Detection of the latent functional insufficiency of dopaminergic neurons in the nigrostriatal system in a chronic model of Parkinson’s disease

Safandeev, V. V.; Колачева, А. А.; Ivanov, D E; Ugryumov, M. V.

doi:10.1134/s1819712417040079

Cited by 5 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also would like to stress an important aspect of the use of saporin-based toxins compared with other lesioning agents (or with the transgenic mice approach)-the use of saporin-based conjugates offers the advantage of producing graded lesions, thus making possible to address compensatory responses by surviving neuronal populations following partial lesions as well as the effects of cell or neurotransmitter replacement strategies following complete ablations [42]. It is also worth to note that animal models with graded lesions may be used to create tests for diagnostics of pathologies at early stages [43].…”

Section: Igg-saporin Conjugatesmentioning

confidence: 99%

Saporin from Saponaria officinalis as a Tool for Experimental Research, Modeling, and Therapy in Neuroscience

Bolshakov

Stepanichev

Dobryakova

et al. 2020

Toxins

View full text Add to dashboard Cite

Saporin, which is extracted from Saponaria officinalis, is a protein toxin that inactivates ribosomes. Saporin itself is non-selective toxin but acquires high specificity after conjugation with different ligands such as signaling peptides or antibodies to some surface proteins expressed in a chosen cell subpopulation. The saporin-based conjugated toxins were widely adopted in neuroscience as a convenient tool to induce highly selective degeneration of desired cell subpopulation. Induction of selective cell death is one of approaches used to model neurodegenerative diseases, study functions of certain cell subpopulations in the brain, and therapy. Here, we review studies where saporin-based conjugates were used to analyze cell mechanisms of sleep, general anesthesia, epilepsy, pain, and development of Parkinson’s and Alzheimer’s diseases. Limitations and future perspectives of use of saporin-based toxins in neuroscience are discussed.

show abstract

Section: Igg-saporin Conjugatesmentioning

confidence: 99%