Detection of Tulip Breaking Virus and Lily Symptomless Virus in Lily Bulbs by Means of Elisa.

Schadewijk, A.R. van

doi:10.17660/actahortic.1986.177.16

Cited by 14 publications

(8 citation statements)

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“…Only two plants, one of 'Apeldoorn' and one of 'Lustige Witwe' were definitely infected by aphids as shown in testing leaf and bulb material by EL-ISA in 1985 and1986. …”

Section: Results Of the Aphid Inoculationsmentioning

confidence: 96%

“…Cultural control by roguing and frequent application of mineral oil and insecticides is expensive, time-consuming, and not always effective. Serological screening of commercial stocks and subsequent elimination of infected propagation material from the market proved to be a successful control strategy in the Netherlands (Van Schadewijk, 1986). This procedure reduces the number of infected bulbs, but does not alter the susceptibility to infection.…”

Section: Introductionmentioning

confidence: 99%

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Determining levels of resistance to Tulip Breaking Virus (TBV) in tulip (Tulipa L.) cultivars

Romanow

Eijk²,

Eikelboom³

et al. 1990

Euphytica

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“…Only two plants, one of 'Apeldoorn' and one of 'Lustige Witwe' were definitely infected by aphids as shown in testing leaf and bulb material by EL-ISA in 1985 and1986. …”

Section: Results Of the Aphid Inoculationsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Determining levels of resistance to Tulip Breaking Virus (TBV) in tulip (Tulipa L.) cultivars

Romanow

Eijk²,

Eikelboom³

et al. 1990

Euphytica

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“…Therefore, the routine testing for TRV of bulbs during storage is not as obvious as has been exemplified for other viruses, e.g., TBV in tulip, and TBV and LSV in lily (Van Schadewijk and Eggink, 1984;Van Schadewijk, 1986).…”

Section: Discussionmentioning

confidence: 99%

Detection of tobacco rattle virus by ELISA and test plants in main sprouts of tulip bulbs during storage at different temperatures

Asjes¹,

Schadewijk

Segers³

1992

Netherlands Journal of Plant Pathology

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The detectability of tobacco rattle virus (TRV) in the main sprouts of primarily and secondarily infected tulip bulbs of cv. Apeldoorn stored at different temperatures from the lifting in July up to February is described. Detection by ELISA was not affected by the size of the main sprout, nor by the size of the bulbs. The rates of TRV-infected bulbs found by ELISA were highest during storage at 13, 9 or 5 ~ continuously, and when temperatures were lowered from 20 or 17 ~ to 5 ~ in October. The percentages detected via test plants, but undetectable by ELISA were also lowest at these temperatures. The unfavourable effect of continuous storage at 20, 17, or 2 ~ as expressed in low ELISA absorbances not significantly different from the mean value of healthy bulbs, was largely overcome during long storage by the change of temperature down to 5 ~ from 20 and 17 ~ or upwards from 2 ~ The reverse from 5 ~ upwards to 17 and 20 ~ affected the detectability by ELISA unfavourably. The rate of detection via test plants in the main sprout and in the small sprouts from different positions in bulbs was only possible at low percentages.The effect of some factors, like different temperatures during storage, detectability of different TRV serotypes, interference of irregular occurrence of TRV in the removed scale and basal-plate tissue with the main sprout, and variable recurrence of TRV in progeny bulbs, is discussed in view of its impact on routine testing of bulbs during storage.

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“…It is possible to detect all three viruses, even if present at low concentrations, through Enzyme Linked Immunosorbent Assay (ELISA) as good antisera are available (Derks & Vink-van den Abeele 1980;Derks et al 1982Derks et al , 1986. As a rule, the starting material is indexed first by testing at least two scales per bulb ( Van Schadewijk 1986). As soon as the in vitro bulblets grown from the meristems are adequately developed, the in vitro material is tested for the virus (or the viruses) present in the starting material.…”

Section: Virus Damagementioning

confidence: 99%

“…Testing ofvirus in the in vitro material is not quite reliable for LSV and not at all reliable for TBV, thus the third indexing ofleaf material grown in the glasshouse is absolutely necessary. Prior to virus testing, the starting material should be stored for 3 weeks at about 2°C to yield reliable results ( Van Schadewijk 1986). Longiflorum spp., however, require storage at 20°C for detection ofTBV (Asjes et af.…”

Section: Virus Damagementioning

confidence: 99%

Production of high quality, healthy ornamental crops through meristem culture

Zaayen¹,

Eijk²,

Versluijs³

1992

Acta Botanica Neerlandica

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An increasing number of ornamental crops are being multiplied in vitro. In this article, the application of meristern culture to plants free from viruses is reviewed and is shown to be useful and cost-effective. First, a literature survey is given on results ofmeristem culture applied to ornamentals and on damage due to virus diseases. Secondly trials and results with lily, Aistroemeria and Delphinium and their viruses are discussed.Virus-free plants have a considerable surplus value compared with infected plants. Additional advantages are that a virus-free crop cannot be a source of contamination for other varieties of the same crop, and that there are no phytosanitary limitations. Virus-free does not mean resistant, however, and re-infection is possible, but can be prevented by strict measures.Meristem culture applied to in vitro cultures oflily, Alstroemeria and Delphinium always yielded a much lower amount of virus-free plantlets than the usual procedure, i.e. meristem culture applied to plants grown in the glasshouse.

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Detection of Tulip Breaking Virus and Lily Symptomless Virus in Lily Bulbs by Means of Elisa.

Cited by 14 publications

References 0 publications

Determining levels of resistance to Tulip Breaking Virus (TBV) in tulip (Tulipa L.) cultivars

Determining levels of resistance to Tulip Breaking Virus (TBV) in tulip (Tulipa L.) cultivars

Detection of tobacco rattle virus by ELISA and test plants in main sprouts of tulip bulbs during storage at different temperatures

Production of high quality, healthy ornamental crops through meristem culture

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