Detection of the novel IMP-38 among carbapenemase-producing Enterobacteriaceae in a university hospital, China

Jian, Zijuan; Liu, Yanming; Liu, Wenen; Li, Hongling; Zhang, Yunli; Li, Yanbing; Gu, Xiumei; Peng, Wanchan

doi:10.3855/jidc.4179

Cited by 10 publications

(10 citation statements)

References 16 publications

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“…1). Similar results have also been reported in other studies from China [9, 13, 14, 16, 20]. These studies showed that the effects of IMP hydrolase on different carbapenems may be diverse, and therefore, the carbapenem-sensitive phenotype does not mean negative for carbapenemase.…”

Section: Discussionsupporting

confidence: 89%

“…Therefore, IMPKpn exhibited sporadic prevalence in our hospital. In China, IMPKpn isolates have been identified across the country, and the main variants are IMP-4 and IMP-8 [3, 6, 7, 9–21], while IMP-26 and IMP-38 have also been identified [16, 18]. In this study, besides the scattered emergence of IMP-1, IMP-26 and IMP-38, we found that the majority of IMPKpn isolates produced IPM-4 (Fig.…”

Section: Discussionmentioning

confidence: 55%

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Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital

Lai

Guo

et al. 2017

Ann Clin Microbiol Antimicrob

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BackgroundIMP-producing Klebsiella pneumoniae (IMPKpn) exhibits sporadic prevalence in China. The mechanisms related to the spread of IMPKpn remain unclear.MethodsCarbapenem non-susceptible K. pneumoniae isolates were collected from our hospital. The genetic relatedness, antimicrobial susceptibility, as well as sequence types (ST) were analyzed by pulsed-field gel electrophoresis (PFGE), VITEK 2 AST test Kit, and multilocus sequence typing (MLST), respectively. S1-PFGE, Southern blot analysis and multiple PCR amplification were used for plasmid profiling.ResultsBetween October 2009 and June 2016, 25 non-repetitive IMPKpn isolates were identified. PFGE results showed that these isolates belonged to 20 genetically unrelated IMPKpn strains. Diverse STs were identified by MLST. Most strains carried bla IMP-4, followed by bla IMP-1. Four incompatibility types of bla IMP-carrying plasmids were identified, which included A/C (n = 2), B/O (n = 2), L/M (n = 1) and N (n = 14), while type of other one plasmid failed to be determined.ConclusionsThe IMPKpn isolates exhibited sporadic prevalence in our hospital. IncN types of plasmids with various sizes have emerged as the main platform mediating the spread of the bla IMP genes in our hospital.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 55%

Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital

Lai

Guo

et al. 2017

Ann Clin Microbiol Antimicrob

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“…reported that strains producing the IMP-type carbapenemase were the main cause for the carbapenem resistance in K. pneumoniae in our hospital. [11] However, in our previous studies, CR-KP isolates collected during the outbreak were all shown to produce KPC and to be resistant to all antimicrobial agents tested except for colistin and cotrimoxazole. Thus, these isolates were found to be extensively drug-resistant strains with limited treatment options for infections due to these strains.…”

Section: Introductionmentioning

confidence: 94%

An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital

Zou

Wang

et al. 2016

Chinese Medical Journal

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Background:Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae bacteria, which cause serious disease outbreaks worldwide, was rarely detected in Xiangya Hospital, prior to an outbreak that occurred from August 4, 2014, to March 17, 2015. The aim of this study was to analyze the epidemiology and molecular characteristics of the K. pneumoniae strains isolated during the outbreak.Methods:Nonduplicate carbapenem-resistant K. pneumoniae isolates were screened for blaKPC-2 and multiple other resistance determinants using polymerase chain reaction. Subsequent studies included pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, analysis of plasmids, and genetic organization of blaKPC-2 locus.Results:Seventeen blaKPC-2-positive K. pneumoniae were identified. A wide range of resistant determinants was detected. Most isolates (88.2%) coharbored blaKPC-2 and rmtB in addition to other resistance genes, including blaSHV-1, blaTEM-1, and aac(3)-IIa. The blaKPC-2 and rmtB genes were located on the conjugative IncFIB-type plasmid. Genetic organization of blaKPC-2 locusin most strains was consistent with that of the plasmid pKP048. Four types (A1, A2, A3, and B) were detected by PFGE, and Type A1, an ST11, was the predominant PFGE type. A novel K. pneumoniae sequence type (ST1883) related to ST11 was discovered.Conclusions:These isolates in our study appeared to be clonal and ST11 K. pneumoniae was the predominant clone attributed to the outbreak. Coharbing of blaKPC-2 and rmtB, which were located on a transferable plasmid, in clinical K. pneumoniae isolates may lead to the emergence of a new pattern of drug resistance.

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“…The yearly frequencies of IMP-38-producing K. pneumoniae clinical isolates were 2.04% (1/49) in 2013, 0.0% (0/82) in 2014, 2.72% (2/88) in 2015, and 3.77% (11/296) in 2016. The results suggested that the IMP-38-producing isolates had persisted in our hospital since the initial identification (12).…”

Section: Resultsmentioning

confidence: 82%

“…IMP carbapenemase, a member of the metallo-␤-lactamases capable of hydrolyzing almost all ␤-lactams, was first described in Japan in the 1990s (11). In 2011, our group first identified a novel IMP-38 variant in the neonatology department in our hospital (12). In this study, we examined the prevalence of bla IMP-38 -harboirng K. pneumoniae isolates in our hospital and determined their genetic backgrounds.…”

mentioning

confidence: 98%

IMP-38-Producing High-Risk Sequence Type 307 Klebsiella pneumoniae Strains from a Neonatal Unit in China

Wang

Zhao

Liu

et al. 2020

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An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among carbapenem-resistant Klebsiella pneumoniae isolates from a tertiary care hospital in central China. A total of 14 IMP-38-producing ST307 K. pneumoniae strains were identified from 2013 to 2016, with 13 strains isolated from patients with neonatal sepsis in the neonatal ward. PacBio and Illumina whole-genome sequencing analysis performed on a representative IMP-38-producing K. pneumoniae strain, WCGKP294, showed that it contained a circular chromosome and two plasmids. Carbapenemase gene blaIMP-38 is colocated with blaCTX-M-3 in transposon Tn6382 on an IncHI5 plasmid (pWCGKP294-2). WCGKP294 harbors another IncFIB plasmid, pWCGKP294-1, carrying three copies of tandem-repeated IS26-blaSHV-2A-deoR-ygbJ-ygbK-fucA-IS26 composite transposon elements. Phylogenetic analysis placed WCGKP294 in the global ST307 cluster, distant from the U.S. (Texas) and South Africa clusters. Nevertheless, WCGKP294 does not contain the chromosomal fluoroquinolone resistance-associated mutations and IncFIIK/IncFIBK plasmid-associated blaCTX-M-15 gene that are frequently found in other global ST307 strains. IMPORTANCE We described the genome and resistome characterization of a carbapenem-resistant Klebsiella pneumoniae ST307 strain carrying blaIMP-38 in China. This report highlights that the high-risk ST307 clone continues to acquire different antimicrobial resistance genes, posing significant challenges to clinical practice, and should be closely monitored.

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Detection of the novel IMP-38 among carbapenemase-producing Enterobacteriaceae in a university hospital, China

Cited by 10 publications

References 16 publications

Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital

Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital

An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital

IMP-38-Producing High-Risk Sequence Type 307 Klebsiella pneumoniae Strains from a Neonatal Unit in China

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