Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer

Sun, Qipeng; Li, Liao-Yuan; Chen, Zhong; Pang, Jun; Yang, Wei-Jiao; Zhou, Xiangfu; Qiu, Jianguang; Su, Ziyi; He, Dan; Gao, Xin

doi:10.2353/jmoldx.2010.100002

Cited by 26 publications

(32 citation statements)

References 24 publications

(25 reference statements)

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“…Our group has previously reported an initial scoring system for assessing ERG rearrangements in biopsy samples, based on the use of fluorescence in situ hybridisation (FISH), for the diagnosis of PCa and the risk assessment of lymph node metastasis. This proposed system has demonstrated excellent sensitivity and specificity (16,17). In clinical practice, an increase in ERG rearrangements was observed to be associated with more aggressive characteristics.…”

Section: Introductionmentioning

confidence: 97%

“…Finally, 306 cases with clinically localised PCa were enrolled in this retrospective analysis. The diagnosis of PCa was confirmed via transrectal ultrasound (TRUS)-guided needle biopsy preoperatively (median total biopsy cores, 12; range, [10][11][12][13][14][15][16]. This study was approved by the Institutional Ethics Committee of the Third Affiliated Hospital of Sun Yat-Sen University, and all patients signed informed consent forms prior to the intervention.…”

Section: Methodsmentioning

confidence: 99%

“…The selection of slides for FISH analysis was performed by the pathologist conducting the diagnosis. The ERG rearrangement was calibrated with a dual-colour break-apart FISH assay (Beijing GP Medical Technologies, Ltd., Beijing, China) as previously described (16)(17)(18).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent to washing in deionised water for 5 min, the sections were boiled in deionised water at 100˚C for 27 min and then digested with Proteinase K (Beijing GP Medical Technologies, Ltd.) at 37˚C for 10 min. The sections on the slides were then dried, and hybridisation was performed as described previously (16,17). Next, the slides were counterstained and mounted with DAPI, examined under an oil objective at 100x magnification using an Olympus fluorescence microscope (BX51; Olympus Corp., Tokyo, Japan) and imaged with a CCD camera (DP70; Olympus Corp.) using the PathFinder CellScan software system (IMSTAR S.A., Paris, France).…”

Section: Assessment Of Erg Rearrangements Via Fishmentioning

confidence: 99%

“…ERG rearrangement rate in the patient was calculated using the following formula: ERG rearrangement rate (%) = number of cells exhibiting an abnormal signal pattern/number of cancer cells (16,17). All slices were independently assessed by three experienced researchers (Dr Li Lu, Dr Hao Zhang and Dr Guo-Liang Hou) who were blinded to the clinicopathological parameters, and any discordant results were reassessed until a consensus was achieved ( Fig.…”

Section: Assessment Of Erg Rearrangements Via Fishmentioning

confidence: 99%

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ERG rearrangement as a novel marker for predicting the extra-prostatic extension of clinically localised prostate cancer

Zhang

Pang

et al. 2016

Oncology Letters

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Abstract. Currently, there are no well-established preoperative clinicopathological parameters for predicting extra-prostatic extension (EPE) in patients with clinically localised prostate cancer (PCa). The transmembrane protease serine 2 (TMPRSS2)-ETS-related gene (ERG) fusion gene is a specific biomarker of PCa and is considered a prognostic predictor. The aim of the present study was to assess the value of this marker for predicting EPE in patients with clinically localised PCa. In total, 306 PCa patients with clinically localised disease, including 220 patients (71.9%) with organ-confined disease and 86 EPE cases (28.1%), were included in the study. Receiver operating characteristic curves and logistic regression were employed to establish the optimal cut-off value and to investigate whether ERG rearrangement was an independent predictor for the EPE of clinically localised PCa. A leave-one-out cross-validation (LOOCV) model was implemented to validate the predictive power of ERG rearrangement. An increase in ERG rearrangements was identified to be associate`d with EPE, and the optimal cut-off for predicting EPE was determined to be 2.25%, with a sensitivity of 70.24% [95% confidence interval (CI), 62.6-78.9%], a specificity of 80. 43% (95% CI,, and an area under the curve (AUC) of 0.781 (95% CI,. In the LOOCV model, ERG rearrangement also demonstrated good performance for predicting EPE (sensitivity, 76.923%; specificity, 71.429%; 95% CI for AUC, 0.724-0.958). In addition, a high Gleason score (≥7) and a cT2c classification upon biopsy were independent factors for EPE.

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Section: Introductionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Assessment Of Erg Rearrangements Via Fishmentioning

confidence: 99%

Section: Assessment Of Erg Rearrangements Via Fishmentioning

confidence: 99%

See 3 more Smart Citations

ERG rearrangement as a novel marker for predicting the extra-prostatic extension of clinically localised prostate cancer

Zhang

Pang

et al. 2016

Oncology Letters

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A meta-analysis of the relationship between glutathione S-transferases gene polymorphism and hepatocellular carcinoma in Asian population

Chen

Peng

et al. 2012

Mol Biol Rep

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The results from the published studies on the association between glutathione S-transferases (GST) gene polymorphism and hepatocellular carcinoma (HCC) in Asian population are still conflicting. GSTM1, GSTT1 and GSTP1 are the mainly mutant sites reported at present. This meta-analysis was performed to evaluate the relationship between GST gene polymorphism and HCC risk in Asians. Association studies were identified from the databases of PubMed, Embase, Cochrane Library and CBM-disc (China Biological Medicine Database) on February 1, 2012, and eligible investigations were synthesized using meta-analysis method. Results were expressed with odds ratios (OR) for dichotomous data, and 95 % confidence intervals (CI) were also calculated. Twenty-five investigations were identified for the analysis of association between polymorphic deletion of GSTM1 and HCC, consisting of 3,547 patients with HCC and 6,132 controls. There was a marked association between GSTM1 null genotype and HCC susceptibility (OR 1.48, 95 % CI 1.19-1.85, P = 0.0004). GSTM1 null genotype was associated with HCC risk in Chinese. Furthermore, null genotype of GSTT1 was associated with HCC susceptibility in Asians. For the GSTM1-GSTT1 interaction analysis, the dual null genotype of GSTM1/GSTT1 was significantly associated with HCC susceptibility in Asian population. However, GSTP1 ile105 val gene polymorphism was not associated with HCC risk in Asian population. In conclusion, GSTM1/GSTT1 null genotype is associated with the HCC susceptibility. However, GSTP1 gene polymorphism is not associated with HCC risk.

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A meta-analysis of the relationship between glutathione S-transferase T1 null/presence gene polymorphism and the risk of lung cancer including 31802 subjects

Zhou

Zheng

et al. 2013

Mol Biol Rep

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The relationship between glutathione S-transferase T1 (GSTT1) null/presence gene polymorphism and the risk of lung cancer from the published reports are still conflicting. This study was conducted to evaluate the relationship between GSTT1 null/presence gene polymorphism and the risk of lung cancer using meta-analysis method. The association studies were identified from PubMed, and Cochrane Library on July 1, 2012, and eligible investigations were included and synthesized using meta-analysis method. 51 reports were recruited into this meta-analysis for the association of null genotype of GSTT1 with lung cancer susceptibility, consisting of 15,140 patients with lung cancer and 16,662 controls. There was a marked association between GSTT1 null genotype and lung cancer risk in overall populations (OR = 1.15, 95 % CI 1.04-1.27, P = 0.007). Furthermore, GSTT1 null genotype was associated with the lung cancer risk in Asians (OR = 1.47, 95 % CI 1.23-1.76, P < 0.0001). However, GSTT1 null genotype was not associated with the risk of lung cancer in Caucasians, Brazilian population and Africans. In conclusion, GSTT1 null genotype is associated with the lung cancer in overall populations and in Asians.

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Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer

Cited by 26 publications

References 24 publications

ERG rearrangement as a novel marker for predicting the extra-prostatic extension of clinically localised prostate cancer

ERG rearrangement as a novel marker for predicting the extra-prostatic extension of clinically localised prostate cancer

A meta-analysis of the relationship between glutathione S-transferases gene polymorphism and hepatocellular carcinoma in Asian population

A meta-analysis of the relationship between glutathione S-transferase T1 null/presence gene polymorphism and the risk of lung cancer including 31802 subjects

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