Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods

Mouratidou, Theoni; Kaniou-Grigoriadou, I.; Samara, Constantini

doi:10.1016/j.scitotenv.2005.03.002

Cited by 48 publications

(18 citation statements)

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“…DSP due to toxic mussel consumption affected by the presence of high numbers of D. acuminata were reported in January 2000 (Economou et al 2007). Okadaic acid from mussels in Thermaikos Gulf has been quantified and found that okadaic acids concentrations reached maximum values of 36 μg g −1 in the hepatopancreas of mussels (Mouratidou et al 2006). Concentrations of A. minutumin in Thermaikos Gulf during 2002 were 1.0×10 3 cells l −1 (average) and 4.2×10 3 cells l −1 (maximum) (Ignatiades et al 2007).…”

Section: Nutrient River Discharges To the Mediterraneanmentioning

confidence: 99%

Eutrophication and environmental policy in the Mediterranean Sea: a review

Karydis

Kitsiou

2011

Environ Monit Assess

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The Mediterranean Sea is a semienclosed basin connected with the open sea mainly through the Strait of Gibraltar. Due to the circulation pattern and the long residence time ranging between 80 and 100 years, the Mediterranean Sea is a sensitive environment to eutrophication pressures. The main body of water of the Mediterranean is characterized by very low nutrient concentrations, and therefore, the Mediterranean is classified among the most oligotrophic (very poor waters in nutrients) seas of the world's oceans. However, some coastal areas, mainly in the northern part of the basin, receive excessive loads of nutrients from sewage effluents, river fluxes, aquaculture farms, fertilizers, and industrial facilities, showing intense eutrophic phenomena with many adverse effects for the marine ecosystem and humans. Various national and international authorities, in addition to monitoring, have taken legal and administrative measures to mitigate eutrophication trends in the area. The Mediterranean environment is a good paradigm of integration of extensive legal framework, scientific knowledge, and administrative practices. The Barcelona Convention, the Mediterranean Action Plan, and European Union Directives on water quality and coastal management, together with scientific information derived from international research programs in the Mediterranean, provide a sound background for practical actions in eutrophication problems. In the present work, the problem of coastal eutrophication in the Mediterranean is reviewed in connection with public policies of the Mediterranean States based on national and international legislation and scientific knowledge on Mediterranean oceanography-ecology and actions coordinated by international bodies. These common actions and practices on coastal management are also discussed in relation to the need for sustainable development and protection of the coastal zone in the Mediterranean Sea.

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Section: Nutrient River Discharges To the Mediterraneanmentioning

confidence: 99%

Eutrophication and environmental policy in the Mediterranean Sea: a review

Karydis

Kitsiou

2011

Environ Monit Assess

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“…The occurrence of Dinophysis spp., especially Dinophysis acuta, D. acuminata and Dinophysis fortii, have been linked to the accumulation of OA group toxins and DSP in Asia (Yasumoto et al, 1978;Mak et al, 2005), North America (Subba Rao et al, 1993;Campbell et al, 2010), South America (Zhao et al,1993;Garcia et al, 2004), Australasia (MacKenzie et al, 1998Madigan et al, 2006), North of Europe (Torgersen et al, 2005;Turrell and Stobo, 2007;Fux et al, 2009) and South of Europe (Boni et al, 1993;Reguera et al, 1993;Gago-Martinez et al, 1993;Mouratidou et al, 2006). DSP symptoms include nausea, vomiting, diarrhea, and abdominal pain.…”

Section: Introductionmentioning

confidence: 99%

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Fux

Smith

Tong

et al. 2011

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a b s t r a c tMarine dinoflagellates of the genus Dinophysis can produce toxins of the okadaic acid (OA) and pectenotoxin (PTX) groups. These lipophilic toxins accumulate in filter-feeding shellfish and cause an illness in consumers called diarrhetic shellfish poisoning (DSP). In 2008, a bloom of Dinophysis led to the closure of shellfish harvesting areas along the Texas coast, one of the first DSP-related closures in the U.S. This event resulted in a broad study of toxin production in isolates of Dinophysis spp. from U.S. waters. In the present study, we compared toxin profiles in geographical isolates of Dinophysis collected in the U.S. (Eel Pond, Woods Hole MA; Martha's Vineyard, MA; and Port Aransas Bay, Texas), and in those from Canada (Blacks Harbour, Bay of Fundy) and Chile (Reloncavi Estuary), when cultured in the laboratory under the same conditions. For each isolate, the mitochondrial cox1 gene was sequenced to assist in species identification. Strains from the northeastern U.S. and Canada were all assigned to Dinophysis acuminata, while those from Chile and Texas were most likely within the D. acuminata complex whereas precise species designation could not be made with this marker. Toxins were detected in all Dinophysis isolates and each isolate had a different profile. Toxin profiles of isolates from Eel Pond, Martha's Vineyard, and Bay of Fundy were most similar, in that they all contained OA, DTX1, and PTX2. The Eel Pond isolate also contained OA-D8 and DTX1-D7, and low levels (unconfirmed structurally) of DTX1-D8 and DTX1-D9. D. acuminata from Martha's Vineyard produced DTX1-D7, along with OA, DTX1, and PTX2, as identified in both the cells and the culture medium. D. acuminata from the Bay of Fundy produced DTX1 and PTX2, as found in both cells and culture medium, while only trace amounts of OA were detected in the medium. The Dinophysis strain from Texas only produced OA, and the one from Chile only PTX2, as confirmed in both cells and culture medium.Published by Elsevier Ltd.

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“…Its biological traits, such as fast growth, immunity to parasites and disease, stress tolerance, and high survivorship (Branch and Steffani, 2004), in combination with its preferred environmental conditions prevailing in Greece, have greatly contributed to the large development of Greek mussel populations over the past decades (Smaal, 2002), which have become a very important resource for the national economy. The annual mussel production in Greece is approximately 40,000 tons, of which more than 80% comes from the Thermaikos Gulf (Mouratidou et al, 2006). The Thermaikos Gulf is a semi-closed bay in the Northwestern Aegean Sea, which is plentiful in organic material due to a delta formed by the deposition of the 4 rivers within it.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and evaluation of anthropogenic impacts on genetic patterns in cultured and wild populations of mussels (Mytilus galloprovincialis) from Greece

Giantsis¹,

Kravva²,

Apostolidis³

2012

Genet. Mol. Res.

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ABSTRACT. Despite the great commercial and economic importance of mussels, Mytilus galloprovincialis (Bivalvia, Mollusca) in Greece, little information is available concerning their population genetic structure. We used RAPD markers to examine genetic differentiation and potential impact of aquaculture practices and other anthropogenic activities on the genetic structure of two cultivated and eight wild mussel populations collected from one Turkish and nine Greek coast sites. Five random decamer primers were chosen, among 34 tested, for the analysis of 433 individuals. Eighty-eight bands (genetic loci) were scored, all of which were polymorphic. No indication of reduced genetic variability was observed in the cultured populations. In contrast, a loss in genetic diversity was observed in populations from two localities (Canakkale and Kalochori) that are heavily polluted by chemical contaminants. analyses and exact tests revealed significant heterogeneity among M. galloprovincialis population samples, although their genetic divergence seemed to be independent of geographic distances. Anthropogenic activities, i.e., marine pollution and transplantation of mussels, appear to have played an important role in shaping patterns of genetic diversity and differentiation among Greek M. galloprovincialis populations.

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Detection of the marine toxin okadaic acid in mussels during a diarrhetic shellfish poisoning (DSP) episode in Thermaikos Gulf, Greece, using biological, chemical and immunological methods

Cited by 48 publications

References 18 publications

Eutrophication and environmental policy in the Mediterranean Sea: a review

Eutrophication and environmental policy in the Mediterranean Sea: a review

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Genetic characterization and evaluation of anthropogenic impacts on genetic patterns in cultured and wild populations of mussels (Mytilus galloprovincialis) from Greece

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