Detection of tuberculosis in cynomolgus macaques (Macaca fascicularis) using a supplementary Monkey Interferon Gamma Releasing Assay (mIGRA)

Warit, Saradee; Billamas, Pamaree; Makhao, Nampueng; Jaitrong, Sarinya; Juthayothin, Tada; Yindeeyoungyeon, Wandee; Dokladda, Kanchana; Smittipat, Nat; Kemthong, Taratorn; Meesawat, Suthirote; Kongsombat, N; Kraitat, C; Prammananan, Therdsak; Palaga, Tanapat; Chaiprasert, Angkana; Malaivijitnond, Suchinda

doi:10.1038/s41598-020-73655-3

Cited by 15 publications

(26 citation statements)

References 32 publications

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“…Similar to the situation with the PCR assay, appropriate samples may be difficult to obtain. Published studies 9,10,18,20 have shown that MTBC-infected NHPs are not always culture positive.…”

Section: Discussionmentioning

confidence: 99%

“…Samples were collected from both cagemates of monkeys that died from TB approximately 6 months prior and from animals housed in neighboring and more distant enclosures. 18 Samples for GIFT assay were collected in June 2018. Overall, 48 samples were nonreactive and interpreted as negative; 11 samples were reactive and included 8 positive (bovine PPD greater than avian PPD) and 3 indeterminate (avian PPD greater than or equal to bovine PPD) interpretations.…”

Section: Case Study 3: Wild-caught Cynomolgus Macaquesmentioning

confidence: 99%

“…Researchers at the National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency in Thailand previously tested a subset of samples from the same animals in February 2018 using the IGRA-Bio-Tech-v1 developed by their Industrial Tuberculosis Team and also subsequently in August 2018 using a new, higher dilution version of the IGRA-BioTech-v2. 18 The IGRA and TST reactivity for the 11 GIFTreactive samples in June 2018 along with available earlier (February) and later (August) IGRA-BioTech results and parallel TST reactivity from the same animals were summarized (Figure 1).…”

Section: Case Study 3: Wild-caught Cynomolgus Macaquesmentioning

confidence: 99%

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Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay

Yee

Prongay

Rompay

et al. 2022

ajvr

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OBJECTIVE To develop a testing algorithm that incorporates multiple assays to evaluate host cellular and humoral immunity and antigen detection concerning Mycobacterium tuberculosis complex (MTBC) infection in captive nonhuman primates. ANIMALS Cohorts of captive-bred and wild-caught macaques from 5 different geographic regions. PROCEDURES Macaques were tested for MTBC infection by use of a γ interferon tuberculosis (GIFT) assay, an interferon-γ release assay, and other assays. In the first 2 cohorts (n = 15 and 181), initial validation of the GIFT assay was performed by use of experimentally infected and unexposed control macaques. In the next 3 cohorts (n = 59, 42, and 11), results were obtained for opportunistically collected samples from macaques exposed during spontaneous outbreaks. RESULTS Sensitivity and specificity of the GIFT assay in the control cohorts were 100% and 97%, respectively, and were variable but enhanced by incorporating results from multiple assays in spontaneous outbreaks. CLINICAL RELEVANCE The detection and management of MTBC infection in captive nonhuman primate populations is an ongoing challenge, especially with animal imports and transfers. Despite standardized practices of initial quarantine with regular intradermal tuberculin skin testing, spontaneous outbreaks continue to be reported. Since infection encompasses a range of disease manifestations over time, a testing algorithm that incorporates multiple assays, such as the GIFT assay, to evaluate host cellular and humoral immunity in addition to agent detection is needed. Testing a combination of samples from controlled studies and spontaneous outbreaks of MTBC infection in nonhuman primates would advance the development and validation of a functional algorithm that incorporates promising tools such as the GIFT assay.

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Section: Discussionmentioning

confidence: 99%

Section: Case Study 3: Wild-caught Cynomolgus Macaquesmentioning

confidence: 99%

Section: Case Study 3: Wild-caught Cynomolgus Macaquesmentioning

confidence: 99%

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Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay

Yee

Prongay

Rompay

et al. 2022

ajvr

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“…caprae [ 13 ]. Additionally, wild animals such as deer and monkeys are also susceptible to these three MTBC members [ 14 , 15 ]. Therefore, OIE added the infection of bovids, cervids, goats, and New World camelids by M .…”

Section: Introductionmentioning

confidence: 99%

Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA

Sun

Chen

et al. 2021

PLoS ONE

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Tuberculosis (TB), a contagious disease mainly caused by Mycobacterium tuberculosis (M. tb), Mycobacterium bovis (M. bovis), and Mycobacterium caprae (M. caprae), poses a major global threat to the health of humans and many species of animals. Developing an ante-mortem detection technique for different species would be of significance in improving the surveillance employing a One Health strategy. To achieve this goal, a universal indirect ELISA was established for serologically detecting Mycobacterium tuberculosis complex infection for multiple live hosts by using a fusion protein of MPB70, MPB83, ESAT6, and CFP10 common in M. tb, M. bovis, and M. caprae as the coating antigen (MMEC) and HRP-labeled fusion protein A and G as a secondary antibody. After testing the known positive and negative sera, the receiver operating characteristic curves were constructed to decide the cut-off values. Then, the diagnostic sensitivity and specificity of MMEC/AG-iELISA were determined as 100.00% (95% CI: 96.90%, 100.00%) and 100.00% (95% CI: 98.44%, 100.00%) for M. bovis infection of cattle, 100.00% (95% CI: 95.00%, 100.00%) and 100.0% (95% CI: 96.80%, 100.00%) for M. bovis infection of sheep, 90.74% (95% CI: 80.09%, 95.98%) and 98.63% (95% CI: 95.14%, 99.76%) for M. bovis infection of cervids, 100.00% (95% CI: 15.81%, 100.00%) and 98.81% (95% CI: 93.54%, 99.97%) for M. bovis infection of monkeys, 100.00% (95% CI: 86.82%, 100.00%) and 94.85% (95% CI: 91.22%, 97.03%) for M. tb infection of humans. Furthermore, this MMEC/AG-iELISA likely detects M. caprae infection in roe deer. Thus this method has a promising application in serological TB surveillance for multiple animal species thereby providing evidence for taking further action in TB control.

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“…Due to the high genetic similarity with humans, NHPs accurately model all TB aspects and are considered the gold standard biomodel in experimental M. tuberculosis infection (Foreman et al, 2017). Infections in captive nonhuman primates by MTBC mainly occur due to direct contact with TB-infected humans through the inhalation of aerosolized bacteria (Ghodbane and Drancourt, 2013;Mätz-Rensing et al, 2015;Warit et al, 2020). Captive nonhuman primates develop an infection clinically indistinguishable from human tuberculosis (Kaushal et al, 2012;Kanabalan et al, 2021), and they recapitulate the full spectrum of infection outcome and pathology seen in humans (Scanga and Flynn, 2014).…”

Section: Introductionmentioning

confidence: 99%

Pulmonary granuloma is not always the tuberculosis hallmark: pathological findings of different tuberculosis stages in New and Old World Nonhuman Primates naturally infected with the Mycobacterium tuberculosis Complex

Pereira¹,

Lopes²,

Pissinatti³

et al. 2021

Preprint

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Herein we present the pathological findings of different tuberculosis stages in Old and New World monkeys kept under human care in Rio de Janeiro, Brazil and naturally infected with Mycobacterium tuberculosis Complex. Fifteen nonhuman primates from five different colonies were incorporated into the study. There are 60% (9/15) Old World Monkeys and 40% (6/15) New World Monkeys. According to the gross and histopathologic findings, the lesions in nonhuman primates of this study are classified into the chronic-active, extrapulmonary, early-activation or latent-reactivation tuberculosis stage. Among the Old World Monkey, 66.7% (6/9) of nonhuman primates, all rhesus monkeys (Macaca mulatta), showed severe granulomatous pneumonia. In all Old World Monkeys cases, typical granulomas were seen in at least one organ regardless of the stage of the disease. In the New World Monkeys, the typical pulmonary granulomas were seen in 16.7% (1/6) of the cases, just in the latent-reactivation stage in Uta Hick’s Bearded Saki (Chiropotes utahickae). In this study, 66.7% (6/9) of Old World Monkeys (OWM) and 83.3% (5/6) of New World Monkeys (NWM) showed pulmonary changes at the histological evaluation. The tuberculosis diagnosis in the nonhuman primates in this study was based on pathological, immunohistochemical, molecular, and bacteriological culture. Although the typical presentation was observed in some cases, the absence of pulmonary granuloma did not exclude the tuberculosis occurrence in nonhuman primates of the Old and New World. Tuberculosis should be included as a cause of interstitial pneumonia with foamy macrophages infiltration in the New World nonhuman primates. Due to the high sensitivity of immunohistochemistry with Anti-Mycobacterium tuberculosis, we suggest the addition of this technique as a diagnostic tool of tuberculosis in the nonhuman primates even when the typical changes are not seen.

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Detection of tuberculosis in cynomolgus macaques (Macaca fascicularis) using a supplementary Monkey Interferon Gamma Releasing Assay (mIGRA)

Cited by 15 publications

References 32 publications

Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay

Tuberculosis detection in nonhuman primates is enhanced by use of testing algorithms that include an interferon-γ release assay

Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA

Pulmonary granuloma is not always the tuberculosis hallmark: pathological findings of different tuberculosis stages in New and Old World Nonhuman Primates naturally infected with the Mycobacterium tuberculosis Complex

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