Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Christov, Alexander; Dai, Erbin; Liu, Liying; Miller, Leslie W.; Nash, Piers; Lalani, Alshad S.; McFadden, Grant; Nation, P. N.; Tulip, J.; Lucas, Alexandra

doi:10.1002/(sici)1096-9101(1999)24:5<346::aid-lsm5>3.0.co;2-k

Cited by 3 publications

(5 citation statements)

References 44 publications

(51 reference statements)

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“…Another possible area that shows potential is the use of poxviruses as therapeutic oncolytic agents 119 , 120 . The ability of viral proteins to manipulate and dampen inflammatory responses is of interest in many human diseases where inflammation is the major cause of the disorder, such as arthritis and arthrosclerosis 121 , 122 , 123 , 124 , 125 . Poxvirus proteins as immunomodulators have therefore been the target of new drug discovery 126 , 127 .…”

Section: Discussionmentioning

confidence: 99%

“…119,120 The ability of viral proteins to manipulate and dampen inflammatory responses is of interest in many human diseases where inflammation is the major cause of the disorder, such as arthritis and arthrosclerosis. [121][122][123][124][125] Poxvirus proteins as immunomodulators have therefore been the target of new drug discovery. 126,127 One example is the poxvirus-encoded serine-protease inhibitor, Serp-1, that has anti-inflammatory properties and is currently undergoing phase II clinical trials for treatment of vascular inflammation in patients with acute coronary syndrome.…”

Section: Discussionmentioning

confidence: 99%

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Immunopathogenesis of poxvirus infections: forecasting the impending storm

et al. 2007

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Variola virus, the causative agent of smallpox, is a member of the poxvirus family and one of the most virulent human pathogens known. Although smallpox was eradicated almost 30 years ago, it is not understood why the mortality rates associated with the disease were high, why some patients recovered, and what constitutes an effective host response against infection. As variola virus infects only humans, our current understanding of poxvirus infections comes largely from historical clinical data from smallpox patients and from animal studies using closely related viruses such as ectromelia, myxoma and monkeypox. The outcome of an infection is determined by a complex interaction between the type of immune response mounted by the host and by evasion mechanisms that the virus has evolved to subvert it. Disease pathogenesis is also a function of both host and viral factors. Poxviruses are not only cytopathic, causing host tissue damage, but also encode an array of immunomodulatory molecules that affect the severity of disease. The ability of the host to control virus replication is therefore critical in limiting tissue damage. However, in addition to targeting virus, the immune response can inadvertently damage the host to such a degree that it causes illness and even death. There is growing evidence that many of the symptoms associated with serious poxvirus infections are a result of a 'cytokine storm' or sepsis and that this may be the underlying cause of pathology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunopathogenesis of poxvirus infections: forecasting the impending storm

et al. 2007

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“…The high specificity of overall FE intensity, representing different stages of plaque development and disruption, allowed us to apply multiple regression analysis in order to assess potential correlations between FE intensity at selected wavelengths and structural characteristics of unstable plaque formation. We have previously applied this method for the comparison of changes in laser-induced autofluorescence with corresponding structural characteristics after development of transplant vasculopathy in rats (33). In the present study analysis is based on mean fluorescent and histological data in order to confirm our spectroscopic results using the most largely accepted and unquestionable technique for evaluation of atherosclerotic plaque formation (histological analysis).…”

Section: Discussionmentioning

confidence: 91%

“…The mean values of aortic structural characteristics in RVV-treated rabbits' mean values for total plaque area, fatty plaque and thrombosed areas were compared to mean values of fluorescence FE intensity at selected wavelengths by Student's unpaired t test, ANO-VA-factorial and multiple regression analysis as previously described (33). For multiple regression analysis the adjusted R 2 is included to provide a correction for the increase in the R 2 value inherent in this analysis when additional independent variables are assigned to an existing regression by applying a ''penalty'' to the R 2 value based on the numbers of the variables assigned (Abacus Concept, StatView reference.…”

Section: Methodsmentioning

confidence: 99%

“…FE intensity values are assumed to be an integral quantitative measure, directly representing all naturally fluorescing compounds, on the arterial surface layers. Multiple regression analysis was used, as in prior work (33), to detect correlations between plaque composition and FE intensity at multiple wavelengths.…”

Section: Multiple Regression Analysis Of Fe Intensity and Plaque Compmentioning

confidence: 99%

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Optical Detection of Triggered Atherosclerotic Plaque Disruption by Fluorescence Emission Analysis¶

Christov¹,

Dai²,

Drangova³

et al. 2007

Photochemistry and Photobiology

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Fluorescence emission analysis (FEA) has proven to be very sensitive for the detection of elastin, collagen and lipids, which are recognized as the major sources of autofluorescence in vascular tissues. FEA has also been reported to detect venous thromboemboli. In this paper we have tested the hypothesis that FEA can reproducibly detect in vivo and in vitro triggered plaque disruption and thrombosis in a rabbit model. Fluorescence emission (FE) spectra, recorded in vivo, detected Russell's viper venom (RVV)-induced transformation of atherosclerotic plaque. FE intensity at 410-490 nm 4 weeks after angioplasty was significantly lower (P Ͻ 0.0033 by analysis of variance) in RVV-treated rabbits when compared to control animals with stable plaque. FE spectral profile analyses also demonstrated a significant change in curve shape as demonstrated by polynomial regression analysis (R 2 from 0.980 to 0.997). We have also demonstrated an excellent correlation between changes in FE intensity and the structural characteristics detected at different stages of ''unstable atherosclerotic plaque'' development using multiple regression analysis (R 2 ‫؍‬ 0.989). Thus, FEA applied in vivo is a sensitive and highly informative diagnostic technique for detection of triggered atherosclerotic plaque disruption and related structural changes, associated with plaque transformation, in a rabbit model.

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Optical Detection of Triggered Atherosclerotic Plaque Disruption by Fluorescence Emission Analysis¶

Christov¹,

Dai²,

Drangova³

et al. 2000

Photochem Photobiol

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Fluorescence emission analysis (FEA) has proven to be very sensitive for the detection of elastin, collagen and lipids, which are recognized as the major sources of autofluorescence in vascular tissues. FEA has also been reported to detect venous thromboemboli. In this paper we have tested the hypothesis that FEA can reproducibly detect in vivo and in vitro triggered plaque disruption and thrombosis in a rabbit model. Fluorescence emission (FE) spectra, recorded in vivo, detected Russell's viper venom (RVV)-induced transformation of atherosclerotic plaque. FE intensity at 410-490 nm 4 weeks after angioplasty was significantly lower (P < 0.0033 by analysis of variance) in RVV-treated rabbits when compared to control animals with stable plaque. FE spectral profile analyses also demonstrated a significant change in curve shape as demonstrated by polynomial regression analysis (R2 from 0.980 to 0.997). We have also demonstrated an excellent correlation between changes in FE intensity and the structural characteristics detected at different stages of "unstable atherosclerotic plaque" development using multiple regression analysis (R2 = 0.989). Thus, FEA applied in vivo is a sensitive and highly informative diagnostic technique for detection of triggered atherosclerotic plaque disruption and related structural changes, associated with plaque transformation, in a rabbit model.

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Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis

Cited by 3 publications

References 44 publications

Immunopathogenesis of poxvirus infections: forecasting the impending storm

Immunopathogenesis of poxvirus infections: forecasting the impending storm

Optical Detection of Triggered Atherosclerotic Plaque Disruption by Fluorescence Emission Analysis¶

Optical Detection of Triggered Atherosclerotic Plaque Disruption by Fluorescence Emission Analysis¶

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