Detection of the G3 genotype of Echinococcus granulosus from hydatid cysts of Chilean cattle using COX1and ND1mitochondrial markers

Abstract

For a deeper understanding of the phylogenetic relationships of Echinococcus genotypes and species in different intermediate hosts, we analyzed samples from human and bovine hydatid cysts. For this, segments of the cytochrome oxidase (COX1) and NADH dehydrogenase (ND1) mitochondrial genes were used. To obtain sufficient amounts of the ND1 marker to be sequenced properly, a new variant of the PCR assay was implemented. Phylogenetic analysis with both markers showed that most of the analyzed samples correspond to genotype G1. However, a sample from cysts of a bovine lung (Q21), with the COX1 marker, was grouped in a node together with a sample belonging to genotype G3. In the phylogenetic tree obtained with the ND1 marker, this sample was grouped with sequences of genotypes G3, G2, and G4. Analyzing the single nucleotide polymorphic (SNP) sites of both markers, it was observed that the Q21 sequence is almost identical to the G3 sequence and differ in only one SNP from the G2 sequence, and is completely different from G4. These results are noteworthy, since neither G2 nor G3 genotypes have been described previously in Chile, raising the possibility that the G3 genotype is present in these latitudes. This information is highly relevant; it can be employed to uncover additional unknown details of transmission cycles of this important parasite.

Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence

“…The G3 genotype of E. granulosus has been previously detected from buffalo, cattle, sheep, and human isolates from Italy, Turkey, India, and Chile (Casulli et al 2008;Espinoza et al 2014;Sharma et al 2013a, b;Simsek et al 2011). In addition to the G1 genotype, we also detected the G3 variant in a small number of the isolates (4.65 %).…”

High intraspecific variability of Echinococcus granulosus sensu stricto in Chile

“…Manterola et al [5], studied 20 human samples, confirming the presence of E. granulosus s.s. in 19 isolates and E. canadensis (G6 genotype) in a single case following the sequencing of a 366bp section of the cox1 gene and a 471bp section of the nad1 gene. Subsequently, using a similar methodology, Espinoza et al [6] analysed 15 hydatid cysts from cattle and humans, finding that they were all E. granulosus s.s.…”

Aspectos Generales del Perfil Proteómico del Echinococcus granulosus

“…Términos ontológicos del proceso biológico, función molecular y subcategorías de componentes celulares de genes asociados con proteínas del parásitos identificadas en el fluido de un quiste hidatídico mediante Blast2Go. G3, G5 y G6) (Manterola et al, 2008;Espinoza et al, 2014;Alvarez Rojas et al, 2017;Corrêa et al, 2018;Hernández et al, 2019); puesto que la existente (Providean Hidatil EG 95Ò), fue desarrollada por utilizando una pequeña fracción proteica de la membrana exterior del parásito; por lo cual presenta una serie de limitaciones, por ejemplo no otorgar similar cobertura en otros genotipos como G6 y G7, pues fue desarrollada para G1; y no da cobertura a la gran variabilidad de haplotipos descritos (Chow et al, 2008;Alvarez Rojas et al, 2013).…”