Detection of toxoplasmosis in (Free living and Industrial) chicken of Qadisiyah province, Iraq

Abstract

The study aimed to investigate the differences in the genetic tree of types infected (Free living and Industrial chickens) with the Toxoplasma parasite, (brain and liver) collecting samples in Al-Qadisiyah province during the period from October 2021 until the end of March 2022. A total of 90 samples of chicken were collected, 60 samples of brain and 30 samples of liver. The purpose of diagnosis, the impression method was used. It was found that 60 samples of free living chickens were all positive for examination. As for industrial chickens, 20 samples from 30 were positive at rate 88.8%, in rate of (83.3%) 50/60 from the brain and (66.6%) 20/30 from the liver. Nested PCR targeting aspecific region within SAG3 gene technique applied for detect the genotype of T.gondii, the results showed that 30/48 of the chickens (brain and liver) (62.5%) were positive. A genetic match was found between Toxoplasma gondii in chickens SAG3 gene and the gene isolates according to NCBI – BLAST.