Detection of the linalool‐producing <i>NES1</i> variant across diverse strawberry (<i>Fragaria</i> spp.) accessions

Chambers, Alan H.; Whitaker, Vance M.; Gibbs, Brian; Plotto, Anne; Folta, Kevin M.

doi:10.1111/j.1439-0523.2012.01959.x

Cited by 18 publications

(14 citation statements)

References 20 publications

(46 reference statements)

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“…Before analysis, samples were held at 10 °C in a Peltier cooling tray attached to a MPS2 autosampler (Gerstel). All other volatile sampling and analysis methods were as previously described [ 23 ]. An authentic MA standard (Sigma Aldrich, St. Louis, MO, USA) was run under the same chromatographic conditions as fruit samples for verification of volatile identity.…”

Section: Methodsmentioning

confidence: 99%

Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry

et al. 2017

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BackgroundMethyl anthranilate (MA) contributes an attractive fruity note to the complex flavor and aroma of strawberry (Fragaria spp.), yet it is rare in modern cultivars. The genetic basis for its biosynthesis has not been elucidated. Understanding the specific genes required for its synthesis could allow the development of gene/allele-specific molecular markers to speed breeding of flavorful strawberries.ResultsRipe fruits from individuals in an F1 population resulting from a cross between a MA producer and a non-producer were examined using a bulk-segregant transcriptome approach. MA producer and non-producer transcriptomes were compared, revealing five candidate transcripts that strictly co-segregated with MA production. One candidate encodes an annotated methyltransferase. MA levels are lower when this transcript is suppressed with RNAi, and bacterial cultures expressing the protein produced MA in the presence of anthranilic acid. Frozen fruit powders reconstituted with anthranilic acid and a methyl donor produced MA only if the transcript was detected in the fruit powder. A DNA-based molecular marker was developed that segregates with the MA-producing gene variant.ConclusionsThese analyses indicate that the methyltransferase, now noted ANTHRANILIC ACID METHYL TRANSFERASE (FanAAMT), mediates the ultimate step of MA production in cultivated strawberry. Identification of this gene and its associated molecular marker may hasten breeding efforts to introduce this important volatile into modern cultivars.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1088-1) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry

et al. 2017

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“…The terpenoid linalool appears as an exception with its pleasant freshflowery, citrus-like odor. Linalool was found in higher amounts in cultivars as also described by Chambers et al [5]. These authors postulated a mutation in the nerolidol synthase (FaNES1) occuring in octoploids (F. × ananassa) in contrast to the diploids (F. vesca).…”

Section: Diversity Of Volatile Patterns In One Harvest Seasonmentioning

confidence: 62%

Diversity of metabolite patterns and sensory characters in wild and cultivated strawberries1

Ulrich

Olbricht

2014

JBR

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In breeding programs wild strawberry species are used increasingly to enhance the genetic diversity and to implement higher sensory quality. Next to Fragaria moschata L. and F. viridis Weston wild collections cultivars of Fragaria vesca L. were the standards for strawberry taste in Europe before the development of Fragaria × ananassa Duch. around 250 years ago. Therefore, the objective of this research was the evaluation of patterns of volatile organic compounds including around twenty strawberry character impact compounds evaluated for Fragaria vesca and in comparison for standard cultivars of Fragaria × ananassa. The metabolic patterns were measured using immersion-SBSE-GC-MS. Sixteen accessions of Fragaria vesca exhibit a high diversity in qualities and quantities of volatile organic compounds (VOCs). A specific fruit sampling strategy equalizes environmental caused variation and avoids misleading interpretations. The shown results are valuable for further breeding activities regarding flavor, transcriptomic analyses, studies of biochemical pathways and signaling compounds as well as marker development.

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“…It has an antimicrobial effect and it is present in the total pull of volatile organic compounds (VOCs) of many fruits and flowers. It is confirmed that linalool is the most important compound for strawberry flavor [37,38]. Furanone methyl ether typical of strawberries.…”

Section: 585mentioning

confidence: 72%

Nanomaterial Gas Sensors for Online Monitoring System of Fruit Jams

Núñez-Carmona

Abbatangelo

Zottele

et al. 2019

Foods

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Jams are appreciated worldwide and have become a growing market, due to the greater attention paid by consumers for healthy food. The selected products for this study represent a segment of the European market that addresses natural products without added sucrose or with a low content of natural sugars. This study aims to identify volatile organic compounds (VOCs) that characterize three flavors of fruit and five recipes using gas chromatography–mass spectrometry (GC–MS) and solid-phase micro-extraction (SPME) analysis. Furthermore, an innovative device, a small sensor system (S3), based on gas sensors with nanomaterials has been used; it may be particularly advantageous in the production line. Results obtained with linear discriminant analysis (LDA) show that S3 can distinguish among the different recipes thanks to the differences in the VOCs that are present in the specimens, as evidenced by the GC–MS analysis. Finally, this study highlights how the thermal processes for obtaining the jam do not alter the natural properties of the fruit.

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Detection of the linalool‐producing NES1 variant across diverse strawberry (Fragaria spp.) accessions

Cited by 18 publications

References 20 publications

Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry

Identification of a methyltransferase catalyzing the final step of methyl anthranilate synthesis in cultivated strawberry

Diversity of metabolite patterns and sensory characters in wild and cultivated strawberries1

Nanomaterial Gas Sensors for Online Monitoring System of Fruit Jams

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