Detection of the hbl complex genes in Bacillus cereus isolated from cow raw milk in northwest of Iran

Banaei, R. Alipour; Mahdavi, Saman

doi:10.33899/ijvs.2020.126120.1238

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Following the manufacturer's instructions, the process was carried out. DNA quality was measured spectrophotometrically, and low concentration samples (lower than 100 ng/μL) were eliminated from further analyses (23).…”

Section: Extraction Of Dnamentioning

confidence: 99%

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Arif

2022

IJVS

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Abortion is one of the most critical factors affecting lambing rates and, as a result, sheep farm profitability. It is also significant from a zoonotic viewpoint, in addition to financial losses. In sheep flocks, Campylobacter fetus causes infectious infertility, embryonic death, and miscarriages. The study investigated C. fetus from aborted fetuses and vaginal swab samples collected from sheep flocks in the Sulaimani province by the polymerase chain reaction. Thirty-eight aborted fetuses and 70 vaginal swabs were collected from sheep flocks in three districts of Sulaimani province (Kalar, Said Sadiq, and Chamchamal) from March 2018 to June 2019. The pathogen was identified in clinical specimens using conventional PCR. C. fetus was isolated in 16 of 38 aborted fetuses 42.1% and 13 of 70 vaginal swabs from aborted ewes 18.6%. The C. fetus gene 16S rRNA was sequenced and received the accession number MW694741 in NCBI GenBank. Phylogenetic analysis of 16S rRNA gene sequences designated that the C. fetus isolates formed a separate branch displayed the highest similarity and clustered with MN203686.1 and EU773268.1 accessions in a specific clade. A lower degree of affinity of C. fetus was revealed with Campylobacter coli and Campylobacter jejuni.

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Section: Extraction Of Dnamentioning

confidence: 99%

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Arif

2022

IJVS

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“…During the processing of dairy product, such procedures are required to reduce bacterial contamination (22). We found that only 12/50 (24%) were diagnosed as S. aureus.…”

Section: Discussionmentioning

confidence: 83%

Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

Taher¹,

Yassin²,

Abdulkareem³

2021

IJVS

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Staphylococcus aureus is a microbe associated with human's food poisoning. It is caused by isolates producing different heat stable enterotoxins which act as one of the most spread worldwide gastroenteritis causes. The goal of the current study was to assess the incidence of S. aureus (enterotoxigenic) in the raw milk samples of cows which were collected from various places of Baghdad city. Isolation and identification of S. aureus were done by conventional laboratory method and the diagnoses were confirmed by using molecular method RT-PCR to detect the specific gene of S. aureus nuc gene. In addition, we investigate the occurrence of some of Staphylococcal enterotoxins genes such as SEA, SEB, SEC, SED, and SEE. We found that 12/50 (24%) of the isolates were S. aureus and these isolates carried one or more of the enterotoxin genes such as enterotoxin type SEC 12/12 (100) followed by enterotoxin type SEA 7/12 (58.33%) and only 1/12 (8.33%) was type SED, while type SEB and SEE were negative 0%. Some of the isolates have genes that coded for two enterotoxins patterns such as SEA and SEC 7/12 (58.33%) and SEC and SED 1/12 (8.33%). Fewer of the isolates expressed only one genotype of enterotoxin gene like SEC 4/12 (33.3%). This study has proven that there was a high chance of occurrence for the enterotoxigenic S. aureus at the raw milk of cows in Baghdad city.

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Detection of the hbl complex genes in Bacillus cereus isolated from cow raw milk in northwest of Iran

Cited by 2 publications

References 14 publications

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

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