Detection of the changes in protein distribution in rat serum after partial hepatectomy using two-dimensional electrophoresis under non-denaturing conditions

Kadofuku, Tsuyoki; Iijima, Takeru; Sato, Tsuneo; Tei, Ichiyo; Makino, Yoshiaki

doi:10.1016/s0378-4347(00)84345-5

Cited by 9 publications

(1 citation statement)

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“…Special attention was then devoted to high‐ and low‐density lipoproteins (HDL and LDL), the lipoprotein classes compatible with migration in low concentration PAA 5, 6; information on the apolipoprotein components was then obtained on the same samples through 2‐DE with native 1d, denaturing 2d (Section 2.2) as well as through 2‐DE with denaturing 1d, denaturing 2d. Further basic studies involved rat serum under different experimental conditions (treatment with carbon tetrachloride 7, hepatectomy 8). α‐Amylase isoforms could be typed in human body fluid samples (serum, saliva, pancreatic juice and urine) 9, taking advantage of the unaltered activity of the enzyme.…”

Section: Analysis Of Protein Samples Under Native Conditionsmentioning

confidence: 99%

Other than IPG‐DALT: 2‐DE variants

2010

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Special problems require special solutions. That is why, despite the trend towards standardization of the procedures in proteomic investigations, a number of alternative protocols, based on 2-DE, have been devised with time and are applied to the resolution of proteins on which the performance of IPG-DALT is poor. The difficult-to-handle samples include high pI, high molecular size and high hydrophobicity proteins. The protocol variants entail changes in the gel media and/or the use of unusual additives (often surfactants or solvents). But also: Specific questions require specific answers. When the aim is not the resolution of individual polypeptide chains in a fully unfolded state but the analysis of higher-order structures (proteins assembled from subunits, complexes assembled from interacting proteins) the standard conditions under which IPG-DALT is performed turn inadequate. Electrophoresis is then performed either in first dimension or in both first dimension and second dimension under non-denaturing (native) and/or non-reducing conditions.

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