Detection of tubercular antibody and antigen in sera of bone and joint tuberculosis

Pramanik, J.; Lodam, A. N.; Badole, CM; Reddy, M. V. R.; Patond, Kisan R.; Harinath, B. C.

doi:10.1007/bf02873543

Cited by 1 publication

(2 citation statements)

References 17 publications

(6 reference statements)

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“…Previously, Ag60, Antigen 6 (MPT64) and trichloroacetic acid (TCA) solubilized excretory–secretory (ES) antigen were utilized as serological biomarkers to detect circulating antibodies in sera of OATB patients by ELISA (Pramanik et al . 2000). Also, free TCA solubilized ES antigen was detected in sera of OATB patients by ELISA (Pramanik et al .…”

Section: Introductionmentioning

confidence: 99%

“…Also, free TCA solubilized ES antigen was detected in sera of OATB patients by ELISA (Pramanik et al . 2000). Indeed, Ag85 complex (Ag85, 30–31·5 kDa) is the most abundant secreted protein (60%) of mycobacterial culture filtrate, although a proportion of them remains cell surface associated, which comprises three paralogous proteins, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Diagnosis of osteoarticular tuberculosis by immuno‐PCR assay based on mycobacterial antigen 85 complex detection

Khan

Singh

Sharma

et al. 2021

Letters Applied Microbiology

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Diagnosis of osteoarticular tuberculosis (OATB) exhibits serious challenges owing to paucibacillary nature of specimens and localization of disease at sites that are difficult to access. We recently developed indirect immuno‐PCR (I‐PCR) and real‐time I‐PCR (RT‐I‐PCR) assays for the detection of mycobacterial antigen 85 complex (Ag85) in OATB patients. Detection limits for the purified Ag85 protein were found to be 1 and 41 fg ml−1 by I‐PCR and RT‐I‐PCR, respectively, which were at least 105‐fold lower than respective ELISA. While spiking synovial fluids of non‐TB control subjects with the purified Ag85 protein, LODs of 100 and 120 fg ml−1 were obtained by I‐PCR and RT‐I‐PCR, respectively, thus demonstrating the sample matrix effect. Sensitivities of 87·5 and 70·5% were observed in bodily fluids of confirmed (n = 8) and clinically suspected (n = 51) OATB cases, respectively, by I‐PCR, with a specificity of 93·9% (n = 33). Markedly, the sensitivities obtained by I‐PCR/RT‐I‐PCR were significantly higher (P < 0·05–0·01) than ELISA and GeneXpert assay (n = 30). However, no substantial difference in sensitivity was observed between the I‐PCR and RT‐I‐PCR assays. After further improving the accuracy of I‐PCR, this test may lead to development of an attractive diagnostic kit.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%