Detection of total protein in milk using phosphomolybdic acid-mediated surface-enhanced Raman spectroscopy

Abstract

Although many surface‐enhanced Raman scattering (SERS)‐based methods for detecting specific proteins have been studied, simple and direct detection of total protein in liquid using a SERS‐based method remains difficult. In this study, a distinguishable effect on the SERS spectra from pre‐mixture of phosphomolybdic acid (PMA) with protein was found, indicating that PMA could be used as a SERS reporter for total protein detection in a liquid sample. Further experiments confirmed a good linear relationship between a premixed concentration of protein (casein, whey protein or bovine serum albumin) and the SERS intensity of PMA in our SERS system. Using casein as a reference, a PMA‐mediated SERS method was proposed that can quantitatively analyze protein at 2.5–25 µg/ml with a limit of detection of 1.5 µg/ml. Our PMA‐mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk. Copyright © 2015 John Wiley & Sons, Ltd.

Cluster-Level Heterostructure of PMo12/Cu for Efficient and Selective Electrocatalytic Hydrogenation of High-Concentration 5-Hydroxymethylfurfural

“…PMo 12 exhibits characteristic P−O, Mo�O, and Mo−O−Mo vibration peaks at 1057, 953, and 882 cm −1 in the Fourier transform infrared (FT-IR) spectrum (Figure 2a), which were observed in PMo 12 /Cu, indicating the preservation of the PMo 12 structure. 16 18 which is accompanied by the formation of a new peak of Mo−O−Cu, consistent with the previous literature reports 19 but distinct from that of MoO 3 (Figure S5). These findings indicate that the structure of PMo 12 clusters remains intact with the formation of anchoring bonding of Mo−O−Cu with the surface Cu atoms.…”

Recent advances in linear and non‐linear Raman spectroscopy. Part XI

“…Huang and co‐workers reported the detection of total protein in milk using phosphomolybdic acid‐mediated surface‐enhanced Raman spectroscopy. They claim that their PMA‐mediated SERS method is a simple and rapid method for quantitative analysis of total protein in milk . Ji, Zhao, and Ozaki reviewed the use of SERS in the analysis of semiconductor materials.…”

Fabrication of High-Stability and -Sensitivity Perovskite Nanoparticles with a Core–Shell Structure for Surface-Enhanced Raman Scattering

“…Raman spectroscopy is a noninvasive analytical technique that relies on analyzing scattered light to characterize the structure and chemical composition of materials with dependable accuracy. − Raman spectroscopy has been widely utilized in the biomedical field as a nondestructive, label-free technique. Its integration with other imaging technologies, such as optical microscopy and thermal imaging, has enabled multimodal imaging, which has led to significant applications in live cell imaging, − single-molecule detection, bioengineering, viral diagnostics, − and drug discovery , and screening, − highlighting its versatility and the ongoing expansion of its capabilities.…”