Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis

Alfonso, Yenisey; Fraga, Jorge; Jiménez, Narciso; Fonseca, Carlos Alberto da; Dorta‐Contreras, Alberto Juan; Cox, Raymundo; Capó, Virginia; Bandera, Francisco; Pomier, Olga; Ginorio, Dora

doi:10.1016/j.exppara.2009.03.009

Cited by 31 publications

(16 citation statements)

References 31 publications

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“…[7] However, in the developing countries, toxoplasmosis remains the most important cause of focal brain lesions[8] and in India, it is a close second to tuberculosis at times causing diagnostic difficulties. [910] This is particularly relevant considering the high seroprevalence of IgG antibodies to T. gondii in South Indian healthy voluntary blood donors (20.3%).…”

Section: Discussionmentioning

confidence: 99%

Utility of molecular and serodiagnostic tools in cerebral toxoplasmosis with and without tuberculous meningitis in AIDS patients: A study from South India

Adurthi

Mahadevan

Bantwal

et al. 2010

Ann Indian Acad Neurol

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Background:Antemortem diagnosis of cerebral toxoplasmosis, the second most common opportunistic infection (OI) in HIV-infected individuals in developing countries is a challenge.Materials and Methods:Toxoplasma gondii (T.gondii) -specific serology and nested polymerase chain reaction (nPCR) were evaluated in sera and ventricular/lumbar cerebrospinal fluid (CSF) of 22 autopsy confirmed cases of cerebral toxoplasmosis with HIV and 17 controls. Frequency of concomitant T.gondii infection was investigated in 17 cases of HIV-associated tuberculous meningitis (TBM).Results:The sensitivity, specificity, and positive and negative predictive values of T. gondii IgG on CSF (ventricular and lumbar) and sera was 100% in histology proven cerebral toxoplasmosis (concentrations: 258 ± 50, 231 ± 36, and 646 ± 243 IU/mL, respectively); majority (94%) being high avidity type, suggesting reactivation/reinfection. The sensitivity of B1 nPCR was 100% on ventricular CSF, whereas it was only 77% on lumbar CSF. Based on histology, nPCR, and IgG serology, T. gondii co-infection with TBM was observed in 65% (11/17) of cases.Discussion and Conclusion:CSF IgG serology and nPCR are tests with high sensitivity and specificity for the diagnosis of cerebral toxoplasmosis. TBM and cerebral toxoplasmosis can coexist and should be considered in the background of HIV infection in developing countries.

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Section: Discussionmentioning

confidence: 99%

Utility of molecular and serodiagnostic tools in cerebral toxoplasmosis with and without tuberculous meningitis in AIDS patients: A study from South India

Adurthi

Mahadevan

Bantwal

et al. 2010

Ann Indian Acad Neurol

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“…PCR is highly specific, but in some cases, it is difficult to perform DNA extraction and to achieve the desired concentrations of samples, which limits the sensitivity of the technique (ALFONSO et al, 2009). Moreover, cysts are randomly distributed in the host tissues, and their concentration is lowered depending on the volume or the number of samples to be extracted (PIERGILI, 2004).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil

et al. 2016

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Infections caused by protozoa belonging to the (17/80,.2) of the tested samples and T. gondii DNA in 15% (12/80,. Moreover, 6.2% (5/80,) samples contained DNA of both protozoan. The presence of N. caninum nucleic acids was not observed in tested samples. However, 21,2% (17/80, IC: 12,2) das amostras testadas e DNA de T. gondii em 15% (12/80, IC: 7,8). Em 6,2% (5/80, IC: 2,[1][2][3][4][5][6][7][8][9][10][11][12][13]9), foram detectados DNA de ambos os protozoários. Todas as amostras positivas no PCR (23, IC: 14,1)

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“…Early detection of Toxoplasma gondii or its shedding oocysts is necessary to be performed to control this disease. Several methods were developed to diagnose the toxoplasmosis, for example, histology is usually utilized to detect Toxoplasma gondii but sparse spread in tissue make it difficult to be detected; Bioassays are highly sensitive and specific but it is expensive and labour intensive; PCR detection [4,5] of Toxoplasma gondii DNA also necessitates invasive sampling techniques or necropsy. Serology is easy to detect Toxoplasma gondii but cross-reactive antibodies infected with related coccidian parasites may give false positive results [6].…”

Section: Introductionmentioning

confidence: 99%

Detection of Toxoplasma gondii with a DNA molecular beacon probe

Zhou¹,

Xu²,

Yang³

et al. 2009

SPIE Proceedings

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Toxoplasma gondii is a kind of microscopic parasite that may infect humans, and there are increasing concerns on the early detection of latent Toxoplasma gondii infection in recent years. This research highlights a new type of molecular beacon (MB) fluorescent probe for Toxoplasma DNA testing. We combined high-efficiency fluorescent inorganic core-shell quantum dots-CdTe/ZnS (as fluorescent energy donor) and BHQ-2 (energy acceptor) to the single-strand DNA of Toxoplasma Gondii, and a molecular beacon sensing system based on fluorescence resonance energy transfer (FRET) was achieved. Core-shell quantum dots CdTe/ZnS was firstly prepared in aqueous solution, and the influencing factor of its fluorescent properties, including CdTe/Na 2 S/Zn(CH 3 COO) 2 (v/v), dependence of reaction time, temperature, and pH, is investigated systematically. The synthesized quantum dots and molecular beacon were characterized by transmission electron microscopy (TEM), ultraviolet-visible spectrophotometer (UV-vis), fluorescent spectrophotometer (FS), respectively. The TEM results showed that CdTe/ZnS core-shell quantum dots is ~11nm in size, and the quantum dots is water-soluble well. The sensing ability of target DNA of assembled MB was investigated, and results showed that the target Toxoplasma Gonddi DNA can be successfully detected by measuring the change of fluorescence intensity. The results showed that the current sensing probe will be a useful and convenient tool in Toxoplasma gondii early detection.

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Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by nested PCR and rapid identification of type I allele at B1 gene by RFLP analysis

Cited by 31 publications

References 31 publications

Utility of molecular and serodiagnostic tools in cerebral toxoplasmosis with and without tuberculous meningitis in AIDS patients: A study from South India

Utility of molecular and serodiagnostic tools in cerebral toxoplasmosis with and without tuberculous meningitis in AIDS patients: A study from South India

Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil

Detection of Toxoplasma gondii with a DNA molecular beacon probe

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