjected to the PerioScan test to detect the presence of enzymes capable of degrading N-benzoyl-DL-arginine-Z-naphthylamide (BANA). Of the 14 1 samples from periodontally-healthy sites, 73 O h contained T. denticola antigens and 78 * / o P. gingiualis antigens, compared to 43 YO and 59 YO, respectively, in plaque samples from the 159 diseased sites. A positive reaction in the PerioScan test was obtained in 89% of plaque samples from diseased sites and in 60% of those from healthy sites. The correlation between the results of the two assays was poor in the case of intracrevicular plaque from healthy sites. However, with plaque samples from diseased sites, the results of the PerioScan test showed very strong correlation with those obtained with the ELISA, suggesting that the former may be a useful, rapid means of indicating the presence of T. denticola and P. gingicalis in such plaque samples.

show abstract

“…e.g., a plaque sample can be established by the hydrolysis of a synthetic trypsin substrate, N-benzoyl-~~-arginine-2-naphthylReceiwd 24 Sept. 1992; accepted 18 Nov. 1992.…”

Section: Fiwsjrhus T Ifenticola and A Capnocj*tophagu Sp'o-'~mentioning

confidence: 99%

Prevalence of Treponema denticola and Porphyromonas gingivalis in plaque from periodontally-healthy and periodontally-diseased sites

Wilson¹,

Lopatin²,

Osborne³

et al. 1993

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…There are few data concerning the prevalence of P. gingivalis, A. actinomycetemcomitans, and T. forsythensis in the plaque of healthy children. The available prevalence data for these pathogens are also contradictory; for example, P. gingivalis has been isolated from the plaque of 80% of children during and after puberty (28), whereas other studies failed to detect P. gingivalis in prepubertal children (9,31). Although periodontal disease is rare in healthy children, it is important to investigate the presence of periodontal pathogens as the permanent teeth start to erupt.…”

mentioning

confidence: 99%

Prevalence of Periodontal Pathogens in Dental Plaque of Children

et al. 2004

View full text Add to dashboard Cite

Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. The purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n ‫؍‬ 65) and from those with gingivitis (group 2; n ‫؍‬ 53). Extracted DNA from plaque was subjected to two rounds of PCR targeting the 16S rRNA gene using both universal primers and species-specific primers. The results were as follows: group 1, P. gingivalis, 49%; A. actinomycetemcomitans, 55%; and T. forsythensis, 65%; group 2, P. gingivalis, 47%; A. actinomycetemcomitans, 59%; and T. forsythensis, 45%. T. forsythensis was detected more frequently in children with no gingivitis than in those with gingivitis (P ‫؍‬ 0.03). There was no significant difference between the two groups with respect to the presence of P. gingivalis or A. actinomycetemcomitans in either group (P > 0.05). Logistic regression analysis revealed that the odds of a patient having gingivitis were 2.3 times greater in the absence of T. forsythensis. In conclusion, the results of this study have shown that the three pathogens can be detected in the dental plaque of healthy children and of those with gingivitis and that T. forsythensis is associated with dental plaque at sites with no gingivitis.

show abstract

“…T. denticola and P. gingivalis are found by immunological reagents to be present in plaque samples taken from periodontally healthy sites in adults (10) and in children (15). This tneans that most plaque samples would be BANApositive if the BANA colorimetric assay were perfortned at maximal sensitivity.…”

mentioning

confidence: 99%

The effect of incubation temperature on the specificity of the BANA (N‐benzoyl‐DL‐arginine‐naphthylamide) test

Feitosa¹,

Amalfitano

Loesche

1993

Oral Microbiology and Immunology

View full text Add to dashboard Cite

The hydrolysis of BANA by subgingival plaque samples is associated with the presence of either Treponema denticola, Porphyromonas gingivalis, and/or Bacteroides forsythus. A protocol in which pure cultures were incubated for 15 min at 55 degrees C detected about 5 x 10(5) CFU of P. gingivalis and 1 x 10(6) CFU of T. denticola. Clinical studies indicated that the BANA test in this configuration will detect about 10(4) organisms in vivo as compared with the 10(5) to 10(6) organisms found with in vitro grown cells. The BANA test can be made less sensitive by decreasing the time and/or temperature of incubation, which could improve the specificity of the test. In the present study we determined the incubation parameters that would give optimal specificity when the plaque samples were removed from sites of gingival health. Twenty-six approximal plaque samples were taken from each of 90 clinically healthy subjects and incubated with the BANA substrate on PerioScan cards (Oral-B Laboratories) for 5 and 15 min at 35 degrees, 45 degrees, and 55 degrees C. Subjects were randomly assigned to the various temperatures. Wooden toothpicks were inserted interproximally in all sites anterior to distal of the first molars and then each side of the toothpick was wiped onto the PerioScan card. The specificity of the BANA test relative to clinical health was 96% when the cards were incubated for 5 min at 35 degrees C, but decreased to 50-70% when the cards were incubated for 15 min at 35 degrees C or for 5 and 15 min at 45 degrees C and 55 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays

Cited by 30 publications

References 30 publications

Prevalence of Treponema denticola and Porphyromonas gingivalis in plaque from periodontally-healthy and periodontally-diseased sites

Prevalence of Treponema denticola and Porphyromonas gingivalis in plaque from periodontally-healthy and periodontally-diseased sites

Prevalence of Periodontal Pathogens in Dental Plaque of Children

The effect of incubation temperature on the specificity of the BANA (N‐benzoyl‐DL‐arginine‐naphthylamide) test

Contact Info

Product

Resources

About