Detection of Toxoplasma gondii in 94 placentae from infected women by polymerase chain reaction, in vivo, and in vitro cultures

Fricker‐Hidalgo, Hélène; Pelloux, Hervé; Racinet, C; Grefenstette, I.; Bost-Bru, C.; Goullier-Fleuret, A.; Ambroise-Thomas, P

doi:10.1016/s0143-4004(98)91049-9

Cited by 56 publications

(31 citation statements)

References 15 publications

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“…At birth, the sensitivity of Toxoplasma detection is poor, ranging from 25 to 60.9% (7,16). The use of immunologic methods is thus crucial for definitive postnatal diagnosis of CT. IgG is known to cross the placental barrier readily, but IgM and IgA may "leak" through the placenta during labor and contaminate the newborn's blood.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are two major obstacles to postnatal diagnosis, namely, the poor sensitivity of Toxoplasma detection (7,16) (27,28), are claimed to distinguish maternal from fetal or neonatal neosynthesized antibodies. In the present collaborative study involving 14 laboratories supported by the European Community Biomed 2 program, we evaluated IB and ELIFA methods for the postnatal diagnosis of CT.…”

Section: In a Study Involving 14 Laboratories Supported By The Europementioning

confidence: 99%

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Strategy for Diagnosis of Congenital Toxoplasmosis: Evaluation of Methods Comparing Mothers and Newborns and Standard Methods for Postnatal Detection of Immunoglobulin G, M, and A Antibodies

et al. 2001

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In a study involving 14 laboratories supported by the European Community Biomed 2 program, we evaluated immunologic methods for the postnatal diagnosis of congenital toxoplasmosis (CT). Among babies born to mothers who seroconverted to positivity for toxoplasmosis during pregnancy, we analyzed 55 babies with CT on the basis of persistent anti-Toxoplasma immunoglobulin G (IgG) at 1 year of life and 50 control babies without anti-Toxoplasma IgG at 1 year of life in the absence of curative treatment with pyrimethaminesulfonamides. We tested in-house methods such as the enzyme-linked immunofiltration assay (ELIFA) or Immunoblotting (IB) for the detection of IgG or IgM; these methods allowed comparison of the immunologic profiles of the mothers and the infants. We compared ELIFA and IB with a commercial enzyme immunoassay (EIA) or in-house immunosorbent agglutination assay (ISAGA) for the detection of IgM or IgA. The performances of combinations of methods were also assessed. A cumulative sensitivity of 98% during a 1-year follow-up was obtained with the ELIFA plus ISAGA combination. Only one case of CT was missed by the ELIFA plus ISAGA combination, whereas three cases were missed by the IB plus ISAGA combination, even though 48% of patients with CT were treated with pyrimethamine-sulfonamides, which are known to inhibit antibody neosynthesis. A similar performance was obtained with either ELIFA or IB in combination with EIA. The difference in performance between ELIFA plus ISAGA and IB plus ISAGA was not statistically significant (P ‫؍‬ 0.31), and we conclude that both combinations of tests can be used for the diagnosis of CT in newborns.

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Section: Discussionmentioning

confidence: 99%

Section: In a Study Involving 14 Laboratories Supported By The Europementioning

confidence: 99%

Strategy for Diagnosis of Congenital Toxoplasmosis: Evaluation of Methods Comparing Mothers and Newborns and Standard Methods for Postnatal Detection of Immunoglobulin G, M, and A Antibodies

et al. 2001

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“…However, diagnosis of congenital toxoplasmosis during pregnancy by PCR and/or mouse inoculation usually identifies no more than 60 to 70% of the infected fetuses (13,24,38,39,41). Thus, exploring the antibody response to T. gondii in the newborn child seems an obvious approach to improve the neonatal diagnosis of congenital toxoplasmosis.…”

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confidence: 99%

Use of Recombinant Antigens for Early Postnatal Diagnosis of Congenital Toxoplasmosis

Buffolano

Beghetto²,

Pezzo

et al. 2005

J Clin Microbiol

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The main objective of this work was to improve the early serologic diagnosis of toxoplasmosis in children at risk of congenital infection by using recombinant antigens. Serum samples from 104 infants born to mothers with primary Toxoplasma gondii infection acquired during pregnancy, of which 35 were congenitally infected and 22 had clinical silent toxoplasmosis at birth, were included. Immunoglobulin M (IgM), IgG, and IgG subtype antibodies against epitopes carried by fragments of T. gondii MIC2, MIC3, MIC4, M2AP, AMA1, and SAG1 gene products were measured by performing parallel enzyme immunoassays (Rec-ELISAs). Recombinant antigens preferentially reacted with IgG antibodies from infected infants compared to uninfected subjects (P < 0.0001), indicating that sera from infected children recognized a more diverse repertoire of antigens than sera transferred over the placenta from the mothers. Using two serial samples collected within 3 months of life, it was possible to demonstrate a neosynthesis of specific anti-MIC2 and anti-SAG1 immunoglobulin G, mainly of the IgG2 subtype, in 13 out of 20 infants with congenital toxoplasmosis. IgM antibodies in 97% of infected infants reacted with at least one of the recombinant antigens, confirming the diagnosis of congenital infection as soon as 2 months after birth (P < 0.0001). The use of recombinant antigens is effective in distinguishing T. gondii-infected from uninfected infants and shows that assays based on recombinant antigens improve the diagnosis of newborns with congenital toxoplasmosis.

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“…In the detection of T. gondii in a range of tissues in sheep and cattle challenged with different doses of sporulated oocysts, the PCR assay was much more sensitive and speci®c than histological assay [16,23]. This greater sensitivity of the PCR has been con®rmed in the diagnosis of congenital toxoplasmosis by many others [24,25].…”

Section: Discussionmentioning

confidence: 98%

Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice

Hafid¹,

Flori²,

Rabérin³

et al. 2001

Journal of Medical Microbiology

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Detection of Toxoplasma gondii in 94 placentae from infected women by polymerase chain reaction, in vivo, and in vitro cultures

Cited by 56 publications

References 15 publications

Strategy for Diagnosis of Congenital Toxoplasmosis: Evaluation of Methods Comparing Mothers and Newborns and Standard Methods for Postnatal Detection of Immunoglobulin G, M, and A Antibodies

Strategy for Diagnosis of Congenital Toxoplasmosis: Evaluation of Methods Comparing Mothers and Newborns and Standard Methods for Postnatal Detection of Immunoglobulin G, M, and A Antibodies

Use of Recombinant Antigens for Early Postnatal Diagnosis of Congenital Toxoplasmosis

Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice

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