Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected mice

Amemiya, Kei; Meyers, Jennifer; DeShazer, David; Riggins, Renaldo; Halasohoris, Stephanie; England, Marilyn J.; Ribot, Wilson J.; Norris, Sarah L.; Waag, David M.

doi:10.1016/j.diagmicrobio.2007.04.017

Cited by 23 publications

(24 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…9 The present study showed that antibodies against GroEL (BPSL2697/BMA2001) were increased 4.8-fold while antibodies against DnaK (BPSL2827/BMA2326) were increased~1.4-fold ( Table 1; Table S1). The difference in observed levels of antibodies against GroEL may reflect sensitivity differences between the technologies.…”

mentioning

confidence: 52%

“…8 This case has been the subject of previous reports due in part to the unique opportunity to study a human glanders infection for which pre-exposure and postexposure serum exists. 9,10 A recent analysis indicated that levels of B. mallei-specific IgA, IgG, and IgM were highly elevated at 64 d post-infection, 10 but the immunogenic antigens were not identified. We employed a previously described B. pseudomallei protein array 6,11 to perform an in-depth analysis of this serum.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Distinct human antibody response to the biological warfare agentBurkholderia mallei

et al. 2012

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 52%

mentioning

confidence: 99%

Distinct human antibody response to the biological warfare agentBurkholderia mallei

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Genes encoding 6 of the 31 diagnostic signature proteins (BPSS0476; GroES: BPSL1445; putative lipoprotein BPSL2017; Di-heme cytochrome c peroxidase; BPSL2697; GroEL: BPSL3319; flagellin; and BPSL2520: putative exported protein) were upregulated in vivo. Chaperones and stress response proteins from a range of pathogens have frequently been found to react with the appropriate convalescent sera (14,(24)(25)(26)(27)(28). Both GroEL and DNA K have previously been identified as reactive with sera from melioidosis patients (14,25).…”

Section: E-02mentioning

confidence: 99%

A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens

Felgner

Kayala

Vigil

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

161

225

View full text Add to dashboard Cite

Understanding the way in which the immune system responds to infection is central to the development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, and identified 170 reactive antigens. This subset of antigens was printed on a smaller array and probed with a collection of 747 individual sera derived from 10 patient groups including melioidosis patients from Northeast Thailand and Singapore, patients with different infections, healthy individuals from the USA, and from endemic and nonendemic regions of Thailand. We identified 49 antigens that are significantly more reactive in melioidosis patients than healthy people and patients with other types of bacterial infections. We also identified 59 cross-reactive antigens that are equally reactive among all groups, including healthy controls from the USA. Using these results we were able to devise a test that can classify melioidosis positive and negative individuals with sensitivity and specificity of 95% and 83%, respectively, a significant improvement over currently available diagnostic assays. Half of the reactive antigens contained a predicted signal peptide sequence and were classified as outer membrane, surface structures or secreted molecules, and an additional 20% were associated with pathogenicity, adaptation or chaperones. These results show that microarrays allow a more comprehensive analysis of the immune response on an antigen-specific, patient-specific, and population-specific basis, can identify serodiagnostic antigens, and contribute to a more detailed understanding of immunogenicity to this pathogen.antigen discovery ͉ melioidosis ͉ diagnostic ͉ antigen prediction

show abstract

“…De acordo com Amemiya et al (2007), Al-Ani & Roberson (2007), a PPD-maleína deve ter concentração de proteínas ajustadas para uso na maleinização não inferior a 0,95mg/ ml e não mais que 1,05mg/ml. Após a produção das PPDs--maleína estas foram ajustadas para 1,0mg/ml, de acordo com recomendação da OIE (2008).…”

Section: Resultsunclassified

Produção e purificação parcial de PPD-maleína para diagnóstico do mormo em equídeos

Silva

Campos-Takaki

Teles³

et al. 2014

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Pesq. Vet. Bras. 34(1):57-61, janeiro 2014 57 RESUMO.-Objetivou-se com este estudo produzir e purificar parcialmente a PPD-maleína a partir de amostras de Burkholderia mallei isoladas de equídeos no Brasil com potencial para uso no diagnóstico do mormo. As linhagens de B. mallei fenotipicamente caracterizadas e de virulência comprovada foram inoculadas em caldo Dorset-Henley para crescer e metabolizar. Em seguida, as proteínas foram separadas por precipitação com ácido tricloroacético e precipitadas com sulfato de amônia. As PPDs-maleínas foram concentradas em 1,0mg/ mL e na avaliação realizada em cobaios foi eficaz no desenvolvimento da hipersensibilidade do tipo tardia e consequentemente na identificação de animais verdadeiro positivos e exclusão dos verdadeiro negativos, sendo uma possibilidade em potencial para utilização no diagnóstico do mormo. INTRODUÇÃOO mormo é considerado a principal doença bacteriana dos equídeos, sendo transmissível ao homem e a outras espé-cies animais. Registrada sua reemergência no Brasil (Mota et al. 2000), atualmente a doença constitui um sério problema sanitário para os equídeos das regiões Norte e Nordeste do País (Mota et al. 2005, Brasil 2008 The objective of this study was to produce and partially purify Malleo-protein from Burkholderia mallei samples isolates from Equidae in Brazil with potential for use in the diagnosis of glanders. The strain B. mallei phenotypically characterized and proven virulent was inoculated into broth Dorset-Henley to grow and metabolize. The proteins were separated by trichloroacetic acid precipitation and amonium sulfate precipitation. The PPD mallein was concentrated 1.0mg/mL and biologically tested in guinea pigs. It was effective in the development of delayed-type hypersensitivity and consequently to identify true--positive animals and to exclude of true negatives. There is the possibility for potential use in the glanders diagnosis in Equidae.

show abstract

Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected mice

Cited by 23 publications

References 37 publications

Distinct human antibody response to the biological warfare agentBurkholderia mallei

Distinct human antibody response to the biological warfare agentBurkholderia mallei

A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens

Produção e purificação parcial de PPD-maleína para diagnóstico do mormo em equídeos

Contact Info

Product

Resources

About