Detection of the toxin domoic acid from clam extracts using a portable surface plasmon resonance biosensor

Stevens, Richard; Soelberg, Scott D.; Eberhart, Bich Thuy L; Spencer, Sylvester; Wekell, John C.; Chinowsky, Timothy M.; Trainer, Vera L.; Furlong, Clement E.

doi:10.1016/j.hal.2006.08.001

Cited by 90 publications

(53 citation statements)

References 32 publications

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“…The curve midpoint value is low (17.9 ng/ml) and similar to those produced by Le Berre and Kane, (2006), Micheli et al, (2004), Stevens et al, (2007) and Yu et al, 2005) who showed midpoints of approximately 20 ng/ml using either optical or electrochemical biosensors. The curve is more sensitive than those produced by Kreuzer et al, (2002) (midpoint 42 ng/ml) and Lotierzo et al, (2004) (midpoint 58 ng/ml), however it is not as sensitive as that produced by Campbell et al, (2011) (midpoint 2.6ng/ml) using a fully automated prototype multichannel array surface plasmon resonance biosensor.…”

Section: Development Of a Domoic Acid Calibration Curvesupporting

confidence: 80%

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

McGrath¹,

Andersson

Campbell³

et al. 2013

Biosensors and Bioelectronics

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A prototype fluorescent based biosensor has been developed for the antibody based detection of food related contaminants. Its performance was characterised and showed a typical antibody binding signal of 200Ͳ2000 mV, a short term noise of 9.1 mV, and baseline slope of Ͳ0.016 mV/s over 4 hours. Bulk signal detection repeatability (n=23) and reproducibility (n=3) were less than 2.4 %CV. The biosensor detection unit was evaluated using two food related model systems proving its ability to monitor both binding using commercial products and inhibition through the development of an assay. This assay development potential was evaluated by observing the biosensor's performance whilst appraising several labelled antibody and glass slide configurations. The molecular interaction between biotin and an antiͲbiotin antibody was shown to be inhibited by 41% due to the presence of biotin in a sample. A food toxin (domoic acid) calibration curve was produced, with %CVs ranging from 2.7 to 7.8%, and a midpoint of approximately 17 ng/ml with further optimisation possible. The ultimate aim of this study was to demonstrate the working principles of this innovative biosensor as a potential portable tool with the opportunity of interchangeable assays. The biosensor design is applicable for the requirements of routine food contaminant analysis, with respect to performance, functionality and cost.

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Section: Development Of a Domoic Acid Calibration Curvesupporting

confidence: 80%

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

McGrath¹,

Andersson

Campbell³

et al. 2013

Biosensors and Bioelectronics

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“…Antibody-based assays have been developed for a variety of HAB toxins and many of these tests are now commercially available (Laycock et al, 2001;Cembella et al, 2003). One novel immunoassay utilizes surface plasmon resonance (SPR) in a portable system developed for rapid field quantification of toxin levels in both shellfish and seawater (Stevens et al, 2007).…”

Section: Detection Of Marine Toxicmentioning

confidence: 99%

Marine biotoxins and its detection

Suresh¹,

Gauri²,

Desai³

et al. 2014

Afr. J. Environ. Sci. Technol.

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The incidences of intoxication due to the consumption of marine foods have been increasing in recent years. This is due to the presence of biotoxins in foods of marine origin. The biotoxins will be accumulated in the marine foods due to the consumption of toxic biota of marine origin. When this contaminated food is taken by the humans or animals, those toxins will be transferred to them causing intoxication and lethality. Among these intoxications, most of them are caused by the harmful algal blooms (HAB). In order to avoid the harmful effects from marine biotoxins, it is necessary to have the proper knowledge. In this manuscript, the different types of biotoxins, source of intoxication, characteristics of toxins, detection and control measures are discussed in detail.

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“…This portable device was able to reach a detection limit of 3 µg/L (10 nM) and quantifiable range of 4-60 µg/L (13-200 nM) for DA. The method correlates well with the detection of DA in concentrated algal extracts or high dissolved levels in seawater [96].…”

Section: Toxinsmentioning

confidence: 66%

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

2014

J Environ Anal Chem

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The detection and monitoring techniques for algal toxins and the causative harmful algal blooms (HABs) are essential for the protection of aquatic lives, shellfish safety, drinking water quality, and public health. Toward the development of fast, easy, and reliable techniques, much progress has been made during the last decade for the qualitative and quantitative analysis of algal toxins. This review highlights the recent progress and new trends of these analytical and monitoring tools, ranging from in-situ quick screening protocols for the monitoring of algal blooms to mass spectrometric analysis of trace levels of various algal toxins and structural elucidation. Solid-phase adsorption toxin tracking (SPATT) deployed in the field for the passive sampling of algal toxins has been recently validated, and improved ELISA-based methods with lower detection limits for more toxins have become commercially available for both screening and routine monitoring purposes. Liquid chromatography-mass spectrometry with several recent mass spectrometric innovations has expanded our understanding of traditional toxins, their metabolites along with newly discovered toxins of ecological importance. Several established in vivo and in vitro bioassays will continue to be used as benchmark toxicological testing of algal toxins; however, newly emerged molecular probing techniques such as real-time quantitative polymerase chain reaction (qPCR) have extended our ability to trace algal toxins from causative organisms at the molecular level. New chemical and biological sensors, lab-on-chip and remote sensing of blooms being developed will hold promise for early warning and routine monitoring to better manage and protect our freshwater, coastal and marine resources from adverse impact by harmful algal blooms.

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Detection of the toxin domoic acid from clam extracts using a portable surface plasmon resonance biosensor

Cited by 90 publications

References 32 publications

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

Marine biotoxins and its detection

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

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