Detection of three stable genetic clones of CTX-M-15-producing Klebsiella pneumoniae in the Barcelona metropolitan area, Spain

Coelho, Alicia; Mirelis, Beatriz; Alonso-Tarrés, Carles; Larrosa, Nieves; Miró, Elisenda; Abad, Raquel; Bartolomé, Rosa; Castañer, Mireia; Prats, Guillem; Johnson, James R.; Navarro, Ferrán; González-López, Juan José

doi:10.1093/jac/dkp264

Cited by 27 publications

(28 citation statements)

References 7 publications

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“…Pulsotypes VII and VIII, which exhibited 79% similarity, belonged to the same sequence type, i.e. ST-1 [5]. In the present report, we describe the location and genetic organisation of bla CTX-M-15 in these 37 K. pneumoniae isolates.…”

Section: Introductionmentioning

confidence: 65%

“…The 37 studied K. pneumoniae isolates represented all of the CTX-M-15-producing isolates found among 111 ESBL-producing K. pneumoniae collected from three hospitals within metropolitan Barcelona between 2005 and 2008, as previously reported [5]. According to 7-gene MLST, these isolates belonged to sequence types ST-1 (n = 12), ST-14 (n = 1), ST-37 (n = 1), ST-147 (n = 1), ST-321 (n = 1), ST-326 (n = 16) and ST-327 (n = 5) [5].…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…In a recent study [5] investigating the presence of CTX-M-15-producing K. pneumoniae clonal strains in the Barcelona metropolitan area from 2005 to 2008, we screened for bla CTX-M-15 among 111 non-duplicate extended-spectrum ␤-lactamase (ESBL)-producing K. pneumoniae isolates. We reported 37 CTX-M-15-producing K. pneumoniae isolates that, based on pulsed-field gel electrophoresis (PFGE), were classified in eight clonal clusters, corresponding with seven sequence types, as determined by multilocus sequence typing (MLST) [5].…”

Section: Introductionmentioning

confidence: 99%

“…We reported 37 CTX-M-15-producing K. pneumoniae isolates that, based on pulsed-field gel electrophoresis (PFGE), were classified in eight clonal clusters, corresponding with seven sequence types, as determined by multilocus sequence typing (MLST) [5]. Pulsotypes VII and VIII, which exhibited 79% similarity, belonged to the same sequence type, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration

Coelho¹,

González-López²,

Miró³

et al. 2010

International Journal of Antimicrobial Agents

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The localisation and genetic organisation of bla(CTX-M-15) were studied in 37 CTX-M-15-producing Klebsiella pneumoniae isolates collected from 2005 to 2008 within the Barcelona metropolitan area. Polymerase chain reaction (PCR)-based replicon typing and Southern hybridisations were used to identify the bla(CTX-M-15) location. The genetic environment was analysed by PCR mapping and sequencing, and transferability of bla(CTX-M-15) was evaluated by conjugation and transformation assays. The majority of the 37 isolates carried bla(CTX-M-15) in a plasmid location, frequently associated with the aac(6')-Ib-cr gene. Plasmids encoding bla(CTX-M-15) carried three distinct replicons, i.e. IncFII, IncR and IncFIIk, the latter two not having been described previously in association with bla(CTX-M-15). Several of these plasmids were not self-transferable. Furthermore, in all isolates belonging to sequence type ST-1, bla(CTX-M-15) was found integrated into the K. pneumoniae chromosome. In all the studied isolates, the mobile element ISEcp1 was found upstream of bla(CTX-M-15), whereas IS26 was found inserted within ISEcp1 in several isolates, in previously unreported positions. In conclusion, these findings indicate that among K. pneumoniae strains isolated in the Barcelona metropolitan area, bla(CTX-M-15) is associated with diverse genetic elements, including the IncR and IncFIIk replicons, as reported for the first time here, and the chromosome.

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Section: Introductionmentioning

confidence: 65%

Section: Bacterial Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration

Coelho¹,

González-López²,

Miró³

et al. 2010

International Journal of Antimicrobial Agents

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“…Sequencing of both strands of amplicons was performed. The presence of the aac(6=)-Ib-cr, qnrA, qnrB, qnrS, qepA, and oqxAB genes conferring resistance to fluoroquinolones was also assessed (27).…”

Section: Methodsmentioning

confidence: 99%

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Solà-Ginés

González-López

Cameron-Veas

et al. 2015

Appl Environ Microbiol

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bFlies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence of Escherichia coli strains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the resistant population was performed by different methods: MIC determination, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and phylotyping. The presence of extended-spectrum beta-lactamase (ESBL) genes, their plasmid location, and the mobile genetic elements involved in their mobilization were studied. Additionally, the presence of 35 genes associated with virulence was evaluated. Out of 682 flies captured, 42 yielded ESBL-producing E. coli. Of these isolates, 23 contained bla CTX-M-1 , 18 contained bla CTX-M-14 , and 1 contained bla CTX-M-9 . ESBL genes were associated mainly with the presence of the IncI1 and IncFIB replicons. Additionally, all the strains were multiresistant, and five of them also harbored qnrS. Identical PFGE profiles were found for E. coli isolates obtained from flies at different sampling times, indicating a persistence of the same clones in the farm environment over months. According to their virulence genes, 81% of the isolates were considered avian-pathogenic E. coli (APEC) and 29% were considered extraintestinal pathogenic E. coli (ExPEC). The entrance of flies into broiler houses constitutes a considerable risk for colonization of broilers with multidrug-resistant E. coli. ESBLs in flies reflect the contamination status of the farm environment. Additionally, this study demonstrates the potential contribution of flies to the dissemination of virulence and resistance genes into different ecological niches.

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Les entérobactéries productrices de bêta-lactamases à spectre élargi : où sont les dangers ?

Nicolas-Chanoine

2012

Réanimation

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Detection of three stable genetic clones of CTX-M-15-producing Klebsiella pneumoniae in the Barcelona metropolitan area, Spain

Cited by 27 publications

References 7 publications

Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration

Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration

Houseflies (Musca domestica) as Vectors for Extended-Spectrum β-Lactamase-Producing Escherichia coli on Spanish Broiler Farms

Les entérobactéries productrices de bêta-lactamases à spectre élargi : où sont les dangers ?

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