Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

Al-Shwaikh, Rana M. Abdullah; Alornaaouti, Abbas Falih

doi:10.30526/2017.ihsciconf.1767

Cited by 4 publications

(3 citation statements)

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“…The inhibitory effect of Pyocyanin from precipitate and filtrate on the growth of S.aureus indicates the ability of P.aeruginosa to produce Pyocyanin pigment (Qasim et al, 2009). As shown in Figure (7). This result is close to that of (Iwalokun et al, 2006) who obtained 81.5% of their Pyocyanin-producing isolates.…”

Section: Detection the Ability Of P Aeruginosa To Produce Pyocyanin P...supporting

confidence: 91%

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

2022

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The current study included a group of (150) clinical swabs for patients suffering from burns, wounds, diabetic ulcers, and urinary tract infections for (69) males and (81) females, their ages ranging from 1-68 years, for the period of November 2021 until March 2022, from Hospitals in Mosul. 75 samples of P. aeruginosa were isolated, representing (50%) of the total clinical samples, and the rate of isolation of them from samples of burns, wounds, diabetic foot ulcers, and urinary tract infections was 75% (42/56), 41.1% ( 23/56), 53.3% (8/15) and 8.7% (2/23), respectively. The antimicrobial susceptibility test was performed by the disk diffusion method, and it was found that the isolates are multi-antibiotic resistant. The percentage of resistance shown by the isolates was 70.7% for Piperacillin, 89.3% for Amikacin, 74.7% for Aztreonam and Levofloxacin, 81.3% for Gentamicin, Ceftazidime, and Cefepime, 76% for Ciprofloxacin, 80% for Netilmicin and Tobramycin, and 45.3% for Imipenem. The MIC values for the antibiotic Levofloxacin were determined using Macrodilution method, the results showed that the MIC value ranged between (0.5-512) µg/ml.The biofilm formation was assayed by the microtiter plate. Pyocyanin was extracted from the culture supernatants, and the absorbance values were measured using a spectrophotometer.The results showed that the antibiotic Levofloxacin at the MIC and Sub-MIC concentrations has an inhibitory effect to varying degrees on biofilm formation and pyocyanin pigment production.

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Section: Detection the Ability Of P Aeruginosa To Produce Pyocyanin P...supporting

confidence: 91%

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

2022

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“…The results of the current study were consistent with the results of (17). Whereas, P. aeruginosa isolates showed high resistance to Ceftazidime (81%), Cefotaxime (78%), Piperacillin (76%) and were resistant to Ciprofloxacin, Tobramycin (74%) and Genamicin (72%).…”

Section: 2supporting

confidence: 90%

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

2022

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The current study included a group of (150) clinical swabs for patients suffering from burns, infections in wounds, diabetic foot ulcers and urinary tract infections for both gender, (69) males and (81) females, their ages ranged between 1-68 years, for the period of November 2021 until the end of February 2022, from Hospitals in Mosul. After the final diagnosis of the samples, (75) isolates of P. aeruginosa were obtained, representing (50%) of the total clinical samples, and the rate of isolation of them from samples of burns, wounds, diabetic foot ulcers and urinary tract infections was 75% (42/56), 41.1% ( 23/56), 53.3% (8/15) and 8.7% (2/23), respectively.The antimicrobial susceptibility test was performed by the disk diffusion method, and it was found that the isolates are multi-antibiotic resistant. The percentage of resistance shown by the isolates were 70.7% for Piperacillin , 89.3% for Amikacin, 74.7% for Aztreonam and Levofloxacin , 81.3% for Gentamicin, Ceftazidime and Cefepime, 76% for Ciprofloxacin, 80% for Netilmicin and Tobramycin , and 45.3% for Imipenem.The effect of the antibiotic Levofloxacin on the PslA gene responsible for the production of biofilms, and the PhzM gene responsible for the production of the pyocyanin pigment by bacteria was studied by extracting the DNA of ten bacterial isolates and then performing a polymerase chain reaction (PCR) using the primers PslA, PhzM, and then performing the electrophoresis process For the final reaction products and to note the presence of Bands before and after the treatment of bacterial isolates with the antibiotic.Five isolates (PA1, PA4, PA5, PA6, PA7) were selected to perform DNA sequencing before and after treatment with the antibiotic Levofloxacin by sending the PCR products of the five samples with the 16SrRNA primer to the Macrogen biotechnology company to know the sequence of nitrogenous bases using a Genetic analyzer 3130 , Changes were observed in the sequence of nitrogenous bases after treatment with the antibiotic Levofloxacin, four new genes were recorded in the National Center for Biotechnology Information (NCBI), and these genes were named short for the name of the supervisor and researcher.

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“…The mexR gene was also detected in 100 % isolates in the current study, which was in line with literature. 22 The current findings showed that some clinical specimens had a greater relative frequency of virulence genes. It has been hypothesised that the location of the infection and the duration of the illness can result in the development of some virulence determinants, which in turn affects how virulent P. aeruginosa clinical isolates are.…”

Section: Discussionmentioning

confidence: 52%

Molecular detection of virulence genes in pseudomonas aeruginosa isolated from different clinical infections

Enass Abdul Kadhum Almkhadhree,

Kawther Adnan Alqaseer,

Khairi Jameel AL Ruaby

2023

J Pak Med Assoc

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Objective: To isolate pseudomonas aeruginosa from different clinical specimens, and to detect virulence factors.Method: The cross-sectional study was conducted at Al-Zharr Hospital and private clinics in Kut city of Wasit provincein Iraq from October 15, 2021, to April 30, 2022, and comprised samples obtained from different patient sites, such aswound swabs, ear swabs, urine samples, burn swabs and respiratory tract swabs. The samples were subjected tomolecular testing using, among other tools, polymerase chain reaction. The isolates were cultured on different media,such as blood agar, MacConkey agar and cetrimide agar, and were further diagnosed by biochemical testing. Datawas analysed using SPSS 20.Results:Of the 212 samples, there were 70(33%) wound swabs, 53(25%) ear swabs, 41(19.3%) urine samples, 33(15.6%)burn swabs and 15(7%) respiratory tract swabs. Pseudomonas aeruginosa was identified in 54(25.47%) isolates viaculture-based characterisation. All 54(100%) isolates were positive for mexR and toxA on polymerase chain reaction,44(81.48%) were positive for exoU gene, 43(79.62%) for exoT gene, 36 (66.66) for exoT gen, 35(64.81%) for plcH, geneand 14(25.92 %) for exoS gene.Conclusions: Wound swabs were found to be the most common site for pseudomonas aeruginosa, followed by earswabs and urine samples.Keywords: Pseudomonas aeruginosa, Virulence, Agar, Cetrimonium, Virulence, Burns, Polymerase chain reaction, DNA.

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Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

Cited by 4 publications

References 0 publications

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

Molecular detection of virulence genes in pseudomonas aeruginosa isolated from different clinical infections

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