Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

Abstract

Aim:The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).Material and Methods:Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.Result:The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.Conclusion:The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.

Pathologic and immunohistochemical findings in an outbreak of systemic toxoplasmosis in a mob of red kangaroos

“…Although the use of PCR has revolutionized infectious disease testing, it is important to highlight that IHC has proven to be a useful tool for the pathologist to diagnose toxoplasmosis in several animal species as well as for screening purposes (e.g., at the slaughterhouse). 21,24,36,38 IHC is also available for use in humans and can be performed easily on different body fluids for the diagnosis of acute toxoplasmosis. 30 To our knowledge, a large panel of antibodies to T. gondii used to identify suitable and specific antibodies for IHC has not been reported previously, nor has the use of monoclonal anti-MIC3 T4 2F3 for IHC procedures been reported.…”

Seroepidemiological study of the exposure to Toxoplasma gondii among horses in Algeria and analysis of risk factors

“…The different ages of animals were pooled into four groups [13], one group with an age between 1 and 5 years old (n=154), the second group with horses between 6 and 10 years old (n=377), the third group with horses between 11 and 15 years old (n=164), and the last group with horses >15 years (41). The horses had different colors: Bay (250), Chestnut (226), Grey (194), Roan (41), Seal brown (11), Chestnut roan (7), Piebald (4), Chestnut dun (2), and Buckskin (1).…”

“…Toxoplasmosis is a worldwide reported zoonotic infection caused by the protozoon Toxoplasma gondii. The obligate intracellular parasite infects a broad range of mammalian and avian hosts including approximately one-third of the human population [1]. In this zoonosis, the definitive host of T. gondii is the cat and related wild felids; these are the only hosts shedding oocysts into the environment to contaminate pastures, food items, and water [2].…”

Cloning and expression of Toxoplasma gondii GRA-4 recombinant protein as a toxoplasmosis diagnostic kit candidate

“…Toxoplasmosis diagnosis consists of either a serological test using monoclonal antibody (fluorescent antibody, ELISA), polymerase chain reaction (PCR), molecular probes (DNA tracking), or dipstick test, in conjunction with clinical symptoms. Toxoplasma antigens have been detected in several domestic cat organs using immunohistochemistry with antigen labeled-(strept) Avidin-Biotin (LAB-SA) [3]. Hanafiah et al [4] also detected 33.3% toxoplasmosis prevalence in cats using the PCR technique.…”