Detection of the proto-oncogene eIF4E in surgical margins may predict recurrence in head and neck cancer

Nathan, Cherie Ann O.; Liu, Li; Li, Benjamin D.; Abreo, Fleurette; Nandy, Indrani; Benedetti, Arrigo De

doi:10.1038/sj.onc.1201216

Cited by 168 publications

(159 citation statements)

References 11 publications

Supporting

Mentioning

151

Contrasting

Unclassified

Order By: Relevance

“…A relation between translational control and malignant cell transformation became obvious, when elevated levels of eIF-4E as well as reduced levels of the eIF-2a kinase, PKR, were detected in cancer cells (Kerekatte et al, 1995;Nathan et al, 1997;Jagus et al, 1999). Furthermore, overexpression of eIF-4E induced transformation (Rhoads, 1993), and its inhibition by antisense RNA decreased the malignancy of transformed cells (Graff et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1

Eberle

et al. 2002

Br J Cancer

View full text Add to dashboard Cite

Control of translation initiation was recognised as a critical checkpoint for cell proliferation and tumorigenesis. In human melanoma cells, we have previously reported consistent overexpression of translation initiation factor eIF-4A1. Here, we investigated by transfection of antisense constructs its significance for the control of melanoma cell growth. The tetracyclineinducible expression system was established in melanoma cells, and three fragments of the 5'-, central-, and 3'-portion of the eIF-4A1 cDNA were subcloned in antisense and in sense orientation after a tetracycline inducible promoter. Significant proliferation decrease was obtained after transient transfection and induction of antisense RNA directed against the 5'-and the central portion (up to 10%), whereas, no effects were seen after induction of the 3'-fragment and the sense controls. Cell clones stably transfected with the central antisense fragment revealed after doxycycline induction reduced expression of endogeneous eIF-4A1 mRNA correlated with decreased proliferation rates (up to 6%). These data demonstrate the applicability of antisense strategies against translation factors in melanoma cells. Translation initiation factor eIF-4A1 contributes to the control of melanoma cell proliferation and may be taken into consideration when scheduling new therapeutic approaches targeting the translational control.

show abstract

Section: Discussionmentioning

confidence: 99%

Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1

Eberle

et al. 2002

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…1 It is overexpressed in a wide variety of cancers including breast, head and neck squamous cell carcinoma, non-Hodgkin's lymphomas and a subset of myeloid leukemias. 20,[23][24][25][26] Increased levels of eIF4E are an indicator of poor prognosis in many of these including breast cancer. 27,28 eIF4E overexpression leads to oncogenic transformation of cell lines 29 and overexpression of eIF4E leads to increased tumour size and increased metastases in xenograft mouse models.…”

Section: Physiological and Biological Implications Of Rna Regulonsmentioning

confidence: 99%

Controlling Gene Expression through RNA Regulons: The Role of the Eukaryotic Translation Initiation Factor eIF4E

Čuljković¹,

Topisirović²,

Borden³

2007

Cell Cycle

137

157

View full text Add to dashboard Cite

“…4,5 To overcome this problem, the design of an effective cancer gene therapy that permits selective killing of both the primary tumor and distant metastasis must exploit a general characteristic that distinguishes cancer cells from normal cells. Increased expression of the translation initiation factor /proto -oncogene eIF4E has been shown to occur in bladder, 6 breast, 7 cervical, 8 colon, 9 head and neck, 10 and prostate tumors. 11 Therefore, we exploited the ubiquitously increased expression of eIF4E in solid tumors to develop a novel, genetherapy strategy.…”

mentioning

confidence: 99%

A cancer gene therapy approach through translational control of a suicide gene

2002

Self Cite

View full text Add to dashboard Cite

The translation initiation factor eIF4E is elevated in most solid tumors resulting in translation of mRNAs that are normally repressed by their structured 5 0 untranslated region. We have introduced a translational repressor element in a vector ( BK -UTK ) designed to express herpes thymidine kinase ( HTK ). This and a control vector ( BK -TK ) were used to treat experimental tumors of a murine breast cancer line. Both vectors were equally effective in reducing subcutaneous tumors and lung metastases following ganciclovir administration. However, the BK -TK vector was found to be highly toxic, resulting in severe weight loss, degeneration of various organs, and early death of mice following systemic vector delivery, whereas the BK -UTK increased mean survival without toxicity.

show abstract

Detection of the proto-oncogene eIF4E in surgical margins may predict recurrence in head and neck cancer

Cited by 168 publications

References 11 publications

Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1

Decreased proliferation of human melanoma cell lines caused by antisense RNA against translation factor eIF-4A1

Controlling Gene Expression through RNA Regulons: The Role of the Eukaryotic Translation Initiation Factor eIF4E

A cancer gene therapy approach through translational control of a suicide gene

Contact Info

Product

Resources

About