Detection of the ‘Hyperdynamic State’ of Sepsis in the Baboon during Lethal E. coli Infusion

Hinshaw, Lerner B.; Brackett, Daniel J.; Archer, L. T.; Beller, B. K.; Wilson, M F

doi:10.1097/00005373-198305000-00001

Cited by 32 publications

(19 citation statements)

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“…The arterial blood collected included 0.5 mL of nonanticoagulated blood for glucose measurement [33] and 0.5 mL of whole blood for bacterial colony counts [34]. Not more than 10% of the estimated total blood volume (70 mL/kg) was withdrawn over the first 24 h.…”

Section: Methodsmentioning

confidence: 99%

Staging of the Baboon Response to Group A Streptococci Administered Intramuscularly: A Descriptive Study of the Clinical Symptoms and Clinical Chemical Response Patterns

Taylor

Bryant²,

Blick³

et al. 1999

CLIN INFECT DIS

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Group A streptococcal infections, ranging from necrotizing fasciitis and myositis to toxic shock syndrome, have increased over the last 10 years. We developed the first primate model of necrotizing fasciitis and myositis. Thirteen baboons were inoculated intramuscularly with group A streptococci (GAS). Eleven animals survived for > or = 11 days before sacrifice, and two animals died within 2 days. The site of inoculation of the survivors exhibited an intense neutrophilic influx (stage I), followed by a lymphoplasmacytic influx (stages II and III). This was accompanied by the appearance of markers of an acute and then a chronic systemic inflammatory response. In contrast, the site of inoculation of the two nonsurvivors exhibited intravascular aggregates of neutrophils at its margin with no influx of neutrophils and with extensive bacterial colonization. We conclude that GAS inoculation induces a local and systemic acute neutrophilia followed by a chronic lymphoplasmacytic response; failure, initially, of neutrophilic influx into the site of inoculation predisposes to systemic GAS sepsis and death; and this three-stage primate model approximates the human disease.

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Section: Methodsmentioning

confidence: 99%

Staging of the Baboon Response to Group A Streptococci Administered Intramuscularly: A Descriptive Study of the Clinical Symptoms and Clinical Chemical Response Patterns

Taylor

Bryant²,

Blick³

et al. 1999

CLIN INFECT DIS

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show abstract

“…The blood sampled at each drawing included: 1 nml anticoagulated with ethylenediaminotetraacetic acid for complete blood count (CBC),' he-matocrit, platelet and differential counts; 2.0 ml anticoagulated with 3.8% sodium citrate for fibrinogen (13), whole blood clot lysis (14), rocket immunoelectrophoretic assays of baboon protein C, and HPC-4 (rabbit anti-mouse IgG monoclonal antibody to human protein C, see description below); 1.0 ml in trasylol/thrombin for fibrin degradation products (15); 1.0 ml ofclotted blood for serum glutamic pyruvic transaminase (SGPT) (16); and 1.0 ml at T-0, T+120, and T+360 min for colony counts (17).…”

Section: Methodsmentioning

confidence: 99%

“…E. coli (organisms) Type B were isolated from a stool specimen at Children's Memorial Hospital, Oklahoma City. They were stored in the lyophilized state at 40C after growth in tryptic soybean agar and reconstituted and characterized as described by Hinshaw et al (17).…”

Section: Methodsmentioning

confidence: 99%

Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon.

Taylor¹,

Chang²,

Esmon³

et al. 1987

J. Clin. Invest.

876

536

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“…In many studies baboons (Papio cyanocephalus cynocephalus) have been used to investigate the efficacy of potential therapeutic substances by assessing their ability to improve the survival of a lethal Escherichia coli infusion [2,3]. In such animal models, the effect of the therapeutic intervention on the coagulopathy induced by septicaemia can be defined using welldefined end points, such as the amount of fibrinogen consumption and fibrin deposition [7,8]. However, these models are not sufficiently specific to analyse incisively the involvement of individual coagulation and fibrinolytic factors or inhibitors in the pathogenesis of DIC.…”

Section: Models Of Experimental Bacteraemia or Endotoxaemia And Activmentioning

confidence: 99%

The cytokine‐mediated imbalance between coagulant and anticoagulant mechanisms in sepsis and endotoxaemia

Levi

Poll

Cate

et al. 1997

Eur J Clin Investigation

285

174

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Abstract. Disseminated intravascular coagulation (DIC)is a frequently occurring complication of sepsis and may contribute to multiple organ failure. More insight into the pathogenesis of this derangement of the coagulation system is necessary to develop more effective therapeutic strategies for this condition. Recently, more detailed knowledge on the pathogenetic pathways involved in DIC has been obtained by the study of models of experimental bacteraemia and endotoxaemia in human subjects and non-human primates. The mechanisms that lead to activation of coagulation, potentiated by the simultaneous depression of physiological inhibitory systems and to impaired function of the fibrinolytic system, are outlined in this review. In addition, the mediatory role of various cytokines in the derangement of coagulation is discussed.

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Detection of the ‘Hyperdynamic State’ of Sepsis in the Baboon during Lethal E. coli Infusion

Cited by 32 publications

References 0 publications

Staging of the Baboon Response to Group A Streptococci Administered Intramuscularly: A Descriptive Study of the Clinical Symptoms and Clinical Chemical Response Patterns

Staging of the Baboon Response to Group A Streptococci Administered Intramuscularly: A Descriptive Study of the Clinical Symptoms and Clinical Chemical Response Patterns

Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon.

The cytokine‐mediated imbalance between coagulant and anticoagulant mechanisms in sepsis and endotoxaemia

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